Multimode fibre: Light-sheet microscopy at the tip of a needle

Abstract

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Ultrathin endoscopes based on multicore fibers and adaptive optics: a status review and perspectives

“…While the majority of the initial reports of lensless endoscopes used MMF as the waveguide, [33][34][35][36][37][38][39][40][41][42][43] MCFs can also be used, as can any other waveguide with spatial degrees of freedom. In this section, we will describe the merits of the MCFs that we have designed during our work on lensless endoscopes; in effect, these MCFs have been designed to enhance these merits, which, consequently, may not hold for generic MCFs.…”

Multichannel fiber photometry for mapping axonal terminal activity in a restricted brain region in freely moving mice

“…Recently, researchers reported methods for multimode fiberbased imaging techniques. [47][48][49] However, there are several limitations that prevent the application of these methods to brain tissue. Owing to randomization, thick scattering samples such as brain tissue are not suitable for this method.…”

Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber

“…The lateral resolution of the system was also measured at difference distances away from the fiber facet and remains unchanged until about 300 μm. More details about the change of the 3D point spread function and the field of view (FOV) of a MMF as a function of distance from the fiber facet in previous works on imaging through fiber probes [7,38,39,[44][45][46]. With a range of 300 μm within which the resolution remains unchanged, we believe that imaging of thick samples up to 200-300 μm would be possible.…”