Multiple frequency dielectrophoresis

Urdaneta, Mario; Smela, Elisabeth

doi:10.1002/elps.200600786

Cited by 71 publications

(75 citation statements)

References 29 publications

(40 reference statements)

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“…This was implemented either by pairs of junction electrodes [28] or interdigitated planar electrodes in the access channel along the main channel that utilized ''liquid electrodes'' to inject current into the main channel [29][30][31]. However, throughput is limited since the height of the channel cannot extend beyond the reach of the electrical field generated from the planar electrodes.…”

Section: Introductionmentioning

confidence: 99%

Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow‐through separation of beads and cells

et al. 2009

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This paper presents a novel design and separation strategy for lateral flow-through separation of cells/particles in microfluidics by dual frequency coupled dielectrophoresis (DEP) forces enabled by vertical interdigitated electrodes embedded in the channel sidewalls. Unlike field-flow-fractionation-DEP separations in microfluidics, which utilize planar electrodes on the microchannel floor to generate a DEP force to balance the gravitational force and separate objects at different height locations, lateral separation is enabled by sidewall interdigitated electrodes that are used to generate non-uniform electric fields and balanced DEP forces along the width of the microchannel. In the current design, two separate AC electric fields are applied to two sets of independent interdigitated electrode arrays fabricated in the sidewalls of the microchannel to generate differential DEP forces that act on the cells/particles flowing through. Individual particles (cells or beads) will experience DEP forces differently due to the difference in their dielectric properties. The balance of the differential DEP forces from the electrode arrays will position dissimilar particles at distinct equilibrium planes across the width of the channel. When coupled with fluid flow, this results in lateral separation along the width of the microchannel and the separated particles can thus be automatically directed into branched channel outlets leading to different reservoirs for downstream processing. In this paper, we present the design and analysis of lateral separation enabled by dual frequency coupled DEP, and cell/bead and cell/cell separations are demonstrated with this lateral separation strategy. With vertical interdigitated electrodes on the sidewall, the height of the microchannel can be increased without losing the electric field strength in contrast to other multiple frequency DEP devices with planar electrodes. As a result, populations of cells can be separated simultaneously instead of one by one to enable high-throughput sorting microfluidic devices.

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Section: Introductionmentioning

confidence: 99%

Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow‐through separation of beads and cells

et al. 2009

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“…(1), and it generally depends on the particle type and AC frequency. These properties have been well studied, enabling the DEP force to be used for concentrating and separating particles, including cells (Urdaneta and Smela 2007;Huang et al 2011;Unni et al 2012;Wu et al 2012). …”

Section: Focusing Of Microparticles By Dielectrophoreticmentioning

confidence: 99%

Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics

Hattori

Kim

Terazono

et al. 2012

Microfluid Nanofluid

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“…Effective concentration and fractionation using iDEP for biological particles in a nondestructive manner has been reported ( Barrett et al 2005;Chen et al 2009;Cho et al 2009;Davalos et al 2008;Kang et al 2009;Kang et al 2008;Lapizco-Encinas et al 2005;Lapizco-Encinas et al 2004a, 2004bMoncadaHernández and Lapizco-Encinas 2010). Differences in cell membrane state and conductivity have an effect on the dielectrophoretic response of cells, allowing viability discrimination; as most cells lose membrane stability as they lose viability (Lapizco-Encinas et al 2004a, 2004bLi and Bashir 2002;Pethig and Markx 1997;Urdaneta and Smela 2007). In general, when a cell losses viability its membrane is compromised and becomes permeable allowing high conductive particles from the cell cytoplasm to penetrate this barrier, which leads to a significant increase of 3-4 orders of magnitude in membrane conductivity (Li and Bashir 2002;Pethig and Markx 1997;Urdaneta and Smela 2007).…”

Section: Introductionmentioning

confidence: 98%

“…Differences in cell membrane state and conductivity have an effect on the dielectrophoretic response of cells, allowing viability discrimination; as most cells lose membrane stability as they lose viability (Lapizco-Encinas et al 2004a, 2004bLi and Bashir 2002;Pethig and Markx 1997;Urdaneta and Smela 2007). In general, when a cell losses viability its membrane is compromised and becomes permeable allowing high conductive particles from the cell cytoplasm to penetrate this barrier, which leads to a significant increase of 3-4 orders of magnitude in membrane conductivity (Li and Bashir 2002;Pethig and Markx 1997;Urdaneta and Smela 2007). Insulator-based DEP can exploit this dielectric difference to selectively trap and, therefore, achieve separation of viable and non-viable cells (Lapizco-Encinas et al 2004a, 2004b.…”

Section: Introductionmentioning

confidence: 99%

Assessment of microalgae viability employing insulator-based dielectrophoresis

Gallo‐Villanueva

Jesús‐Pérez

Martínez-López

et al. 2011

Microfluid Nanofluid

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Microscale bioparticle analysis has advanced significantly providing advantages over bench-scale studies such as the use of a reduced amount of sample and reagents, higher sensitivity, faster response, and portability. Insulator-based dielectrophoresis (iDEP) is a microscale technique where particles are driven by polarization effects under a non-uniform electrical field created by the inclusion of insulators between two electrodes. iDEP possesses attractive advantages over traditional electrode-based dielectrophoresis since there is no electrode degradation and manufacture of the device is simpler and economical. This novel and powerful technique has been applied successfully in the manipulation of macromolecules and cells. In this study, differences in dielectric properties (cell membrane conductivity) of viable and non-viable microalgae, Selenastrum capricornutum, were employed to concentrate and separate a mixture of live and dead cells. A microchannel, manufactured in glass and containing an array of cylindrical insulating posts, was employed to dielectrophoretically immobilize and concentrate the mixture of cells employing direct current electric fields. Experiments showed that live cells exhibited a stronger dielectrophoretic response than dead cells, which allowed cell differentiation. Separation and enrichment of viable and non-viable microalgae was achieved in 35 s with a concentration yield of 10.36 and 15.87 times the initial cell concentration, respectively. These results demonstrate the use of iDEP as a technique for rapid assessment of microalgae viability; unveiling the potential of this powerful technique for environmental applications on lab-on-a-chip platforms.

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Multiple frequency dielectrophoresis

Cited by 71 publications

References 29 publications

Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow‐through separation of beads and cells

Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow‐through separation of beads and cells

Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics

Assessment of microalgae viability employing insulator-based dielectrophoresis

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