Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Yamada, Masao; Seki, Minoru

doi:10.1252/jcej.17we328

Cited by 20 publications

(11 citation statements)

References 112 publications

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“…UV irradiation is required for photosensitive hydrogels to induce light crosslinking. Alginate can be prepared by injecting ionic solutions [ 92 ]. Chen et al prepared an alginate-based microfluidic system, generated breast tumor cell spheroids, and tested them with doxorubicin.…”

Section: Methods For Mcss Generationmentioning

confidence: 99%

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

Shen

Cai

et al. 2021

Micromachines

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Three-dimensional multicellular spheroids (MCSs) have received extensive attention in the field of biomedicine due to their ability to simulate the structure and function of tissues in vivo more accurately than traditional in vitro two-dimensional models and to simulate cell–cell and cell extracellular matrix (ECM) interactions. It has become an important in vitro three-dimensional model for tumor research, high-throughput drug screening, tissue engineering, and basic biology research. In the review, we first summarize methods for MCSs generation and their respective advantages and disadvantages and highlight the advances of hydrogel and microfluidic systems in the generation of spheroids. Then, we look at the application of MCSs in cancer research and other aspects. Finally, we discuss the development direction and prospects of MCSs

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Section: Methods For Mcss Generationmentioning

confidence: 99%

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

Shen

Cai

et al. 2021

Micromachines

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“…The typical example of ionic crosslinking is the electrostatic interaction between negatively charged alginate chains and positively charged Ca 2+ ions. Ionic crosslinking in microfluidic channels can be achieved using five different strategies [ 26 ]: internal gelation, external gelation, rapid in-droplet mixing (chaotic advection), droplet merging (coalescence), and competitive ligand exchange.…”

Section: Microfluidic Production Of Spherical Matrix-type Microgelsmentioning

confidence: 99%

Crosslinking Strategies for the Microfluidic Production of Microgels

2021

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This article provides a systematic review of the crosslinking strategies used to produce microgel particles in microfluidic chips. Various ionic crosslinking methods for the gelation of charged polymers are discussed, including external gelation via crosslinkers dissolved or dispersed in the oil phase; internal gelation methods using crosslinkers added to the dispersed phase in their non-active forms, such as chelating agents, photo-acid generators, sparingly soluble or slowly hydrolyzing compounds, and methods involving competitive ligand exchange; rapid mixing of polymer and crosslinking streams; and merging polymer and crosslinker droplets. Covalent crosslinking methods using enzymatic oxidation of modified biopolymers, photo-polymerization of crosslinkable monomers or polymers, and thiol-ene “click” reactions are also discussed, as well as methods based on the sol−gel transitions of stimuli responsive polymers triggered by pH or temperature change. In addition to homogeneous microgel particles, the production of structurally heterogeneous particles such as composite hydrogel particles entrapping droplet interface bilayers, core−shell particles, organoids, and Janus particles are also discussed. Microfluidics offers the ability to precisely tune the chemical composition, size, shape, surface morphology, and internal structure of microgels by bringing multiple fluid streams in contact in a highly controlled fashion using versatile channel geometries and flow configurations, and allowing for controlled crosslinking.

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“…The typical example of ionic crosslinking is electrostatic interaction between negatively charged alginate chains and positively charged Ca 2+ ions. Ionic crosslinking in microfluidic channels can be achieved using five different strategies [21]: internal gelation, external gelation, rapid in-droplet mixing (chaotic advection), droplet merging (coalescence) and competitive ligand exchange.…”

Section: Ionic Crosslinking Of Droplets In Microfluidic Channelsmentioning

confidence: 99%

Crosslinking Strategies for Microfluidic Production of Microgels

Chen¹,

Bolognesi²,

Vladisavljević³

2021

Preprint

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This article provides a systematic review of the crosslinking strategies used to produce microgel particles in microfluidic chips. Various ionic crosslinking methods for gelation of charged pol-ymers are discussed, including external gelation via crosslinkers dissolved or dispersed in the oil phase, internal gelation methods using crosslinkers added to the dispersed phase in their non-active forms, such as chelating agents, photo-acid generators, sparingly soluble or slowly hydrolyzing compounds, and methods involving competitive ligand exchange, rapid mixing of polymer and crosslinking streams, and merging polymer and crosslinker droplets. Covalent crosslinking methods using enzymatic oxidation of modified biopolymers, photo-polymerization of crosslinkable monomers or polymers, and thiol-ene “click” reactions are also discussed, as well as the methods based on sol-gel transitions of stimuli responsive polymers triggered by pH or temperature change. In addition to homogeneous microgel particles, the production of structurally heterogeneous particles such as composite hydrogel particles entrapping droplet interface bi-layers, core-shell particles, organoids, and Janus particles are also discussed. Microfluidics offers the ability to precisely tune chemical composition, size, shape, surface morphology, and internal structure of microgels by bringing in contact multiple fluid streams in a highly controlled fashion using versatile channel geometries and flow configurations and allowing controlled crosslinking.

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Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Cited by 20 publications

References 112 publications

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

Crosslinking Strategies for the Microfluidic Production of Microgels

Crosslinking Strategies for Microfluidic Production of Microgels

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