Multiple forms of prostate-specific antigen in serum measured differently in equimolar- and skewed-response assays

Strobel, St.; Sokoloff, Roger L.; Wolfert, Robert L.; Rittenhouse, Harry G.

doi:10.1093/clinchem/41.1.125

Cited by 16 publications

(1 citation statement)

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“…It has recently been reported that some commercial PSA assays that employ a monoclonal antibody (capture)-polyclonal antibody (tag) format do not measure free and ACTcomplexed PSA on an equimolar basis (13, [25][26][27]. Stamey (19) prepared in vitro mixtures of PSA-ACT, containing 0-100% of complexed PSA, in which the total PSA concentration of each mixture remained constant.…”

Section: Discussionmentioning

confidence: 99%

Technicon Immuno 1® PSA assay measures both free and alpha-1-antichymotrypsin-complexed prostate-specific antigen on an equimolar basis

Zhou

Very

et al. 1996

J. Clin. Lab. Anal.

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In this study, we investigated the immunoreactivity of the Technicon Immuno 1® PSA assay, a monoclonal‐polyclonal sandwich immunoassay, with free and ACT‐complexed PSA. Assay calibrators prepared with free PSA (standard immuno 1 calibrators) and calibrators consisting of 90% ACT‐complexed and 10% free PSA (90:10 calibrators) yielded virtually identical PSA recoveries at all concentrations tested. Concentrations of total PSA at ∼4 and 10 ng/mL, prepared in varying ratios of free PSA to PSA‐ACT complex, recovered from 92–104% at 4 ng/mL and from 98–102% at 10 ng/mL. Additionally, an excellent correlation of serum total PSA values from a panel of 40 prostatic cancer patient samples was obtained using calibration curves generated with the standard Immuno 1 calibrators or the 90:10 calibrators. These results demonstrate that the Technicon Immuno 1® PSA assay measures free and ACT‐complexed PSA on an equal molar basis. © 1996 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Technicon Immuno 1® PSA assay measures both free and alpha-1-antichymotrypsin-complexed prostate-specific antigen on an equimolar basis

Zhou

Very

et al. 1996

J. Clin. Lab. Anal.

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Dual monoclonal antibody immunoassay for free prostate-specific antigen

Wang¹,

Hill²,

Sokoloff³

et al. 1996

Prostate

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Prostate‐specific antigen (PSA) is the most important tumor marker for early detection and monitoring of prostate cancer (PCa) patients. PSA is also elevated in many patients with benign prostatic hyperplasia (BPH). The study of the serum PSA forms, free PSA (f‐PSA) and PSA complexed with α1‐antichymotrypsin (PSA‐ACT), may improve the discrimination between PCa and BPH. An immunoassay specific for f‐PSA is reported with very low cross‐reactivity (0.7%) to PSA‐ACT. Serum specimens from BPH and PCa patients (determined by biopsy) with PSA levels from <1 to >100 ng/ml were tested. No f‐PSA was detected in serum specimens from normal females (N = 50). Low levels (0–0.3 ng/ml) were detected in specimens from healthy males (N = 60). In specimens from PCa and BPH patients, the f‐PSA to total PSA ratio (f/t) was found to range from 1% to higher than 60%. While maintaining an 80% sensitivity for cancer, the f/t ratio improved specificity to approximately 80%, as compared to 55% for total PSA alone. The receiver operating characteristics (ROC) curve analysis of the f/t ratio displayed a greater area under the plot (0.84) compared to total PSA alone (0.745). The results demonstrate that the f/t ratio significantly increases specificity for PCa detection compared to total PSA alone, showing the potential clinical value of the f‐PSA immunoassay. © 1996 Wiley‐Liss, Inc.

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Methods for the measurement of benzodiazepines in biological samples

Drummer

1998

Journal of Chromatography B: Biomedical Sciences and Applicatio

121

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Multiple forms of prostate-specific antigen in serum measured differently in equimolar- and skewed-response assays

Cited by 16 publications

References 0 publications

Technicon Immuno 1® PSA assay measures both free and alpha-1-antichymotrypsin-complexed prostate-specific antigen on an equimolar basis

Technicon Immuno 1® PSA assay measures both free and alpha-1-antichymotrypsin-complexed prostate-specific antigen on an equimolar basis

Dual monoclonal antibody immunoassay for free prostate-specific antigen

Methods for the measurement of benzodiazepines in biological samples

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