Multiphoton tomography in differentiation of morphological and molecular subtypes of breast cancer: A quantitative analysis

Gubarkova, Ekaterina V.; Elagin, Vadim; Dudenkova, Varvara V.; Kuznetsov, Sergey S.; Karabut, Maria; Potapov, Arseniy L.; Vorontsov, Dmitry А.; Vorontsov, А. Yu.; Sirotkina, Marina A.; Zagaynova, Elena V.; Gladkova, Natalia D.

doi:10.1002/jbio.202000471

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…ZnO NPs could be used to in situ upconvert NIR to visible light through the second harmonic generation (SHG) mechanism. The second-order nonlinearity of ZnO NPs was several orders of magnitude higher than that in SHG-producing biological media, such as fibrillar collagens, which correspondingly indicated that the efficiency of nonlinear upconversion by ZnO NPs would also be significantly higher. ,, …”

Section: Resultsmentioning

confidence: 98%

Antitumor Activity of the Zinc Oxide Nanoparticles Coated with Low-Molecular-Weight Heparin and Doxorubicin Complex In Vitro and In Vivo

Zhang

Liu

et al. 2022

Mol. Pharmaceutics

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Various metal oxide nanomaterials have been widely used as carriers to prepare pH-sensitive nanomedicines to respond to the acidic tumor microenvironment promoting antitumor efficiency. Herein, we used zinc oxide nanoparticles (ZnO NPs) as metal oxide nanomaterial coated with low-molecular-weight heparin (LMHP) and doxorubicin (DOX) complex (LMHP-DOX) to prepare ZnO-LD NPs for controllable pH-triggered DOX release on the targeted site. Our results indicated that the released DOX from ZnO-LD NPs was pH-sensitive. The oxygen produced by ZnO-LD NPs in H 2 O 2 solution was observed in in vitro experiment. The ZnO-LD NPs entered into both PC-3M and 4T1 tumor cells via clathrin-mediated endocytosis and micropinocytosis pathway. The intracellular reactive oxygen species (ROS) generated by ZnO-LD NPs could significantly increase the caspase 3/7 level, leading to tumor cell apoptosis. The in vitro and in vivo antitumor activity was confirmed in PC-3M and 4T1 cell lines or tumor-bearing mice models. The in vivo and in vitro tumor images via second-order nonlinearity of ZnO-LD NPs indicated that ZnO-LD NPs could penetrate deep into the tumor tissues. Therefore, the ZnO-LD NPs developed in our study could provide an efficient approach for the preparation of pH-sensitive nano delivery systems suitable for tumor therapy and imaging.

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Section: Resultsmentioning

confidence: 98%

Antitumor Activity of the Zinc Oxide Nanoparticles Coated with Low-Molecular-Weight Heparin and Doxorubicin Complex In Vitro and In Vivo

Zhang

Liu

et al. 2022

Mol. Pharmaceutics

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“…The SHG is excited by non-centrosymmetric molecules, such as collagen I in dermis [111][112][113][114] and crystallized urea structures in the stratum corneum of glabrous skin [115] can be used for their visualization in the skin. Collagen I is synthesized by fibroblasts in the dermis and, therefore, SHG microscopy can be a suitable method for fibroblast imaging.…”

Section: Healthy Skin Fibroblasts and Ecm Studied By Tpe-fmmentioning

confidence: 99%

Review of optical methods for noninvasive imaging of skin fibroblasts—From in vitro to ex vivo and in vivo visualization

Nikolaev,

Kistenev,

Kröger

et al. 2024

Journal of Biophotonics

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Fibroblasts are among the most common cell types in the stroma responsible for creating and maintaining the structural organization of the extracellular matrix (ECM) in the dermis, skin regeneration, and a range of immune responses. Until now, the processes of fibroblast adaptation and functioning in a varying environment have not been fully understood. Modern laser microscopes are capable of studying fibroblasts in vitro and ex vivo. One‐photon‐ and two‐photon‐excited fluorescence microscopy, Raman spectroscopy/micro‐spectroscopy are well‐suited non‐invasive optical methods for fibroblast imaging in vitro and ex vivo. In vivo staining‐free fibroblast imaging is not still implemented. The exception is fibroblast imaging in tattooed skin. Although in vivo non‐invasive staining‐free imaging of fibroblasts in the skin has not yet been implemented, it is expected in the future. This review summarizes the state of the art in fibroblast visualization using optical methods and discusses the advantages, limitations, and prospects for future non‐invasive imaging.This article is protected by copyright. All rights reserved.

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“…They found five ‘collagen signatures’ associated with the invasion front of breast cancer cells and, by using NLOM images, were able to train a model to provide a signature score, giving a personalized approach to the prognosis and treatment of breast cancers. Similarly, Gubarkova et al used NLOM to characterize elastin fiber morphology in different forms of breast cancer, demonstrating that NLOM can be used in the morphological characterization of different states of the same disease [ 76 ].…”

Section: Imaging the Ecm Components: Past Present And Future Challengesmentioning

confidence: 99%

Optical Microscopy and the Extracellular Matrix Structure: A Review

Poole

Mostaço-Guidolin

2021

Cells

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Biological tissues are not uniquely composed of cells. A substantial part of their volume is extracellular space, which is primarily filled by an intricate network of macromolecules constituting the extracellular matrix (ECM). The ECM serves as the scaffolding for tissues and organs throughout the body, playing an essential role in their structural and functional integrity. Understanding the intimate interaction between the cells and their structural microenvironment is central to our understanding of the factors driving the formation of normal versus remodelled tissue, including the processes involved in chronic fibrotic diseases. The visualization of the ECM is a key factor to track such changes successfully. This review is focused on presenting several optical imaging microscopy modalities used to characterize different ECM components. In this review, we describe and provide examples of applications of a vast gamut of microscopy techniques, such as widefield fluorescence, total internal reflection fluorescence, laser scanning confocal microscopy, multipoint/slit confocal microscopy, two-photon excited fluorescence (TPEF), second and third harmonic generation (SHG, THG), coherent anti-Stokes Raman scattering (CARS), fluorescence lifetime imaging microscopy (FLIM), structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED), ground-state depletion microscopy (GSD), and photoactivated localization microscopy (PALM/fPALM), as well as their main advantages, limitations.

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Multiphoton tomography in differentiation of morphological and molecular subtypes of breast cancer: A quantitative analysis

Cited by 6 publications

References 42 publications

Antitumor Activity of the Zinc Oxide Nanoparticles Coated with Low-Molecular-Weight Heparin and Doxorubicin Complex In Vitro and In Vivo

Antitumor Activity of the Zinc Oxide Nanoparticles Coated with Low-Molecular-Weight Heparin and Doxorubicin Complex In Vitro and In Vivo

Review of optical methods for noninvasive imaging of skin fibroblasts—From in vitro to ex vivo and in vivo visualization

Optical Microscopy and the Extracellular Matrix Structure: A Review

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