Multiple effects of silicon on alleviation of nickel toxicity in young maize roots

Vaculík, Marek; Kováč, Ján; Fialová, Ivana; Fiala, Roderik; Jašková, Katarína; Luxová, Miroslava

doi:10.1016/j.jhazmat.2021.125570

Cited by 19 publications

(6 citation statements)

References 59 publications

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“…Regarding root length, there is little differentiation between treatments (with and without AMF) and both obtained positive results in relation to root system growth, evidencing possible action of Si in increasing the tolerance of plants in the presence of Mn. However, the mechanisms of Si responsible for this possible protection are still not well understood (VACULÍK et al, 2021). Ur Rahman et al (2021) state that Si supplementation in wheat plants grown under cadmium stress increased the length and volume of their roots.…”

Section: Discussionmentioning

confidence: 99%

“…The decrease in leaf toxicity percentage in these plants may probably be associated with a decrease in the availability of this metal in the soil and, consequently, its lower absorption. Some authors recognize the action of Si in the immobilization of HM in the soil (VACULÍK et al, 2021). Bhat et al (2019) explain that Si can stimulate plant roots to release a greater amount of flavonoids and organic acids that can act in the chelation of metals in the soil and, therefore, reduce their phytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

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Silicon Application and Mycorrhiza Inoculation Promoted Leucaena Leucocephala Growth in a Soil Highly Contaminated by Manganese

Carmo

Garcia

Filho

et al. 2022

NAT

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Arbuscular mycorrhizal fungi (AMF) can increase the acquisition of silicon (Si) and, therefore, alleviate the problems caused by metallic toxicity in plants, but this effect remains poorly understood. The objective was to evaluate the influence of Si application on the growth of Leucaena leucocephala inoculated with AMF (Claroideoglomus etunicatum) in a soil contaminated by manganese (Mn). We exposed plants to increasing levels of Si (0, 100, 200 and 400 mg kg-1) in the soil for 90 days. Intermediate levels of Si and AMF inoculation significantly increased shoot and root dry mass, the number of sheets, root system length and mycorrhizal colonization. The abundance of AMF spores decreased linearly with increasing levels of Si applied to the soil, suggesting a low correlation with mycorrhizal colonization. In addition to the higher Mn contents in the shoots and, mainly, in the roots, the combined application of Si and inoculation with AMF significantly reduced foliar toxicity by more than 40%, when compared to the absence of Si and AMF inoculation. Our results demonstrated a synergistic effect of AMF and Si in improving the growth and tolerance of L. leucocephala plants in soil contaminated by Mn. Keywords: heavy metals; stress; remediation; AMF; Mn toxicity. Aplicação de silício e inoculação de micorriza promove crescimento de Leucaena leucocephala em solo altamente contaminado por manganês RESUMO: Os fungos micorrízicos arbusculares (FMA) podem aumentar a aquisição de silício (Si) e, portanto, amenizar os problemas causados pela toxidez metálica nas plantas, mas esse efeito ainda é pouco conhecido. O objetivo foi avaliar a influência da aplicação de Si no crescimento de Leucaena leucocephala inoculada com FMA (Claroideoglomus etunicatum) em solo contaminado por manganês (Mn). Expusemos as plantas a níveis crescentes de Si (0, 100, 200 e 400 mg kg-1) no solo por 90 dias. Níveis intermediários de Si e inoculação de FMA aumentaram significativamente a massa seca da parte aérea e radicular, o número de folhas, o comprimento do sistema radicular e a colonização micorrízica. A abundância de esporos de FMA diminuiu linearmente com o aumento dos níveis de Si aplicados ao solo, sugerindo uma baixa correlação com a colonização micorrízica. Além dos maiores teores de Mn na parte aérea e, principalmente, nas raízes, a aplicação combinada de Si e inoculação com FMA reduziu significativamente a toxidez foliar em mais de 40%, quando comparada à ausência de Si e inoculação de FMA. Nossos resultados demonstraram um efeito sinérgico de FMA e Si na melhoria do crescimento e tolerância de plantas de L. leucocephala em solo contaminado por Mn. Palavras-chave: metais pesados; estresse; remediação; FMA; toxidez por Mn.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Silicon Application and Mycorrhiza Inoculation Promoted Leucaena Leucocephala Growth in a Soil Highly Contaminated by Manganese

Carmo

Garcia

Filho

et al. 2022

NAT

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“…The previously described method for extracting antioxidant enzymes from fresh soybean sample was followed ( Vaculík et al., 2021 ). First, powders of the samples were mixed with sodium phosphate buffer (1 mM EDTA), filtered, and centrifuged to obtain the supernatant.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of GmWRKY172 enhances cadmium tolerance in plants and reduces cadmium accumulation in soybean seeds

et al. 2023

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IntroductionCadmium (Cd) stress is a significant threat to soybean production, and enhancing Cd tolerance in soybean is the focus of this study. The WRKY transcription factor family is associated with abiotic stress response processes. In this study, we aimed to identify a Cd-responsive WRKY transcription factor GmWRKY172 from soybean and investigate its potential for enhancing Cd tolerance in soybean.MethodsThe characterization of GmWRKY172 involved analyzing its expression pattern, subcellular localization, and transcriptional activity. To assess the impact of GmWRKY172, transgenic Arabidopsis and soybean plants were generated and examined for their tolerance to Cd and Cd content in shoots. Additionally, transgenic soybean plants were evaluated for Cd translocation and various physiological stress indicators. RNA sequencing was performed to identify the potential biological pathways regulated by GmWRKY172.ResultsGmWRKY172 was significantly upregulated by Cd stress, highly expressed in leaves and flowers, and localized to the nucleus with transcriptional activity. Transgenic plants overexpressing GmWRKY172 showed enhanced Cd tolerance and reduced Cd content in shoots compared to WT. Lower Cd translocation from roots to shoots and seeds was also observed in transgenic soybean. Under Cd stress, transgenic soybean accumulated less malondialdehyde (MDA) and hydrogen peroxide (H2O2) than WT plants, with higher flavonoid and lignin contents, and peroxidase (POD) activity. RNA sequencing analysis revealed that many stress-related pathways were regulated by GmWRKY172 in transgenic soybean, including flavonoid biosynthesis, cell wall synthesis, and peroxidase activity.DiscussionOur findings demonstrated that GmWRKY172 enhances Cd tolerance and reduces seed Cd accumulation in soybean by regulating multiple stress-related pathways, and could be a promising candidate for breeding Cd-tolerant and low Cd soybean varieties.

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“…Similarly, Farooq et al ( 2013) also confirmed that Si treatment helped to maintain the normalization of cell membrane. Toxicity of Cd is also usually manifested by overproduction of toxic ROS (Vaculıḱ et al, 2021). Previous research reported that excessive ROS accumulated in plants under Cd stress was able to lead to plant growth retardation (Zhou and Qiu, 2005), inhibition of photosynthetic pigment synthesis (Wahid et al, 2008;Farooq et al, 2013), cell wall looseness during cell expansion (Berni et al, 2019), and enzyme inactivation (Farooq et al, 2013).…”

Section: Exogenous Si Alleviated the CD Toxicity Of S Alfrediimentioning

confidence: 99%

Exogenous silicon promotes cadmium (Cd) accumulation in Sedum alfredii Hance by enhancing Cd uptake and alleviating Cd toxicity

Zhou²,

Shi³

et al. 2023

Front. Plant Sci.

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Soil Cadmium (Cd) pollution has become a serious environmental problem. Silicon (Si) plays key roles in alleviating Cd toxicity in plants. However, the effects of Si on mitigation of Cd toxicity and accumulation of Cd by hyperaccumulators are largely unknown. This study was conducted to investigate the effect of Si on Cd accumulation and the physiological characteristics of Cd hyperaccumulator Sedum alfredii Hance under Cd stress. Results showed that, exogenous Si application promoted the biomass, Cd translocation and concentration of S. alfredii, with an increased rate of 21.74-52.17% for shoot biomass, and 412.39-621.00% for Cd accumulation. Moreover, Si alleviated Cd toxicity by: (i) increasing chlorophyll contents, (ii) improving antioxidant enzymes, (iii) enhancing cell wall components (lignin, cellulose, hemicellulose and pectin), (iv) raising the secretion of organic acids (oxalic acid, tartaric acid and L-malic acid). The RT-PCR analysis of genes that involved in Cd detoxification showed that the expression of SaNramp3, SaNramp6, SaHMA2 and SaHMA4 in roots were significantly decreased by 11.46-28.23%, 6.61-65.19%, 38.47-80.87%, 44.80-69.85% and 33.96-71.70% in the Si treatments, while Si significantly increased the expression of SaCAD. This study expanded understanding on the role of Si in phytoextraction and provided a feasible strategy for assisting phytoextraction Cd by S. alfredii. In summary, Si facilitated the Cd phytoextraction of S. alfredii by promoting plant growth and enhancing the resistance of plants to Cd.

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Multiple effects of silicon on alleviation of nickel toxicity in young maize roots

Cited by 19 publications

References 59 publications

Silicon Application and Mycorrhiza Inoculation Promoted Leucaena Leucocephala Growth in a Soil Highly Contaminated by Manganese

Silicon Application and Mycorrhiza Inoculation Promoted Leucaena Leucocephala Growth in a Soil Highly Contaminated by Manganese

Overexpression of GmWRKY172 enhances cadmium tolerance in plants and reduces cadmium accumulation in soybean seeds

Exogenous silicon promotes cadmium (Cd) accumulation in Sedum alfredii Hance by enhancing Cd uptake and alleviating Cd toxicity

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