Multiple biological activities of a partially purified leukocytic endogenous mediator

Kampschmidt, R F; Upchurch, H F; Eddington, CL; La, Pulliam

doi:10.1152/ajplegacy.1973.224.3.530

Cited by 109 publications

(41 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A second possibility was that islets are "committed" to inhibition of insulin secretion soon after they are exposed to IL 1 but that > 5 h are required for the inhibitory effect to develop. The latter possibility was tested by exposing islets to IL 1 for short time periods (1,3, or 5 h) and then removing the IL 1 from the medium. After this 1-, 3-, or 5-h pulse with IL 1, the islets were maintained in culture as described in Methods.…”

Section: Resultsmentioning

confidence: 99%

“…IL 1 is recognized as an important mediator of inflammation and immunity and exerts effects on a number of cellular targets. Biological effects of IL 1 include activation of and induction ofproliferation by T lymphocytes (1), induction of fever by direct action on the hypothalamus (2), and stimula-tion of acute-phase protein synthesis by hepatocytes (3). A recently recognized effect of IL 1 is inhibition of insulin secretion by isolated pancreatic islets (4-1 1).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Interleukin 1 inhibits insulin secretion from isolated rat pancreatic islets by a process that requires gene transcription and mRNA translation.

Hughes¹,

Colca²,

Easom³

et al. 1990

J. Clin. Invest.

View full text Add to dashboard Cite

Recombinant human IL 1X1 inhibits glucose-induced insulin secretion from isolated pancreatic islets and from purified j3-cells obtained by fluorescence-activated cell sorting (FACS) of dispersed islet cells. Brief (1 h) exposure of isolated islets to IL I produces sustained inhibition of insulin secretion for at least 17 h after the IL 1 has been removed from the culture medium. An inhibitory effect of IL 1 on insulin secretion is not observed when islets are coincubated with an inhibitor of DNA transcription (actinomycin D). This finding indicates that the inhibitory effect of IL 1 on insulin secretion requires transcription of one or more genes during the first hour of exposure of islets to IL 1. The inhibitory effect of IL 1 on insulin secretion also requires mRNA translation, because three structurally distinct inhibitors of protein synthesis (cycloheximide, anisomycin, and puromycin) prevent IL 1-induced inhibition of insulin secretion when added to islets after the 1-h exposure to IL 1. Two-dimensional gel electrophoresis of islet proteins metabolically labeled with I35Slmethionine demonstrates that IL 1 augments the expression of a 65-kD (pl 6.5) protein by > 2.5-fold. These findings indicate that biochemical events occurring within 1 h of exposure of islets to IL 1 lead to an inhibition of insulin secretion that persists for at least 17 h after the removal of IL 1. One of the early biochemical effects of IL 1 on islets is gene transcription (0-1 h), which is followed by mRNA translation (after 1 h). Our results suggest that the inhibitory effect of IL 1 on insulin secretion is mediated by protein(s) whose synthesis is induced by IL 1. (J. Clin. Invest.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Interleukin 1 inhibits insulin secretion from isolated rat pancreatic islets by a process that requires gene transcription and mRNA translation.

Hughes¹,

Colca²,

Easom³

et al. 1990

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…These alterations in systemic metabolism are akin to those observed during inflammation and infection (1,9,14,17,16) and can be produced in healthy animals by the injection of leukocyte-derived factors (6,15 to provoke an increase in haptoglobin and seromucoid (15). The absence of a significant change in hematocrit and plasma glucose indicates that the observed changes in plasma constituents are not the result of changes in plasma volume and that poly(ICLC) is selective in its effect on host metabolism.…”

Section: Repeated Intramuscular Injections Of a Modified Poly(i)-polymentioning

confidence: 91%

“…Fever is presumably produced as a result of poly(I) -poly(C)-induced release of endogenous pyrogen from leukocytes (10). A decrease in plasma zinc can be elicited by other factors also derived from leukocytes (6). Under certain conditions, these leukocytederived factors can also stimulate the uptake of amino acids by the liver and produce increases in plasma acute-phase globulins in experimental animals (6,15 maintained in light-and temperature-controlled rooms (12 h of light and 12 h of dark, 25°C) for 1 week before use.…”

mentioning

confidence: 99%

“…A decrease in plasma zinc can be elicited by other factors also derived from leukocytes (6). Under certain conditions, these leukocytederived factors can also stimulate the uptake of amino acids by the liver and produce increases in plasma acute-phase globulins in experimental animals (6,15 maintained in light-and temperature-controlled rooms (12 h of light and 12 h of dark, 25°C) for 1 week before use. They were fed Purina pellets ad libitum until the initiation of the experiment, at which time food was withheld from all animals to obviate any differences that might arise from druginduced anorexia.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Systemic Metabolic Alterations Associated with Repeated Injections of a Modified Polyriboinosinic-Polyribocytidylic Acid Complex

Powanda

Sammons

Stephen

1977

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Polyriboinosinic acid-polyribocytidylic acid complexed with poly-l-lysine and injected intramuscularly into rats (0.3 or 3.0 mg/kg) produced fever, altered leukocyte count, slightly depressed plasma zinc, increased amino acid uptake into liver, and increased plasma acute-phase globulins two-to threefold. It is suggested that these systemic metabolic alterations are indicative of a mild inflammatory response to this drug. The metabolic alterations may have to be taken into consideration when polyriboinosinic acid-polyribocytidylic acid complexed with poly-1-lysine is used in therapy.

show abstract

Studies on the release of cell‐associated interleukin 1 by paraformaldehyde‐treated murine macrophages

et al. 1988

View full text Add to dashboard Cite

Experiments are presented demonstrating the release of interleukin 1 (IL1) by lipopolysaccharide-stimulated murine macrophages (M phi) after treatment of the cells with paraformaldehyde (PFA). The IL1 activity set free was completely blocked by a rabbit anti-mouse IL1 alpha antiserum. Immunoprecipitation studies revealed that the IL1 released was of Mr 33 kDa, 23 kDa and 17 kDa. Incubation of PFA-treated M phi in the presence of leupeptin reduced the amount of detectable low molecular mass (i.e. 23 kDa and 17 kDa) IL1 alpha. Addition of leupeptin to PFA-treated M phi also reduced the amount of released IL1 activity, suggesting that proteolytic modification of the IL1 alpha precursor is involved in the generation of biological active IL1 alpha. It is proposed that the cell-associated 33-kDa IL1 is released from the M phi by vesiculation of parts of the M phi cell membrane, followed by a proteolytic cleavage step.

show abstract

Multiple biological activities of a partially purified leukocytic endogenous mediator

Cited by 109 publications

References 0 publications

Interleukin 1 inhibits insulin secretion from isolated rat pancreatic islets by a process that requires gene transcription and mRNA translation.

Interleukin 1 inhibits insulin secretion from isolated rat pancreatic islets by a process that requires gene transcription and mRNA translation.

Systemic Metabolic Alterations Associated with Repeated Injections of a Modified Polyriboinosinic-Polyribocytidylic Acid Complex

Studies on the release of cell‐associated interleukin 1 by paraformaldehyde‐treated murine macrophages

Contact Info

Product

Resources

About