Multiple antibiotic resistance and DNA methylation in Enterobacteriaceae isolates from different environments

Yuan, Wei; Zhang, Yongli; Riaz, Luqman; Yang, Qi; Du, Bingbing; Wang, Ruifei

doi:10.1016/j.jhazmat.2020.123822

Cited by 28 publications

(12 citation statements)

References 65 publications

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“…In previous work, less than 1.2 log of intI1 was removed by hospital wastewater treatment, and intI1 remained detectable at 8×10 −5 copies/16S rRNA gene (Timraz et al 2017). Forty-three ARG cassettes mediated by intI1 were detected with great diversity in hospital wastewater (Yuan et al 2021). As indicated in Table A.10, the presence of the last-line antibiotic (carbapenem) resistance gene bla GES-1 was strongly associated with the presence of bla OXA-10 .…”

Section: Occurrence Of Args In Hospital Wastewatermentioning

confidence: 71%

Occurrence and removal of antibiotics, antibiotic resistance genes, and bacterial communities in hospital wastewater

Yao

Yang

et al. 2021

Environ Sci Pollut Res

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Hospital wastewater contains a variety of human antibiotics and pathogens, which makes the treatment of hospital wastewater essential. However, there is a lack of research on these pollutants at hospital wastewater treatment plants. In this study, the characteristics and removal of antibiotics and antibiotic resistance genes (ARGs) in the independent treatment processes of hospitals of different scales (primary hospital, H1; secondary hospital, H2; and tertiary hospital, H3) were investigated. The occurrence of antibiotics and ARGs in wastewater from three hospitals varied greatly. The first-generation cephalosporin cefradine was detected at a concentration of 2.38 μg/L in untreated wastewater from H1, while the fourth-generation cephalosporin cefepime had the highest concentration, 540.39 μg/L, at H3. Ofloxacin was detected at a frequency of 100% and had removal efficiencies of 44.2%, 51.5%, and 81.6% at H1, H2, and H3, respectively. The highest relative abundances of the βlactam resistance gene bla GES-1 (1.77×10 −3 copies/16S rRNA), the quinolone resistance gene qnrA (8.81×10 −6 copies/16S rRNA), and the integron intI1 (1.86×10 −4 copies/16S rRNA) were detected in the treated wastewater. The concentrations of several ARGs were increased in the treated wastewater (e.g. bla OXA-1 , bla OXA-10 , and bla TEM-1 ). Several pathogenic or opportunistic bacteria (e.g. Acinetobacter, Klebsiella, Aeromonas, and Pseudomonas) were observed at high relative abundances in the treated wastewater. These results suggested the co-occurrence of antibiotics, ARGs, and antibiotic-resistant pathogens in hospital wastewater, and these factors may spread into the receiving aquatic environment.

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Section: Occurrence Of Args In Hospital Wastewatermentioning

confidence: 71%

Occurrence and removal of antibiotics, antibiotic resistance genes, and bacterial communities in hospital wastewater

Yao

Yang

et al. 2021

Environ Sci Pollut Res

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show abstract

“…In previous work, less than 1.2 log of intI1 was removed by hospital wastewater treatment, and intI1 remained detectable at 8×10 − 5 copies/16S rRNA gene (Timraz et al 2017). Forty-three ARG cassettes mediated by intI1 were detected with great diversity in hospital wastewater (Yuan et al 2021). In the present study, the relative abundances of ARGs were between 1.01×10 − 9 and 1.77×10 − 3 copies/16S rRNA gene in the treated wastewaters.…”

Section: Removal Of Args In Different Hwwtpsmentioning

confidence: 82%

Occurrence and Removal of Antibiotics, Antibiotic-Resistance Genes and Bacterial Communities in Hospital Wastewater

Yao

Yang

et al. 2021

Preprint

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Hospital wastewater contains a variety of human-related antibiotics and pathogens, which makes the treatment of hospital wastewater essential. However, there is a lack of research on these pollutants in hospital wastewater treatment plants. In this study, the characteristics and removal of antibiotics and antibiotic resistance genes (ARGs) in independent treatment processes of hospitals of different scales (primary hospital H1, secondary hospital H2, and tertiary hospital H3) were investigated. The occurrence of antibiotics and ARGs in wastewater from three hospitals varied greatly. The first-generation cephalosporin cefradine was detected at a concentration of 2.38 μg/L in untreated wastewater of H1, while the fourth-generation cephalosporin cefepime had the highest concentration, 540.39 μg/L, in H3. Ofloxacin was detected at a frequency of 100% and had removal efficiencies of 44.2%, 51.5%, and 81.6% for H1, H2, and H3, respectively. The highest relative abundances of the β-lactam resistance gene blaGES-1 (1.77×10-3 copies/16S rRNA), quinolone resistance gene qnrA (8.81×10-6 copies/16S rRNA), and integron intI1 (1.86×10-4 copies/16S rRNA) were detected in the treated wastewaters. The concentrations of several ARGs were increased in the treated wastewater (e.g., blaOXA-1, blaOXA-10 and blaTEM-1). Several pathogenic or opportunistic bacteria (e.g., Acinetobacter, Klebsiella, Aeromonas, and Pseudomonas) were observed with high relative abundances in the treated wastewater. These results suggested the co-occurrence of novel antibiotics, ARGs, and antibiotic-resistant pathogens in hospital wastewater that may spread into the receiving water environment.

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“…Therefore, DNA methyltransferases that recognize CCAGG and CCTGG sequences may be modifying factors in EM resistance, if not the main factor. The relationship between bacterial responses to antibiotics and DNA methylation status (33) is an important subject for future analysis by 16S rDNA meta-epigenetics.…”

Section: Discussionmentioning

confidence: 99%

Extension of bacterial rDNA sequencing to concurrent epigenetic analysis and its application to 16S meta-epigenetics

Nishimura

Tanaka

Murata

et al. 2022

Preprint

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Although polymerase chain reaction (PCR) amplification and sequencing of the 16S rDNA region has been used in a wide range of scientific fields, it does not provide DNA methylation information. We describe a simple add-on method to investigate 5-methylcytosine residues in the bacterial 16S rDNA region from clinical samples or flora. Single-stranded bacterial DNA after bisulfite conversion was preferentially amplified with multiple displacement amplification (MDA) at pH neutral, and the 16S rDNA region was analyzed using nested bisulfite PCR and sequencing. 16S rDNA bisulfite sequencing can provide clinically important bacterial DNA methylation status concurrently with intact 16S rDNA sequence information. We used this approach to identify novel methylation sites and a methyltransferase (M. MmnI) in Morganella morganii. Next, we analyzed bacterial flora from clinical specimens of small amount and identified different methylation motifs among Enterococcus faecalis strains. The method developed here, referred to as "add-on" to the conventional 16S rDNA analysis, is the most clinically used bacterial identification genetic test, which provides additional information that could not be obtained with the conventional method. Since the relationship between drug resistance in bacteria and DNA methylation status has been reported, bacterial epigenetic information would be useful in clinical testing as well. Our analysis suggests that M. MmnI has a promotive effect on erythromycin resistance. 16S rDNA bisulfite PCR and sequencing coupled with MDA at pH neutral is a useful add-on tool for analyzing 16S meta-epigenetics.

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Multiple antibiotic resistance and DNA methylation in Enterobacteriaceae isolates from different environments

Cited by 28 publications

References 65 publications

Occurrence and removal of antibiotics, antibiotic resistance genes, and bacterial communities in hospital wastewater

Occurrence and removal of antibiotics, antibiotic resistance genes, and bacterial communities in hospital wastewater

Occurrence and Removal of Antibiotics, Antibiotic-Resistance Genes and Bacterial Communities in Hospital Wastewater

Extension of bacterial rDNA sequencing to concurrent epigenetic analysis and its application to 16S meta-epigenetics

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