Multiple bud cultures of ‘Barhee’ date palm (Phoenix dactylifera) and physiological status of regenerated plants

Fki, Lotfi; Bouaziz, Neila; Kriaâ, Walid; Benjemaa-Masmoudi, Raja; Gargouri-Bouzid, Radhia; Rival, Alain; Drira, Noureddine

doi:10.1016/j.jplph.2011.03.013

Cited by 38 publications

(22 citation statements)

References 42 publications

(34 reference statements)

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“…However, very few commercial tissue culture laboratories are now able to provide sizeable quantities of plants because of the known recalcitrance of this species to in vitro regeneration (Drira et al 1985). In fact, date palm tissue cultures grow very slowly: the initiation phase may require more than 24 months, especially when low amounts of plant growth regulators are used in order to avoid somaclonal variation (Cohen et al 2011, Fki et al 2011a. The presence of uncontrollable endophytic bacteria is another serious constraint hampering the large scale micropropagation of date palm (Fki et al 2010).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

“…Khenizi shoots were established in our laboratory according to our previously described protocol (Fki et al 2011a) which is based on the utilization of low concentration of 2,4-D (0.1 mg dm -3 ) with fragmented juvenile leafs excised from offshoots as primary explants. Hypertrophied achlorophyllous leaves were excised from shoot cultures, cut in 4 mm size pieces and cultivated onto solid culture medium for the initiation of adventitious meristems.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

“…1A). Our previous studies showed that plant growth regulators (PGRs) such as 2,4-D were essential to induce organogenesis on primary explants in date palm and that the culture period required to observe the first adventitious buds was much longer (17 months), especially when low concentrations of 2,4-D were used (Fki et al 2011a). Removing PGRs from culture media can minimise the risk of both somaclonal variation and loss of morphogenetic capacity (Bairu et al 2011).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

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Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Fki¹,

Bouaziz²,

Chkir³

et al. 2013

Biologia plant.

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Date palm (Phoenix dactylifera L.) cv. Khenizi caulogenic meristems were initiated from achlorophyllous leaves excised from in vitro shoot cultures and then proliferated on a specific culture medium supplemented with 70 g dm-3 sucrose. Regeneration rates obtained when using standard vitrification, droplet-vitrification, and encapsulationvitrification protocols reached 26.7, 60.0, and 40.0 %, respectively. Only explants smaller than 3 mm in diameter were found to survive cryogenic treatments. Sucrose preculture, cold hardening and loading solution pretreatments showed significant effects on regeneration rates. Moreover, our results indicate that both sucrose preculture and cold acclimation of explants increased proline content. Cryopreservation of date palm tissue with high proliferation capacity can directly benefit large scale micropropagation projects. (Résumé d'auteur

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

See 1 more Smart Citation

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Fki¹,

Bouaziz²,

Chkir³

et al. 2013

Biologia plant.

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“…Hillawi, which resulted in an average of 18.2 buds per culture [46]. Other factors such as the medium texture [54,55], cultivation in bioreactors [56], explant size and density [57] and carbon source [56,58,59] were also reported to affect shoot bud multiplication of date palm.…”

Section: Shoot Bud Multiplicationmentioning

confidence: 99%

Micropropagation of Date Palm: A Review

Mazri¹,

Meziani²

2015

Cell Dev Biol

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Somatic embryogenesis and organogenesis are the two pathways of choice for rapid and large-scale propagation of date palm. They have been successfully used for the micropropagation of elite genotypes and have proved their effectiveness for the commercial production of many cultivars. Nevertheless, regeneration through somatic embryogenesis or organogenesis in date palm is still difficult to achieve for recalcitrant genotypes and is often hampered by certain physiological disorders. In the present review, we report the results of a number of studies carried out on date palm micropropagation. It also describes different factors that influence each stage of somatic embryogenesis and organogenesis and the main problems encountered during these two micropropagation processes.

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“…'Maktoom' (Khierallah and Bader, 2007), cv. 'Barhee' (Fki et al, 2011) andcv. 'Khanezi' (Al-Khateeb, 2006;.…”

Section: Effect Of Explants Size On Shoot Length and Rootingmentioning

confidence: 99%

Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

Mazri¹

2013

Not Sci Biol

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The effect of basal medium, explant size and density on shoot multiplication, growth, rooting and acclimatization of date palm cv. '16-bis' was evaluated. Bud clusters of different sizes (2, 3, 4 and 5 buds per cluster) were cultured at density of 1, 2, 3 and 4 clusters on Murashige and Skoog medium (MS), woody plant medium (WPM) and Nitsch medium (NM) supplemented with 0.5 mg/L 2-naphthoxyacetic acid and 0.5 mg/L kinetin for three months (multiplication phase). Separated shoots of different sizes (<3 cm; 3 to 4.5 cm and 4.5 to 6 cm) were cultured at density of 1, 2, 3 and 4 shoots on hormone free MS medium, WPM or NM for three months (Elongation-rooting phase). The proliferation and development of shoots were affected by the basal medium, explant size and density. The optimal shoot proliferation (18.1) was observed when 4 buds clusters were cultured at the density of 2 clusters per jar in MS medium. Separated shoots of 4.5 to 6 cm length exhibited the optimal in vitro development in terms of leaf length and greening, and root number and length when cultured on MS medium. In addition, these shoots reached the highest acclimation frequency with 80%. Our results would be utilized for an efficient propagation of plantlets of cv. '16-bis' , a selected date palm cultivar resistant to the bayoud disease.

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Multiple bud cultures of ‘Barhee’ date palm (Phoenix dactylifera) and physiological status of regenerated plants

Cited by 38 publications

References 42 publications

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Micropropagation of Date Palm: A Review

Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

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