Multiphoton imaging with blue-diode-pumped SESAM-modelocked Ti:sapphire oscillator generating 5 nJ 82 fs pulses

Rohrbacher, Andreas; Olarte, Omar E.; Villamaina, Vesna; Loza-Álvarez, Pablo; Resan, Bojan

doi:10.1364/oe.25.010677

Cited by 29 publications

(20 citation statements)

References 28 publications

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“…Moreover, all current works have done based on such a model. Since 2009, the performance of LD-pumped Ti:sapphire lasers has been gradually improved, approaching the performance of traditional Ti:sapphire lasers in terms of high power, [59][60][61][62] wide wavelength tunability, 61,63 and short pulse duration. [64][65][66][67][68] 3.1 | Blue-laser-diode pumped Ti:sapphire oscillators…”

Section: Laser-diode Pumped Ti: Sapphire Lasersmentioning

confidence: 99%

“…In 2017, Rohrbacher et al employed two 2.9 W 450 nm blue LDs to bilaterally pump a 4 mm long Ti: sapphire crystal, and used a SESAM to start and maintain the mode-locking operation. 60 The mode-locked oscillator could obtain a maximum output power of 460 mW with 82 fs pulse width. The optical-to-optical conversion efficiency of blue LD directly pumped Ti:sapphire mode-locked laser is close to 8%.…”

Section: Laser-diode Pumped Ti: Sapphire Lasersmentioning

confidence: 99%

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Review of laser‐diode pumped Ti:sapphire laser

Liu

Sun

Zheng

et al. 2021

Micro & Optical Tech Letters

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Ti:sapphire laser has an important position in ultrashort pulse generation and wavelength tuning. The use of expensive frequency-doubled diode-pumped solid-state laser as the pump source limits the promotion of its application to a certain extent. With the maturity of high-power bluegreen laser diodes (LDs), low-cost LD directly pumped Ti: sapphire laser becomes possible. After more than 10 years of development, the output parameters of LD pumped Ti: sapphire lasers have been greatly improved. Moreover, the lasers have been applied to optical frequency combs, multiphoton microscopy imaging, and so forth, both of which have achieved impressive results.

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Section: Laser-diode Pumped Ti: Sapphire Lasersmentioning

confidence: 99%

Section: Laser-diode Pumped Ti: Sapphire Lasersmentioning

confidence: 99%

Review of laser‐diode pumped Ti:sapphire laser

Liu

Sun

Zheng

et al. 2021

Micro & Optical Tech Letters

View full text Add to dashboard Cite

show abstract

“…In this case, three different excitation spectra can be generated, centered at three different wavelengths [164]. Mahou et al [68] have demonstrated the simultaneous acquisition of three colors using two spatially and temporally synchronized pulse trains.…”

Section: 1a Simultaneous Multicolor Detectionmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

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“…In addition, using near infrared (NIR) excitation wavelengths also increases the penetration depth, which is particularly significant for biomedical imaging [10][11][12]. Multiphoton microscopy (MPM) and coherent Raman scattering (CRS) microscopy are the most frequently applied nonlinear imaging technologies for biomedical microscopy [13][14][15][16][17][18]. The former mainly includes two-photon fluorescence (TPF), three-photon fluorescence (ThPF), second-harmonic generation (SHG), and third-harmonic generation (THG) microscopy.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Multidepth Multiphoton Microscopy Using Pixel-to-Pixel Focus-Switching

2020

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Multiphoton microscopy is a well-established technique for biomedical applications, but real-time multidepth multimodal multiphoton microscopy using non-imaging detection has barely been discussed. We demonstrate a novel label-free imaging system capable of generating multimodal multiphoton signals at different focal planes simultaneously. Two spatially overlapped and temporally interlaced beams are obtained by applying cost-effective electro-optic modulator (EOM)-based fast-switching light paths. The switching beams have different divergence properties, enabling imaging at different depths into samples. The EOM is synchronized to the pixel clock from the microscope, achieving pixel-to-pixel focus-switching. The capability of the imaging system is demonstrated by performing real-time multidepth two-photon fluorescence (TPF) and second-harmonic generation (SHG) imaging of freshly excised mouse lung lobes. TPF and SHG images are acquired at two wavelength ranges. One is between 415 and 455 nm, and the other is between 495 and 635 nm. The microenvironment of pulmonary alveoli is depicted by the distributions of both elastin fibers visualized by TPF and collagen fibers illustrated by SHG. Macrophages residing inside apparent alveolar lumens are also identified by TPF, which shows that the imaging system is capable of localizing biological objects in three dimensions and has the potential of monitoring in vivo cellular dynamics in the axial direction.

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Multiphoton imaging with blue-diode-pumped SESAM-modelocked Ti:sapphire oscillator generating 5 nJ 82 fs pulses

Cited by 29 publications

References 28 publications

Review of laser‐diode pumped Ti:sapphire laser

Review of laser‐diode pumped Ti:sapphire laser

Light-sheet microscopy: a tutorial

Real-Time Multidepth Multiphoton Microscopy Using Pixel-to-Pixel Focus-Switching

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