Multimodal Optical Medical Imaging Concepts Based on Optical Coherence Tomography

Leitgeb, Rainer A.; Baumann, Bernhard

doi:10.3389/fphy.2018.00114

Cited by 38 publications

(35 citation statements)

References 187 publications

(115 reference statements)

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“…The quantitative analysis of the iron load internalized into granulocytes using the MRI, ICP-MS and NIRF techniques shows values ( Table 7) that are in accordance with the signal detection limitations as a function of sensitivity, spatial resolution, temporal resolution and the concentration of contrast agent [38,39]. In studies by Shanhua et al [54,86], quantification analysis of the iron load of the SPIONs internalized into granulocytes was performed using the ICP technique, which showed an iron load of 0.1265 pg/cell (0.1265 ppm/106 cell) in the best strategy of internalization using LPS and 5 mg/mL SPIONs.…”

Section: Discussionsupporting

confidence: 54%

“…Isolation purity, viability and yield are important factors for evaluating each method and ensuring cellular integrity [29,[34][35][36].The noninvasive evaluation of acute inflammatory processes makes it possible to analyze molecular and cellular processes in vivo, usually based on targeting specialized cells such as macrophages, lymphocytes or granulocytes to the desired target [37]. Noninvasive imaging techniques that provide structural, functional and molecular information in preclinical and clinical studies include CT, MRI, positron emission tomography (PET), single-photon emission computed tomography (SPETC) and near-infrared fluorescence (NIRF) imaging, among others [38][39][40], each of which has advantages and limitations. The MRI technique offers high spatial resolution but low sensitivity, high cost and long scanning time.…”

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confidence: 99%

“…Thus, a combination of imaging techniques (multimodal imaging) can overcome the disadvantages of each and provide complementary information on the inflammation/infection process. The accurate detection of various pathologies requires multimodal agents that have optical, magnetic, fluorescent and radioactive properties, among others, with at least two such properties in a single probe [38,42,43] that have adequate retention in the place of interest, that present contrast differences between healthy and pathological tissues and that have low cost and easy preparation, all resulting in a multimodal image containing anatomical, functional and metabolic information [16,17,44].One of the possibilities for contrast agents is nanoparticles, which have the potential to be used by various molecular imaging techniques due to their range of magnetic, fluorescent and radiative properties, among others [45]. The most commonly used nanoparticles are metals, Qdots, liposomes, lipid nanoparticles, micelle nanotubes, quantum dots, dendrimers, fluorescent nanoparticles and polymeric nanoparticles [46].…”

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confidence: 99%

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confidence: 99%

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Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

et al. 2020

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This in vitro study aimed to find the best method of granulocyte isolation for subsequent labeling with multimodal nanoparticles (magnetic and fluorescent properties) to enable detection by optical and magnetic resonance imaging (MRI) techniques. The granulocytes were obtained from venous blood samples from 12 healthy volunteers. To achieve high purity and yield, four different methods of granulocyte isolation were evaluated. The isolated granulocytes were labeled with multimodal superparamagnetic iron oxide nanoparticles (M-SPIONs) coated with dextran, and the iron load was evaluated qualitatively and quantitatively by MRI, near-infrared fluorescence (NIRF) and inductively coupled plasma mass spectrometry (ICP-MS). The best method of granulocyte isolation was Percoll with Ficoll, which showed 95.92% purity and 94% viability. After labeling with M-SPIONs, the granulocytes showed 98.0% purity with a yield of 3.5 × 10 6 cells/mL and more than 98.6% viability. The iron-loading value in the labeled granulocytes, as obtained by MRI, was 6.40 ± 0.18 pg/cell. Similar values were found with the ICP-MS and NIRF imaging techniques. Therefore, our study shows that it is possible to isolate granulocytes with high purity and yield and labeling with M-SPIONs provides a high internalized iron load and low toxicity to cells. Therefore, these M-SPION-labeled granulocytes could be a promising candidate for future use in inflammation/infection detection by optical and MRI techniques.Hard-to-reach or occult inflammation and infection processes are clinically challenging. Their clinical diagnoses involve biochemical and radiological examinations, but these methods can produce false negatives [11][12][13][14][15]. To determine the localization, extent and severity of inflammation, imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) can be performed but have limitations in the early stages of the disease, as they are dependent on morphological changes [16][17][18]. Accurate and early diagnosis of inflammation and infection helps reduce mortality and morbidity and increase drug treatment success [19][20][21].Proinflammatory effects help leukocytes migrate from the blood vessel to the site of injury, due to increased vascular permeability through stimulation by cytokines and interleukins. There is an initial accumulation of granulocytes, mainly neutrophils, in the inflamed area and later of lymphocytes and macrophages [22][23][24]. Granulocytes are the most abundant subset of leukocytes [25] and are produced in the bone marrow, originating from myeloid precursor cells and undergoing a maturing process before they are able to perform their functions, mainly phagocytosis [25,26]. They differ from other cells in that they have granules with proteolytic enzymes that fight microorganisms or other inflammatory agents [25,27]. Granulocytes are considered frontline cells in inflammation and infection processes [28]. In many cases, to detect inflammation/infection, it is necessary to label isolated granulocyte...

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Section: Discussionsupporting

confidence: 54%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

et al. 2020

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“…To facilitate ROI relocation, CMI also aims at setting up hardware-fused hybrid setups that inherently co-localize the same ROI due to their joint coordination system. Apart from well-known commercially available PHI scanners (such as PET/CT, SPECT/CT, or PET/MRI), as described in section State-of-the-Art, examples include (i) a variety of hybrid AFM and FM setups [30,171,172], (ii) first implementations of integrated EM-FM setups [111,173], (iii) several combined setups of OCT with photoacoustics or non-linear microscopy [174], and (iv) diverse hardwarebased approaches to combine OI techniques with CT or MRI [175]. Nevertheless, in certain cases, relocation across single modality systems using cross-platform transport beds for CMI may provide superior performance compared to hybrid systems where compromises may have been made in the integration process.…”

Section: Novel CMI Pipelinesmentioning

confidence: 99%

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

et al. 2020

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Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging

Tuchina

Meerovich

Sindeeva

et al. 2020

Journal of Biophotonics

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Skin optical clearing effect ex vivo and in vivo was achieved by topical application of low molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has not been explored before. By using collimated transmittance the diffusion coefficients of three clinically used magnetic resonance contrast agents, that is Gadovist, Magnevist and Dotarem as well as X-ray contrast agent Visipaque in mouse skin were determined ex vivo as (4.29 ± 0.39) × 10 −7 cm 2 / s, (5.00 ± 0.72) × 10 −7 cm 2 /s, (3.72 ± 0.67) × 10 −7 cm 2 /s and (1.64 ± 0.18) × 10 −7 cm 2 /s, respectively. The application of gadobutrol (Gadovist) resulted in

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Multimodal Optical Medical Imaging Concepts Based on Optical Coherence Tomography

Cited by 38 publications

References 187 publications

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging

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