Multiple ESBL-Producing Escherichia coli Sequence Types Carrying Quinolone and Aminoglycoside Resistance Genes Circulating in Companion and Domestic Farm Animals in Mwanza, Tanzania, Harbor Commonly Occurring Plasmids

Seni, Jeremiah; Falgenhauer, Linda; Simeo, Nabina; Mirambo, Mariam M.; Imirzalioglu, Can; Matee, Mecky; Rweyemamu, Mark M.; Chakraborty, Trinad; Mshana, Stephen E.

doi:10.3389/fmicb.2016.00142

Cited by 71 publications

(102 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The virulence and resistant genes found in APEC have been detected on IncFIB plasmids, such as pAPEC-1 (Mellata et al, 2012). All plasmid replicons reported in the current study have been previously detected in E. coli isolates sourced from the environment, other animals and humans (Bortolaia et al, 2010;Garcia-Fernandez et al, 2008;Kluytmans et al, 2013;Lynne et al, 2012;Seni et al, 2016;Wang et al, 2013a;Woodford et al, 2007).…”

Section: Investigating the Prevalence Of Carriage Of Avian Pathogenicsupporting

confidence: 53%

“…The IncI1, IncFIB, IncY and IncN plasmids have been identified in poultry previously and were associated with MDR and ESBLs producing E. coli (Bortolaia et al, 2010;Garcia-Fernandez et al, 2008;Kluytmans et al, 2013;Lynne et al, 2012;Seni et al, 2016;Wang et al, 2013a). One of the ESC resistant isolates contained IncY and IncI1 plasmid types, while the other contained IncFIA, IncFIB and IncFrep plasmids.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

show abstract

Section: Investigating the Prevalence Of Carriage Of Avian Pathogenicsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 96%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

View full text Add to dashboard Cite

show abstract

“…Previous studies have reported on the coexistence of carbapenemases, ESBLs, and AMEs in Enterobacteriaceae (29)(30)(31)(32)(33)(34). Genes encoding AMEs have been found on the same plasmids and integrons as genes encoding KPC or ESBLs (35)(36)(37)(38)(39). Less commonly, KPC and ESBL genes may coexist on the same plasmids (37,40).…”

Section: Discussionmentioning

confidence: 99%

Association between the Presence of Aminoglycoside-Modifying Enzymes and In Vitro Activity of Gentamicin, Tobramycin, Amikacin, and Plazomicin against Klebsiella pneumoniae Carbapenemase- and Extended-Spectrum-β-Lactamase-Producing Enterobacter Species

Haidar

Alkroud

Cheng

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

We compared the in vitro activities of gentamicin (GEN), tobramycin (TOB), amikacin (AMK), and plazomicin (PLZ) against 13Enterobacter isolates possessing both Klebsiella pneumoniae carbapenemase and extended-spectrum ␤-lactamase (KPC؉/ ESBL؉) with activity against 8 KPC؉/ESBL؊, 6 KPC؊/ESBL؉, and 38 KPC؊/ESBL؊ isolates. The rates of resistance to GEN and TOB were higher for KPC؉/ESBL؉ (100% for both) than for KPC؉/ESBL؊ (25% and 38%, respectively), KPC؊/ESBL؉ (50% and 17%, respectively), and KPC؊/ESBL؊ (0% and 3%, respectively) isolates. KPC؉/ESBL؉ isolates were more likely than others to possess an aminoglycoside-modifying enzyme (AME) (100% versus 38%, 67%, and 5%; P ‫؍‬ 0.007, 0.06, and <0.0001, respectively) or multiple AMEs (100% versus 13%, 33%, and 0%, respectively; P < 0.01 for all). KPC؉/ESBL؉ isolates also had a greater number of AMEs (mean of 4.6 versus 1.5, 0.9, and 0.05, respectively; P < 0.01 for all). GEN and TOB MICs were higher against isolates with >1 AME than with <1 AME. The presence of at least 2/3 of KPC, SHV, and TEM predicted the presence of AMEs. PLZ MICs against all isolates were <4 g/ml, regardless of KPC/ESBL pattern or the presence of AMEs. In conclusion, GEN and TOB are limited as treatment options against KPC؉ and ESBL؉ Enterobacter. PLZ may represent a valuable addition to the antimicrobial armamentarium. A full understanding of AMEs and other aminoglycoside resistance mechanisms will allow clinicians to incorporate PLZ rationally into treatment regimens. The development of molecular assays that accurately and rapidly predict antimicrobial responses among KPC-and ESBL-producing Enterobacter spp. should be a top research priority. Enterobacter aerogenes and Enterobacter cloacae are important nosocomial pathogens (1), which collectively constitute the 8th most common cause of health care-associated infections (2). Enterobacter spp. are well recognized for their capacity to develop acquired ␤-lactam resistance via inducible or derepressed production of AmpC ␤-lactamases (3, 4). In addition, Enterobacter isolates often manifest multiple antibiotic resistance mechanisms, including coproduction of extended-spectrum ␤-lactamases (ESBLs), upregulation of efflux pumps, and deficiency of outer membrane porins, which may confer multidrug resistance (MDR) phenotypes (5-8). More recently, carbapenem-resistant Enterobacter spp. have emerged worldwide (6, 9-15). As with other carbapenem-resistant Enterobacteriaceae (CRE), carbapenem resistance in Enterobacter spp. is most commonly conferred through production of Klebsiella pneumoniae carbapenemases (KPCs) or metallo-␤-lactamases (MBLs) (6, 16). These resistance determinants are commonly located on plasmids that carry other genes attenuating susceptibility to multiple antibiotic classes. As a result, therapeutic options against carbapenem-resistant Enterobacter infections are limited, and optimal treatment regimens are yet to be established.Aminoglycosides retain potent bactericidal activity against some, but not all, CRE (17). The most common de...

show abstract

“…Studies performed in this hospital have shown high rates of ESBL-producing K. pneumoniae and E. coli carrying bla CTX-M-15 in IncF plasmids (Mshana et al, 2011, 2013). In addition recent studies involving isolates from animals and humans from community have shown the bla CTX-M-15 allele to be predominant (Mshana et al, 2016; Seni et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

“…Evidence in the literature has also documented the probable horizontal transfer of resistance genes from either human sewage or clinical isolates to fish in rivers or lakes in which drainage of wastewater from treatment plants occurs (Kümmerer, 2009; Martinez, 2009b; Jiang et al, 2012; Blaak et al, 2014). Multiple genotypes of ESBL-producing E. coli have been found in animals and humans in Mwanza (Mshana et al, 2011, 2016; Seni et al, 2016). There are few studies from developed countries on the presence of ESBL-producing isolates in fish and environment samples and the role played by the food chain in transmission of resistance genes through contamination by human and animals sewage and anthropogenic activities in relation to water bodies (Zurfluh et al, 2013; Abgottspon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania

et al. 2016

Self Cite

View full text Add to dashboard Cite

Extended-Spectrum Beta-Lactamase (ESBL)-producing bacteria are a common cause of healthcare and community-associated infections worldwide. The distribution of such isolates in the environment and their presence in fish as a result of sewage contamination is not well-studied. Here we examined fish and environmental samples from Mwanza city for the presence of ESBL-producing bacteria. From 196 fish sampled from local markets, 26 (13.3%) contained lactose-fermenting ESBL-producing bacteria, while 39/73 (53.4%) environmental samples from the same area were ESBL producers. Antibiotic resistance genes, multi locus sequence types (MLST) and plasmid replicon types in 24 selected isolates from both populations were identified with whole genome sequencing using Illumina MiSeq. Nine of eleven sequenced fish isolates had the blaCTX-M-15 gene whereas 12/13 from environment carried blaCTX-M-15. Antibiotic resistance genes encoding resistance to sulfonamides (sul1/sul2), tetracyclines [tet(A)/tet(B)] fluoroquinolones [e.g., aac(6′)-Ib-cr, qnrS1], aminoglycosides [e.g., aac(3)-lld, strB, strA,] and trimethoprim (e.g., dfrA14) were detected. E. coli sequence type ST-38 (2) and ST-5173 (2) were detected in isolates both from the environment and fish. IncY plasmids carrying blaCTX-M-15, qnrS1, strA, and strB were detected in five environmental E. coli isolates and in one E. coli isolate from fish. Our data indicate spillage of resistant environmental isolates into Lake Victoria through the sewage system. Persistence of blaCTX-M-15 in the Mwanza city environment is complex, and involves both clonal spread of resistant strains as well as dissemination by commonly occurring IncY plasmids circulating in isolates present in humans, the environment as well as in the food chain.

show abstract

Multiple ESBL-Producing Escherichia coli Sequence Types Carrying Quinolone and Aminoglycoside Resistance Genes Circulating in Companion and Domestic Farm Animals in Mwanza, Tanzania, Harbor Commonly Occurring Plasmids

Cited by 71 publications

References 34 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Association between the Presence of Aminoglycoside-Modifying Enzymes and In Vitro Activity of Gentamicin, Tobramycin, Amikacin, and Plazomicin against Klebsiella pneumoniae Carbapenemase- and Extended-Spectrum-β-Lactamase-Producing Enterobacter Species

Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania

Contact Info

Product

Resources

About