Multiple FtsZ2 isoforms involved in chloroplast division and biogenesis are developmentally associated with thylakoid membranes in<i>Arabidopsis</i>

Karamoko, Mohamed; El-Kafafi, El-Sayed; Mandaron, Paul; Lerbs-Mache, Silva; Falconet, Denis

doi:10.1016/j.febslet.2011.03.041

Cited by 20 publications

(14 citation statements)

References 29 publications

(46 reference statements)

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“…However, our novel E. coli system provides a new approach for dissecting and uncovering the chloroplast division process in the stromal side, which is topologically equivalent to the bacterial cytosol, although the possible post-translational modifications of AtFtsZs reported by Karamoko et al . 41 would not occur in bacteria.…”

Section: Discussionmentioning

confidence: 95%

ARC6-mediated Z ring-like structure formation of prokaryote-descended chloroplast FtsZ in Escherichia coli

Irieda

Shiomi

2017

Sci Rep

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Plant chloroplasts proliferate through binary fission, and the stromal-side molecules that are involved in chloroplast division are bacterial derivatives. As in bacteria, the prokaryotic tubulin homolog FtsZ assembles into a ring-like structure (Z ring) at mid-chloroplast, and this process is followed by constriction. However, the properties of chloroplast FtsZs remain unclarified. Here, we employed Escherichia coli as a novel heterologous system for expressing chloroplast FtsZs and their regulatory components. Fluorescently labelled Arabidopsis FtsZ2 efficiently assembled into long filaments in E. coli cells, and artificial membrane tethering conferred FtsZ2 filaments with the ability to form Z ring-like structures resembling the bacterial Z ring. A negative regulator of chloroplast FtsZ assembly, ARC3, retained its inhibitory effects on FtsZ2 filamentation and Z ring-like structure formation in E. coli cells. Thus, we provide a novel heterologous system by using bacterial cells to study the regulation of the chloroplast divisome. Furthermore, we demonstrated that the FtsZ2-interacting protein ARC6, which is a potential candidate for Z ring tethering to the chloroplast inner envelope membrane, genuinely targeted FtsZ2 to the membrane components and supported its morphological shift from linear filaments to Z ring-like structures in a manner dependent on the C-terminal ARC6-interacting domain of FtsZ2.

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Section: Discussionmentioning

confidence: 95%

ARC6-mediated Z ring-like structure formation of prokaryote-descended chloroplast FtsZ in Escherichia coli

Irieda

Shiomi

2017

Sci Rep

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“…In that context, sub-plastidial information retrieved from AT_CHLORO was also used to ascertain protein localization. For instance, in a recent paper (Karamoko et al, 2011), AT_CHLORO spectral count-based data reinforced immunoblot analyses showing that two FtsZ2 isoforms were associated with the thylakoid membranes. Another example concerns the CJD1 protein that influences fatty acid composition of chloroplast lipids (Ajjawi et al, 2011).…”

Section: Discussionmentioning

confidence: 82%

AT_CHLORO: A Chloroplast Protein Database Dedicated to Sub-Plastidial Localization

Bruley¹,

Dupierris²,

Salvi³

et al. 2012

Front. Plant Sci.

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AT_CHLORO () is a database dedicated to sub-plastidial localization of A. thaliana chloroplast proteins. This information was infered from proteomics experiments obtained from a comprehensive study that allowed the identification of proteins from envelope, stroma, and thylakoid sub-compartments Ferro et al., 2010. In addition to current knowledge regarding sub-plastidial localization, AT_CHLORO provides experimental data that allowed curated information regarding subcellular localizations of chloroplast proteins to be given. A specific focus was given to proteins that were identified in envelope fractions and for which expert functional annotation was provided. The present mini review shows the specificities of AT_CHLORO with respect to available information, data export options and recent improvements in data representation.

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“…Accordingly, we ask whether thylakoid partitioning accounts for the differences between chloroplast and nongreen plastid division. It has been reported that FtsZ proteins presented both in chloroplast stroma and thylakoid fractions, which suggests they are possibly involved in thylakoid partitioning in dividing chloroplasts [52]. In chloroplast, ARC3 interacts strongly with FtsZ1, and ARC11 interacts with MCD1 and ARC3.…”

Section: Discussionmentioning

confidence: 96%

The Chloroplast Min System Functions Differentially in Two Specific Nongreen Plastids in Arabidopsis thaliana

Wang

Zhang

et al. 2013

PLoS ONE

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The nongreen plastids, such as etioplasts, chromoplasts, etc., as well as chloroplasts, are all derived from proplastids in the meristem. To date, the Min system members in plants have been identified as regulators of FtsZ-ring placement, which are essential for the symmetrical division of chloroplasts. However, the regulation of FtsZ-ring placement in nongreen plastids is poorly understood. In this study, we investigated the division site placement of nongreen plastids by examining the etioplasts as representative in Arabidopsis Min system mutants. Surprisingly, the shape and number of etioplasts in cotyledons of arc3, arc11 and mcd1 mutants were similar to that observed in wild-type plants, whereas arc12 and parc6 mutants exhibited enlarged etioplasts that were reduced in number. In order to examine nongreen plastids in true leaves, we silenced the ALB3 gene in these Min system mutant backgrounds to produce immature chloroplasts without the thylakoidal network using virus induced gene silencing (VIGS). Interestingly, consistent with our observations in etioplasts, enlarged and fewer nongreen plastids were only detected in leaves of parc6 (VIGS-ALB3) and arc12 (VIGS-ALB3) plants. Further, the FtsZ-ring assembled properly at the midpoint in nongreen plastids of arc3, arc11 and mcd1 (VIGS-ALB3) plants, but organized into multiple rings in parc6 (VIGS-ALB3) and presented fragmented filaments in arc12 (VIGS-ALB3) plants, suggesting that division site placement in nongreen plastids requires fewer components of the plant Min system. Taken together, these results suggest that division site placement in nongreen plastids is different from that in chloroplasts.

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Multiple FtsZ2 isoforms involved in chloroplast division and biogenesis are developmentally associated with thylakoid membranes inArabidopsis

Cited by 20 publications

References 29 publications

ARC6-mediated Z ring-like structure formation of prokaryote-descended chloroplast FtsZ in Escherichia coli

ARC6-mediated Z ring-like structure formation of prokaryote-descended chloroplast FtsZ in Escherichia coli

AT_CHLORO: A Chloroplast Protein Database Dedicated to Sub-Plastidial Localization

The Chloroplast Min System Functions Differentially in Two Specific Nongreen Plastids in Arabidopsis thaliana

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