Regulation of trehalose mobilization in fungi

Thevelein, Johan M.

doi:10.1128/mr.48.1.42-59.1984

Cited by 290 publications

(42 citation statements)

References 204 publications

(337 reference statements)

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“…and it has been proposed that this is attributable to Dcs1dependent inhibition at the protein level (39). In this context it is notable that Thevelein previously suggested that cAMPdependent PKA-mediated phosphorylation is involved in the regulation of Nth1 activity (43), leading to the proposal that phosphorylation might be used to modulate an interaction between Dcs1 and Nth1 (39). However, we have found no evidence of direct regulation of trehalase activity by Dcs1, irrespective of the phosphorylation state of the latter protein.…”

Section: Discussioncontrasting

confidence: 66%

The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast

Malys¹

2004

Nucleic Acids Research

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Dcs1, the m7GpppX pyrophosphatase of Saccharomyces cerevisiae, has been reported to 'scavenge' capped 5' end fragments generated by 3'-->5' mRNA degradation. We now show that the absence of Dcs1, and the closely related Dcs2 protein, compromises cellular responses to glucose-deprivation stress as well as to step changes in glucose availability. Dcs1 and Dcs2 form homo- and heterodimers, with the heterodimer appearing as cells enter diauxie. Despite the previously observed increase in abundance of the mRNA encoding the neutral trehalase (Nth1) in the stationary phase, the total enzyme activity of Nth1 decreases in this phase of growth. Changes in trehalase activity are significant because the non-reducing disaccharide trehalose is thought to stabilize cellular components under stress conditions. In the dcs1Delta and dcs1Deltadcs2Delta mutants, normal regulation of trehalase activity is lost. Nutrient stress induces DCS1 and DCS2 transcription via the cAMP-PKA signalling pathway. Dcs1 also becomes phosphorylated as the availability of glucose diminishes, and we test the role of this phosphorylation in the stress response. Further evidence indicates that Dcs1 plays a complementary role to the translation factor eIF4E in preventing capped 5' fragments of mRNA from interfering with translation initiation. We conclude that Dcs1 function influences cellular responses to changes in nutrient avialability, while Dcs2 seems to act as a modulator of Dcs1 function.

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Section: Discussioncontrasting

confidence: 66%

The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast

Malys¹

2004

Nucleic Acids Research

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“…Trehalose is suggested to function as a reserve carbohydrate, as an osmo-protectant, or for protection against protein denaturation by dehydration [81,82]. For example, spores that have a high content of trehalose are more resistant to temperature stress, dehydration, freezing, oxidizing agents, or starvation [81,[83][84][85][86]. A. nidulans and S. cerevisiae mutants genes are also involved in the regulation of cell wall structure by modulating chitin synthase activity [88][89][90][91], hence the effect of these genes might intertwine both physiological responses.…”

Section: Transcriptional Profiling Of Genes Coding For Osmolyte Synthesis Enzymesmentioning

confidence: 99%

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Rodríguez-Pupo

Pérez-Llano

Tinoco-Valencia

et al. 2021

JoF

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Aspergillus sydowii is a moderate halophile fungus extensively studied for its biotechnological potential and halophile responses, which has also been reported as a coral reef pathogen. In a recent publication, the transcriptomic analysis of this fungus, when growing on wheat straw, showed that genes related to cell wall modification and cation transporters were upregulated under hypersaline conditions but not under 0.5 M NaCl, the optimal salinity for growth in this strain. This led us to study osmolyte accumulation as a mechanism to withstand moderate salinity. In this work, we show that A. sydowii accumulates trehalose, arabitol, mannitol, and glycerol with different temporal dynamics, which depend on whether the fungus is exposed to hypo- or hyperosmotic stress. The transcripts coding for enzymes responsible for polyalcohol synthesis were regulated in a stress-dependent manner. Interestingly, A. sydowii contains three homologs (Hog1, Hog2 and MpkC) of the Hog1 MAPK, the master regulator of hyperosmotic stress response in S. cerevisiae and other fungi. We show a differential regulation of these MAPKs under different salinity conditions, including sustained basal Hog1/Hog2 phosphorylation levels in the absence of NaCl or in the presence of 2.0 M NaCl, in contrast to what is observed in S. cerevisiae. These findings indicate that halophilic fungi such as A. sydowii utilize different osmoadaptation mechanisms to hypersaline conditions.

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“…The most widely distributed trehalose biosynthesis pathway contains two key enzymes—trehalose 6-phosphate synthase (Tps1) and trehalose 6-phosphate phosphatase (Tps2)—in a complex called the TPS complex ( 7 , 67 ). In yeast, the TPS complex contains two regulatory subunits, named Tps3p and Tsl1p.…”

Section: Discussionmentioning

confidence: 99%

Trehalose Phosphate Synthase Complex-Mediated Regulation of Trehalose 6-Phosphate Homeostasis Is Critical for Development and Pathogenesis in Magnaporthe oryzae

et al. 2021

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Regulation of trehalose mobilization in fungi

Cited by 290 publications

References 204 publications

The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast

The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Trehalose Phosphate Synthase Complex-Mediated Regulation of Trehalose 6-Phosphate Homeostasis Is Critical for Development and Pathogenesis in Magnaporthe oryzae

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