Genome-Wide Identification of CsATGs in Tea Plant and the Involvement of CsATG8e in Nitrogen Utilization

Huang, Wei; Ma, Danni; Liu, Hongling; Luo, Jie; Wang, Pu; Wang, Mingle; Guo, Fei; Wang, Yu; Zhao, Hua; Ni, Dejiang

doi:10.3390/ijms21197043

Cited by 11 publications

(9 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in-depth study of autophagy in TB contributes to understanding the molecular mechanism of plant response to abiotic stress and promotes the breeding of excellent stress-resistant varieties. Besides Arabidopsis , autophagy genes are also found in higher plants, such as 32 OsATGs in rice [ 19 ], 26 CsATGs in Sweet Orange [ 20 ], 80 CsATGs in tea [ 21 ], etc. We screen 49 FtATGs genes in the TB genome.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of ATG Gene Family Members in Fagopyrum tataricum and Their Expression during Stress Responses

Fang

Wang

et al. 2022

IJMS

View full text Add to dashboard Cite

Abiotic stresses such as drought and salinity are major environmental factors limiting plant productivity. Autophagy-related genes are extensively involved in plant growth, development, and adverse stress responses, which have not yet been characterized in Tartary buckwheat (Fagopyrum tataricum, TB). In this study, we verified that drought stress could induce autophagy in TB roots. Next, 49 FtATGs in the whole genome of TB were identified. All FtATGs were randomly distributed in 8 known chromosomes, while 11 FtATGs were predictably segmental repeats. As the core component of autophagy, there were 8 FtATG8s with similar gene structures in TB, while FtATG8s showed high expression at the transcription level under drought and salt stresses. The cis-acting element analysis identified that all FtATG8 promoters contain light-responsive and MYB-binding elements. FtATG8s showed a cell-wide protein interaction network and strongly correlated with distinct stress-associated transcription factors. Furthermore, overexpression of FtATG8a and FtATG8f enhanced the antioxidant enzyme activities of TB under adverse stresses. Remarkably, FtATG8a and FtATG8f may be vital candidates functioning in stress resistance in TB. This study prominently aids in understanding the biological role of FtATG genes in TB.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of ATG Gene Family Members in Fagopyrum tataricum and Their Expression during Stress Responses

Fang

Wang

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…The harvest of T3 seeds and the method for hydroponic culture were conducted as described previously ( Huang et al, 2020 ). Three weeks after transferring from the petri dish, plants were divided into two groups and treated with LN (Low N, 0.125 mM NH 4 NO 3 ) and NN (normal N, 2.5 mM NH 4 NO 3 ), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In tea plants, CsATG101 is a single copy ( Huang et al, 2020 ). It was reported that the expression level of CsATG101 changed with abiotic stress, such as drought and cold ( Wang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

CsATG101 Delays Growth and Accelerates Senescence Response to Low Nitrogen Stress in Arabidopsis thaliana

Huang

Hao

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

For tea plants, nitrogen (N) is a foundational element and large quantities of N are required during periods of roundly vigorous growth. However, the fluctuation of N in the tea garden could not always meet the dynamic demand of the tea plants. Autophagy, an intracellular degradation process for materials recycling in eukaryotes, plays an important role in nutrient remobilization upon stressful conditions and leaf senescence. Studies have proven that numerous autophagy-related genes (ATGs) are involved in N utilization efficiency in Arabidopsis thaliana and other species. Here, we identified an ATG gene, CsATG101, and characterized the potential functions in response to N in A. thaliana. The expression patterns of CsATG101 in four categories of aging gradient leaves among 24 tea cultivars indicated that autophagy mainly occurred in mature leaves at a relatively high level. Further, the in planta heterologous expression of CsATG101 in A. thaliana was employed to investigate the response of CsATG101 to low N stress. The results illustrated a delayed transition from vegetative to reproductive growth under normal N conditions, while premature senescence under N deficient conditions in transgenic plants vs. the wild type. The expression profiles of 12 AtATGs confirmed the autophagy process, especially in mature leaves of transgenic plants. Also, the relatively high expression levels for AtAAP1, AtLHT1, AtGLN1;1, and AtNIA1 in mature leaves illustrated that the mature leaves act as the source leaves in transgenic plants. Altogether, the findings demonstrated that CsATG101 is a candidate gene for improving annual fresh tea leaves yield under both deficient and sufficient N conditions via the autophagy process.

show abstract

“…Still, it was possible to detect that CsNRT2 participated in NO 3 − transport under low N conditions ( Hu et al., 2023 ; Lin et al., 2023b ). The AuTophaGy-related genes CsATG8e and CsATG3a were linked to an improved plant ability for N recycling and tolerance to low N levels ( Huang et al., 2020 ; Huang et al., 2023a ). These genes emerge as promising indicators and may contribute to identifying higher NUE among various germplasm resources.…”

Section: An Overview Of Nitrogen Use Efficiency Assessment By Tea Plantsmentioning

confidence: 99%

Nitrogen transport and assimilation in tea plant (Camellia sinensis): a review

Zhang,

Ni,

Long

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Nitrogen is one of the most important nutrients for tea plants, as it contributes significantly to tea yield and serves as the component of amino acids, which in turn affects the quality of tea produced. To achieve higher yields, excessive amounts of N fertilizers mainly in the form of urea have been applied in tea plantations where N fertilizer is prone to convert to nitrate and be lost by leaching in the acid soils. This usually results in elevated costs and environmental pollution. A comprehensive understanding of N metabolism in tea plants and the underlying mechanisms is necessary to identify the key regulators, characterize the functional phenotypes, and finally improve nitrogen use efficiency (NUE). Tea plants absorb and utilize ammonium as the preferred N source, thus a large amount of nitrate remains activated in soils. The improvement of nitrate utilization by tea plants is going to be an alternative aspect for NUE with great potentiality. In the process of N assimilation, nitrate is reduced to ammonium and subsequently derived to the GS-GOGAT pathway, involving the participation of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH). Additionally, theanine, a unique amino acid responsible for umami taste, is biosynthesized by the catalysis of theanine synthetase (TS). In this review, we summarize what is known about the regulation and functioning of the enzymes and transporters implicated in N acquisition and metabolism in tea plants and the current methods for assessing NUE in this species. The challenges and prospects to expand our knowledge on N metabolism and related molecular mechanisms in tea plants which could be a model for woody perennial plant used for vegetative harvest are also discussed to provide the theoretical basis for future research to assess NUE traits more precisely among the vast germplasm resources, thus achieving NUE improvement.

show abstract

Genome-Wide Identification of CsATGs in Tea Plant and the Involvement of CsATG8e in Nitrogen Utilization

Cited by 11 publications

References 60 publications

Genome-Wide Identification of ATG Gene Family Members in Fagopyrum tataricum and Their Expression during Stress Responses

Genome-Wide Identification of ATG Gene Family Members in Fagopyrum tataricum and Their Expression during Stress Responses

CsATG101 Delays Growth and Accelerates Senescence Response to Low Nitrogen Stress in Arabidopsis thaliana

Nitrogen transport and assimilation in tea plant (Camellia sinensis): a review

Contact Info

Product

Resources

About