Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery

Sw, Lee; Musa, Najiah; Wee, Wendy; Zahrol, A; Musa, Nadirah

doi:10.1016/s1671-2927(08)60273-4

Cited by 22 publications

(14 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inoculated media underwent incubation for 24-48 h at room temperature (25-28°C). All the suspected bacteria colony were identified using conventional biochemical tests [ 4 ] and followed with commercial identification kit (BBL, USA) [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

“…[ 3 ], A. hydrophila caused significant economic loss in channel catfish, Ictalurus punctatus farming in the United States. There were also reports on A. hydrophila infection in other aquatic animals involving giant freshwater prawn, Macrobrachium rosenbergii [ 4 ] and American bullfrog, and Rana catesbeiana [ 5 ]. Edwardsiellosis frequently resulted in high mortality to the farmed fish when the fish were under stress due to poor husbandry and bad weather conditions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibiotic and heavy metal resistance of Aeromonas hydrophila and Edwardsiella tarda isolated from red hybrid tilapia (Oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis

Wei¹,

Wee²

2017

Vet World

View full text Add to dashboard Cite

Aim:The aim of this study is to identify antibiogram and heavy metal resistance pattern of Aeromonas hydrophila and Edwardsiella tarda isolated from red hybrid tilapia (Oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis in four commercial fish farms.Materials and Methods:A. hydrophila and E. tarda were isolated using glutamate starch phenol red and xylose lysine deoxycholate (Merck, Germany) as a selective medium, respectively. All the suspected bacterial colonies were identified using conventional biochemical tests and commercial identification kit (BBL Crystal, USA). Susceptibility testing of present bacterial isolates to 16 types of antibiotics (nalidixic acid, oxolinic acid, compound sulfonamides, doxycycline, tetracycline, novobiocin, chloramphenicol, kanamycin, sulfamethoxazole, flumequine, erythromycin, ampicillin, spiramycin, oxytetracycline, amoxicillin, and fosfomycin) and four types of heavy metals (mercury, chromium, copper, and zinc) were carried out using disk diffusion and two-fold agar dilution method, respectively.Results:Three hundred isolates of A. hydrophila and E. tarda were successfully identified by biochemical tests. Antibiotic susceptibility testing results showed that 42.2% of the bacterial isolates were sensitive to compound sulfonamides, sulfamethoxazole, flumequine, oxytetracycline, doxycycline, and oxolinic acid. On the other hand, 41.6% of these isolates were resistant to novobiocin, ampicillin, spiramycin, and chloramphenicol, which resulted for multiple antibiotic resistance index values 0.416. Among tested heavy metals, bacterial isolates exhibited resistant pattern of Zn2+ > Cr6+ > Cu2+ > Hg2+.Conclusion:Results from this study indicated that A. hydrophila and E. tarda isolated from coinfected farmed red hybrid tilapia were multi-resistant to antibiotics and heavy metals. These resistant profiles could be useful information to fish farmers to avoid unnecessary use of antimicrobial products in the health management of farmed red hybrid tilapia.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibiotic and heavy metal resistance of Aeromonas hydrophila and Edwardsiella tarda isolated from red hybrid tilapia (Oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis

Wei¹,

Wee²

2017

Vet World

View full text Add to dashboard Cite

show abstract

“…() and Lee et al . (). The tissue samples were homogenized in 90‐mL sterile physiological saline (0.85% NaCl, w/v) using a masticator (IUL Instruments, Barcelona, Spain) for 50 s. Subsequently, 10‐fold serial dilutions (up to 10 −5 ) of the homogenate were prepared using sterile physiological saline.…”

Section: Methodsmentioning

confidence: 97%

Effect by Gamma Irradiation and Low-Temperature Storage on Bacteriological Profile of Edible Estuarine CrabScylla serrata

Arshad¹,

Kappalli²,

Hatha

et al. 2015

Journal of Food Processing and Preservation

View full text Add to dashboard Cite

The effect of dose-dependent gamma irradiation and subsequent cold storage on bacteriological profile of tissues derived from Scylla serrata was evaluated. Irradiation at 0.5, 1.0 and 2.0 kGy significantly reduced the (P = 0.0001) total plate count (TPC) by 1.0, 2.0 and 2.5 log units, respectively. Treatments of 1.0 and 2.0 kGy irradiation reduced total coliform (TC) count below 1 most probable number (MPN)/g in the muscle and hepatopancreas and below 50 MPN/g in the gills of S. serrata. Irradiated tissues stored at −20C showed significant reduction in TPC and TC. Bacillus spp. was predominant in the tissues before and after gamma irradiation. While live crab tissues harbored both gram-positive and gram-negative bacteria in equal proportion, more than 90% of them from irradiated tissues were gram-positive. Biochemical and 16S rRNA gene-based study on generic composition of heterotrophic bacteria revealed fairly good diversity in nonirradiated tissues and the dose-dependent gamma irradiation significantly reduced their diversity concomitantly. PRACTICAL APPLICATIONSThe mud crab Scylla serrata in which the present study was conducted has immense market demand all over the world. The present study clearly demonstrated the possibility to utilize gamma irradiation to reduce the microbial contamination and thereby extend the shelf life of this expensive shellfish under refrigerated temperatures.

show abstract

“…There are various resistance factors which V. parahaemolyticus carry such as: cobalt, zinc, cadmium, and chromium resistance genes [ 78 ]. This can also explain its possible survival rate with M. rosenbergii , which could have been exposed to toxic substances during its life cycle [ 79 , 80 ]. During the course of evolution, the bacteria must have acquired these resistance genes on prolonged exposure while surviving together with the host, which is M. rosenbergii .…”

Section: Growth Conditions Of the Host And Pathogenmentioning

confidence: 99%

Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium

Tiruvayipati

Bhassu

2016

Gut Pathog

View full text Add to dashboard Cite

Macrobrachium rosenbergii is well-known as the giant freshwater prawn, and is a commercially significant source of seafood. Its production can be affected by various bacterial contaminations. Among which, the genus Vibrio shows a higher prevalence in aquatic organisms, especially M. rosenbergii, causing food-borne illnesses. Vibrio parahaemolyticus, a species of Vibrio is reported as the main causative of the early mortality syndrome. Vibrio parahaemolyticus infection in M. rosenbergii was studied previously in relation to the prawn’s differentially expressed immune genes. In the current review, we will discuss the growth conditions for both V. parahaemolyticus and M. rosenbergii and highlight the role of magnesium in common, which need to be fully understood. Till date, there has not been much research on this aspect of magnesium. We postulate a model that screens a magnesium-dependent pathway which probably might take effect in connection with N-acetylglucosamine binding protein and chitin from V. parahaemolyticus and M. rosenbergii, respectively. Further studies on magnesium as an environment for V. parahaemolyticus and M. rosenbergii interaction studies will provide seafood industry with completely new strategies to employ and to avoid seafood related contaminations.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-016-0097-1) contains supplementary material, which is available to authorized users.

show abstract

Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery

Cited by 22 publications

References 16 publications

Antibiotic and heavy metal resistance of Aeromonas hydrophila and Edwardsiella tarda isolated from red hybrid tilapia (Oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis

Antibiotic and heavy metal resistance of Aeromonas hydrophila and Edwardsiella tarda isolated from red hybrid tilapia (Oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis

Effect by Gamma Irradiation and Low-Temperature Storage on Bacteriological Profile of Edible Estuarine CrabScylla serrata

Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium

Contact Info

Product

Resources

About