Multiple-frequency impedance measurements in continuous flow for automated evaluation of yeast cell lysis

Mernier, Guillaume; Hasenkamp, Willyan; Piacentini, Niccolò; Renaud, Philippe

doi:10.1016/j.snb.2010.10.050

Cited by 33 publications

(20 citation statements)

References 18 publications

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“…In the low-frequency measurement, the membrane is opaque to the electrical field, most of currents flow through the medium around the cell. When the frequency increases, the current starts pen-etrating the cell and probes the cytosol [34]. The simplified sing-cell model contains a resistor and a capacitor to represent the cytoplasm and the membrane, respectively.…”

Section: Validation Of Proposed Modeling Methodsmentioning

confidence: 99%

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

et al. 2014

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Impedance measurements provide basic electrical properties and are used to analyze the characteristics of electrochemical materials for biomedical applications. The extracellular fluid (ECF) in microfluidic devices greatly affects the accuracy of impedance measurements of cells. When a single cell is placed in large amounts of ECF, the electric current mostly passes through the ECF, not the cell. Hence, this work presents a modeling method that is demonstrated in numerical and analytical solutions for eliminating the effect of ECF in coplanar impedance sensors. The proposed modeling method uses fundamental formulas of circuits that include the electrical parameters of the ECF, cytoplasm, and cell membrane. Equivalent circuit models for the coplanar impedance sensor are established to simulate the impedance as well as the measured ones for excitation frequencies in the range of 11–101 kHz. According to the calculation result using the proposed modeling method, the cytoplasm resistance, membrane capacitance, medium resistance, and medium capacitance of HeLa (human cervix adenocarcinoma) cell are 13.5 kΩ, 122.6 pF, 27.9 kΩ, and 337.7 pF, respectively. Moreover, the electric current distribution in the coplanar impedance sensor is investigated using finite element method (FEM) simulation software. The variation in the impedance during measurements with the simultaneous application of an alternating‐current (AC) voltage amplitude of 0.4 Vpp in the fluid volume range of 9–144 µL is also studied.

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Section: Validation Of Proposed Modeling Methodsmentioning

confidence: 99%

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

et al. 2014

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“…This solution is relevant for many applications, such as cell sorting, impedance spectroscopy and cell lysis [1,3]. We verified that at a flow rate of 5 nL/s, bubbles of 5 µL were successfully removed.…”

Section: First Embodimentmentioning

confidence: 61%

“…Microfluidic devices are used on a daily basis for preparation and/or analysis of biological samples [1][2][3]. To take full profit of the advantages coming from miniaturization, such as small sample volume, samples are injected through tiny channels.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Micro-Debubblers for Bubble Removal with Sub-Microliter Dead Volume

2012

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Abstract:We present the fabrication and evaluation of microdebubblers that are able to remove large bubbles while keeping a very low dead volume. The devices use a polytetrafluoroethylene membrane that is permeable to air in order to filter air bubbles out of an aqueous sample. The dead volume of the devices is less than one microliter, but bubbles as large as 60 microliters can be removed. This simple solution can be very useful for microfluidic devices for chemical or biological analysis that suffer from channel clogging due to the presence of bubbles in their sample. One embodiment is particularly suited for buffer solutions with living cells.

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“…The technique has been used in multiple applications within the field of flow cytometry [5], including applications that involve biological samples, such as white blood cells in different states [5] or bacteria detection and characterization [4,6,7]. Samples that have been counted and separated based on their properties include different type of white blood cells [5], living and dead yeast cells [8], as well as chemo-treated HeLa cells [4]. The technique has also been used in the monitoring of long-term cultivation micro tissue [9].…”

Section: Introductionmentioning

confidence: 99%

Coplanar Electrode Layout Optimized for Increased Sensitivity for Electrical Impedance Spectroscopy

Clausen

Skands²,

Bertelsen

et al. 2014

Micromachines

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Abstract:This work describes an improvement in the layout of coplanar electrodes for electrical impedance spectroscopy. We have developed, fabricated, and tested an improved electrode layout, which improves the sensitivity of an impedance flow cytometry chip. The improved chip was experimentally tested and compared to a chip with a conventional electrode layout. The improved chip was able to discriminate 0.5 μm beads from 1 μm as opposed to the conventional chip. Furthermore, finite element modeling was used to simulate the improvements in electrical field density and uniformity between the electrodes of the new electrode layout. Good agreement was observed between the model and the obtained experimental results.

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Multiple-frequency impedance measurements in continuous flow for automated evaluation of yeast cell lysis

Cited by 33 publications

References 18 publications

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

High-Throughput Micro-Debubblers for Bubble Removal with Sub-Microliter Dead Volume

Coplanar Electrode Layout Optimized for Increased Sensitivity for Electrical Impedance Spectroscopy

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