Multiple forms of trehalase during development in Dictyostelium discoideum

Killick, Kathleen A.

doi:10.1016/0147-5975(83)90076-2

Cited by 12 publications

(5 citation statements)

References 10 publications

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“…Dictyostelium possesses no hexokinases but structurally unrelated glucokinases (Eichinger et al 25 ; see also http://dictybase.org/), which, of note, showed activity in cells undergoing development. 26 Glucose delayed plasma membrane rupture but not DC-FDA fluorescence, perinuclear clustering of mitochondria or ATP depletion. Thus, the delaying effect of glucose was downstream or independent of early mitochondrial events.…”

Section: Discussionmentioning

confidence: 99%

A necrotic cell death model in a protist

et al. 2006

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While necrotic cell death is attracting considerable interest, its molecular bases are still poorly understood. Investigations in simple biological models, taken for instance outside the animal kingdom, may benefit from less interference from other cell death mechanisms and from better experimental accessibility, while providing phylogenetic information. Can necrotic cell death occur outside the animal kingdom? In the protist Dictyostelium, developmental stimuli induced in an autophagy mutant a stereotyped sequence of events characteristic of necrotic cell death. This sequence included swift mitochondrial uncoupling with mitochondrial 2 0 ,7 0 -dichlorofluorescein diacetate fluorescence, ATP depletion and increased oxygen consumption. This was followed by perinuclear clustering of dilated mitochondria. Rapid plasma membrane rupture then occurred, which was evidenced by time-lapse videos and quantified by FACS. Of additional interest, developmental stimuli and classical mitochondrial uncouplers triggered a similar sequence of events, and exogenous glucose delayed plasma membrane rupture in a nonglycolytic manner. The occurrence of necrotic cell death in the protist Dictyostelium (1) provides a very favorable model for further study of this type of cell death, and (2) strongly suggests that the mechanism underlying necrotic cell death was present in an ancestor common to the Amoebozoa protists and to animals and has been conserved in evolution.

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Section: Discussionmentioning

confidence: 99%

A necrotic cell death model in a protist

et al. 2006

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“…Commitment to aggregation is enough to induce trehalase secretion since aggregation itself can be inhibited without preventing the secretion of trehalase (24). During formation of the fruiting bodies, trehalase activity increases again and reaches a value of between 50 and 200% of the value found in free-living myxamoebae (83,84,152). The increase in the activity is restricted completely to the stalk cells of the fruiting body.…”

Section: Nonregulatory and Regulatory Trehalasesmentioning

confidence: 99%

“…The trehalase activity remains approximately constant in free-living myxamoebae (24). Aggregation of the vegetative myxamoebae upon starvation is associated with secretion of trehalase into the medium, which results in a drastic decrease in the intracellular activity (24,83,84,152). Commitment to aggregation is enough to induce trehalase secretion since aggregation itself can be inhibited without preventing the secretion of trehalase (24).…”

Section: Nonregulatory and Regulatory Trehalasesmentioning

confidence: 99%

Regulation of trehalose mobilization in fungi

Thevelein¹

1984

Microbiol Rev

285

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“…Coincident with alterations in trehalase solubility is the appearance of multiple forms of the enzyme during terminal differentiation in D. discoideum (Killick, 1983). A single major enzyme activity, isoenzyme I, is detectable prior to the 12th hour of development, but during culmination a second electrophoretically distinct form of trehalase (isoenzyme 11) accumulates, and at least 70% of the soluble trehalase activity prepared during culmination is this isoenzyme (Killick, 198 1 a).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, evidence has been presented which indicates that extracts from mature fruits of Dictyostelium contain electrophoretically distinct multiple trehalase activities and that these isoenzymes are under developmental regulation (Killick, 1981 a, b;1983). Thus, although a single intracellular trehalase activity (isoenzyme I) was detectable prior to 14 h of development, during culmination a second isoenzyme (isoenzyme 11) accumulated.…”

Section: Introductionmentioning

confidence: 99%

Alterations in Trehalase Solubility during Development in the Cellular Slime Mould Dictyostelium discoideum

Killick¹

1985

Microbiology

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Previous studies have indicated that during development in the slime mould Dictyostelium discoideum, compartmentation of the isoenzymes of trehalase (alpha, alpha'-trehalose 1-D-glucohydrolase, (EC 3.2.1.28) occurs between the extracellular and intracellular environments. The compartmentation of trehalase between soluble and particulate cell fractions was examined in this work. The trehalase present in crude homogenates prepared during the first 12 h of development was completely soluble. Starting at about the pseudoplasmodial stage (i.e. the 14th hour of development), trehalase activity became associated with insoluble cellular material and this increased to a maximal value in homogenates from mature sorocarps, where 50% of the activity was insoluble. Spore cells accounted for only 2 to 3% of the trehalase associated with mature sorocarps, with the remaining 97% being localized in stalk cell material. Although trehalase recovered from spores was completely soluble, more than half of that from the stalk was recovered in the buffer-insoluble pellet fraction.

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Multiple forms of trehalase during development in Dictyostelium discoideum

Cited by 12 publications

References 10 publications

A necrotic cell death model in a protist

A necrotic cell death model in a protist

Regulation of trehalose mobilization in fungi

Alterations in Trehalase Solubility during Development in the Cellular Slime Mould Dictyostelium discoideum

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