Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium

Tiruvayipati, Suma; Bhassu, Subha

doi:10.1186/s13099-016-0097-1

Cited by 24 publications

(11 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GlcNAc-binding protein A (GbpA) is a vital protein released by Vibrio bacteria for binding with chitin subunits, GlcNAc [25]. GbpA’s interaction with chitin and associated components was also investigated in M. rosenbergii previously [26, 27].…”

Section: Discussionmentioning

confidence: 99%

Differential transcriptome analysis of the disease tolerant Madagascar–Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background Penaeus monodon is the second most widely cultured marine shrimp species in the global shrimp aquaculture industry. However, the growth of P. monodon production has been constantly impaired by disease outbreaks. Recently, there is a lethal bacterial infection, known as acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus AHPND strain ( Vp AHPND ), which led to mass mortalities in P. monodon . Unfortunately, there is still insufficient knowledge about the underlying immune response of P. monodon upon AHPND infection. The present study aims to provide an insight into the antibacterial immune response elicited by P. monodon hepatopancreas towards AHPND infection. Methods We have employed high-throughput RNA-Seq technology to uncover the transcriptome changes of P. monodon hepatopancreas when challenged with Vp AHPND . The shrimps were challenged with Vp AHPND through immersion method with dissected hepatopancreas samples for the control group (APm-CTL) and treatment group at 3 (APm-T3), 6 (APm-T6), and 24 (APm-T24) hours post-AHPND infection sent for RNA-Seq. The transcriptome de novo assembly and Unigene expression determination were conducted using Trinity, Tgicl, Bowtie2, and RSEM software. The differentially expressed transcripts were functionally annotated mainly through COG, GO, and KEGG databases. Results The sequencing reads generated were filtered to obtain 312.77 Mb clean reads and assembled into 48662 Unigenes. Based on the DEGs pattern identified, it is inferred that the PAMPs carried by Vp AHPND or associated toxins are capable of activating PRRs, which leads to subsequent pathway activation, transcriptional modification, and antibacterial responses (Phagocytosis, AMPs, proPO system). DAMPs are released in response to cell stress or damage to further activate the sequential immune responses. The comprehensive interactions between Vp AHPND , chitin, GbpA, mucin, chitinase, and chitin deacetylase were postulated to be involved in bacterial colonization or antibacterial response. Conclusions The outcomes of this research correlate the different stages of P. monodon immune response to different time points of AHPND infection. This finding supports the development of biomarkers for the detection of early stages of Vp AHPND colonization in P. monodon through host immune expression changes. The potential genes to be utilized as biomarkers include but not limited to C-type lectin, HMGB1, IMD, AL...

show abstract

Section: Discussionmentioning

confidence: 99%

Differential transcriptome analysis of the disease tolerant Madagascar–Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The genus Vibrio is ubiquitously distributed in marine and freshwater environments (Tiruvayipati & Bhassu, ) and is prevalent high in aquatic organisms (Ceccarelli & Colwell, ). Vibriosis has been particularly devastating disease in the marine environment, affecting many species of fish (Wang & Leung, ).…”

Section: Introductionmentioning

confidence: 99%

Histopathological changes in giant freshwater prawnMacrobrachium rosenbergii(de Man 1879) fed with probioticBacillus licheniformisupon challenge withVibrio alginolyticus

et al. 2017

View full text Add to dashboard Cite

The effect of dietary supplementation of probiotic bacterium Bacillus licheniformis on the histopathological changes in Macrobrachium rosenbergii juveniles (4.0 AE 0.02 g) challenged with known pathogenic strain of Vibrio alginolyticus are reported. Two isocaloric basal diets supplemented with probiotic bacteria B. licheniformis (1.0 9 10 9 cfu/g feed) and other without probiotic supplementation were fed to the M. rosenbergii juveniles for 45 days. The histological observations revealed no significant changes in the hepatopancreas and gut tissues of both the experimental and the control groups which indicate that the present bacterium is a safe candidate probiont for the host. Prawns were challenged with V. alginolyticus after 45 days of feeding with probiotic diet. The histopathological studies of the hepatopancreas revealed that M. rosenbergii fed with probiotic-supplemented diet showed less changes as compared to the prawns fed with control diet on second and fourth day of post-experimental challenge with V. alginolyticus. Histopathological observations revealed that the gills of the prawns fed with control diet were severely affected in comparison to the prawns fed with probiotic-supplemented diet after challenging with V. alginolyticus. Results from this study revealed the improved protection by dietary incorporation of B. licheniformis in reducing the histopathological manifestations due to V. alginolyticus infection in freshwater prawn. K E Y W O R D S Bacillus licheniformis, histopathology, Macrobrachium rosenbergii, probiotic

show abstract

“…Due to higher gbpA expression at this stage the chances of the binding ability of gbpA to chitin could be higher. This hypothesis generated from our study can only be proved with further research targeting the moulting phase of prawn [ 29 ].…”

Section: Discussionmentioning

confidence: 96%

Host, pathogen and environment: a bacterial gbpA gene expression study in response to magnesium environment and presence of prawn carapace and commercial chitin

Tiruvayipati

Bhassu

2016

Gut Pathog

Self Cite

View full text Add to dashboard Cite

BackgroundVibrio parahaemolyticus is a Gram-negative halophilic bacterium which is found largely in estuarine and coastal waters. The bacteria has been a main focus in gastro-intestinal infections caused primarily due to the consumption of contaminated seafood. It was shown to survive in magnesium concentrations as high as 300 mM which are toxic to various other micro-organisms. Several genes of V. parahaemolyticus were studied, among which gbpA (N-acetyl glucosamine binding protein) was reported in Vibrio cholerae.MethodsThe current study investigates the V. parahaemolyticus gbpA gene expression at different concentrations of magnesium sulfate heptahydrate (MgSO4·7H2O, chosen as the magnesium environment), in the presence of the host’s (prawn) carapace and the mimicked carapace [commercial chitin flakes (Sigma)]. The concentrations of MgSO4·7H2O utilized were approximately 0, 1, 75, 137, 225 and 300 mM. These were selected based upon the survival conditions required by prawn and bacteria, respectively. 0.05 gm/3 ml of carapace (by dry weight) and commercial chitin flakes were used in the experiments. Bacterial count was performed for the biological triplicates for the 3 experimental setups. The genome of Vibrio parahaemolyticus PCV08-7 (VPPCV08-7) was used as a reference, based on whose translated gbpA gene the probable protein-chemical interactions were determined on the STITCH database.ResultsThe GbpA protein was shown to interact with chitin on the STITCH database. In our experiments, the gbpA showed lower gene expression levels at different MgSO4·7H2O concentrations in the presence of chitin and carapace, than with the presence of only MgSO4•7H2O. In addition, the bacterial count for various concentrations of magnesium used, revealed a distinct decrease in bacterial count within and among each of the three experimental setups.ConclusionIn the presence of only magnesium, an increase in the gbpA expression with neither chitin nor carapace and vice versa supported by the results from the bacterial counts could help further studies to prove that the moulting phase of prawns may trigger increased expression of the V. parahaemolyticus gbpA gene.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-016-0105-5) contains supplementary material, which is available to authorized users.

show abstract

Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium

Cited by 24 publications

References 70 publications

Differential transcriptome analysis of the disease tolerant Madagascar–Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction

Differential transcriptome analysis of the disease tolerant Madagascar–Malaysia crossbred black tiger shrimp, Penaeus monodon hepatopancreas in response to acute hepatopancreatic necrosis disease (AHPND) infection: inference on immune gene response and interaction

Histopathological changes in giant freshwater prawnMacrobrachium rosenbergii(de Man 1879) fed with probioticBacillus licheniformisupon challenge withVibrio alginolyticus

Host, pathogen and environment: a bacterial gbpA gene expression study in response to magnesium environment and presence of prawn carapace and commercial chitin

Contact Info

Product

Resources

About