Multimodal neural probes for combined optogenetics and electrophysiology

Xu, Ke; Zou, Liang; Fang, Ying

doi:10.1016/j.isci.2021.103612

Cited by 14 publications

(10 citation statements)

References 100 publications

(196 reference statements)

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“…Optogenetics combines genetic and optical methods to control cell-type specific activation or inhibition of neural activities with light, which brings specific changes of electrical signals. 175 Stark et al thinned 200 μm diameter optical fibers down to a diameter of 60−70 μm by sharpening the fiber tips with chemical etching, and each thin fiber was integrated with a light-emitting diode (LED) for local light transmission and light stimulation. 176 Then, in the combination of optogenetics and electrophysiology, each diode fiber assembly was attached to the silicon probe with a gap of only 30−40 μm.…”

Section: ■ New Electrochemical Devicesmentioning

confidence: 99%

“…Recently, as an emerging technology, the development of multimodal neural probes that combine optogenetics and electrophysiology has provided a powerful tool for dissecting neural circuit functions and understanding brain diseases. Optogenetics combines genetic and optical methods to control cell-type specific activation or inhibition of neural activities with light, which brings specific changes of electrical signals . Stark et al thinned 200 μm diameter optical fibers down to a diameter of 60–70 μm by sharpening the fiber tips with chemical etching, and each thin fiber was integrated with a light-emitting diode (LED) for local light transmission and light stimulation .…”

Section: New Electrochemical Devicesmentioning

confidence: 99%

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In Vivo Electrochemical Biosensors: Recent Advances in Molecular Design, Electrode Materials, and Electrochemical Devices

et al. 2023

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Electrochemical biosensors provide powerful tools for dissecting the dynamically changing neurochemical signals in the living brain, which contribute to the insight into the physiological and pathological processes of the brain, due to their high spatial and temporal resolutions. Recent advances in the integration of in vivo electrochemical sensors with cross-disciplinary advances have reinvigorated the development of in vivo sensors with even better performance. In this Review, we summarize the recent advances in molecular design, electrode materials, and electrochemical devices for in vivo electrochemical sensors from molecular to macroscopic dimensions, highlighting the methods to obtain high performance for fulfilling the requirements for determination in the complex brain through flexible and smart design of molecules, materials, and devices. Also, we look forward to the development of next-generation in vivo electrochemical biosensors.

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Section: ■ New Electrochemical Devicesmentioning

confidence: 99%

Section: New Electrochemical Devicesmentioning

confidence: 99%

In Vivo Electrochemical Biosensors: Recent Advances in Molecular Design, Electrode Materials, and Electrochemical Devices

et al. 2023

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“…Cell activation causes either depolarization or hyperpolarization. 70 Quantitative behavioral analysis, electrical recordings, and fluorescent indicators control optical readouts. The halorhodopsins (NpHR) and channelrhodopsins (ChR2) were discovered as new microbial opsin families that transport various ions across the membrane in response to light.…”

Section: Optogeneticsmentioning

confidence: 99%

“…It entails using a virus with a light-sensitive gene to transfer the protein to the cell’s surface. Cell activation causes either depolarization or hyperpolarization . Quantitative behavioral analysis, electrical recordings, and fluorescent indicators control optical readouts.…”

Section: Optical Imaging Using Chemical and Genetically Modified Vira...mentioning

confidence: 99%

In Vivo and 3D Imaging Technique(s) for Spatiotemporal Mapping of Pathological Events in Experimental Model(s) of Spinal Cord Injury

Goyal

Kumar

2023

ACS Chem. Neurosci.

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Endothelial damage, astrogliosis, microgliosis, and neuronal degeneration are the most common events after spinal cord injury (SCI). Studies highlighted that studying the spatiotemporal profile of these events might provide a deeper understanding of the pathophysiology of SCI. For imaging of these events, available conventional techniques such as 2-dimensional histology and immunohistochemistry (IHC) are well established and frequently used to visualize and detect the altered expression of the protein of interest involved in these events. However, the technique requires the physical sectioning of the tissue, and results are also open to misinterpretation. Currently, researchers are focusing more attention toward the advanced tools for imaging the spinal cord’s various physiological and pathological parameters. The tools include two-photon imaging, light sheet fluorescence microscopy, in vivo imaging system with fluorescent probes, and in vivo chemical and fluorescent protein-expressing viral-tracers. These techniques outperform the limitations associated with conventional techniques in various aspects, such as optical sectioning of tissue, 3D reconstructed imaging, and imaging of particular planes of interest. In addition to this, these techniques are minimally invasive and less time-consuming. In this review, we will discuss the various advanced imaging methodologies that will evolve in the future to explore the fundamental mechanisms after SCI.

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“…To unravel the causal relationships between neural circuit activity and brain function, it is necessary to simultaneously record and manipulate the activity of specific types of neurons in the brain. Over the past few years, a variety of multifunctional neural probes with integrated recording and stimulating units have been developed [13][14][15][16] . Multifunctional neural probes provide a powerful platform to simultaneously monitor the activities of neurons and their responses to well-controlled stimuli [17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Recent developments in multifunctional neural probes for simultaneous neural recording and modulation

Wang

Fang

2023

Microsyst Nanoeng

Self Cite

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Neural probes are among the most widely applied tools for studying neural circuit functions and treating neurological disorders. Given the complexity of the nervous system, it is highly desirable to monitor and modulate neural activities simultaneously at the cellular scale. In this review, we provide an overview of recent developments in multifunctional neural probes that allow simultaneous neural activity recording and modulation through different modalities, including chemical, electrical, and optical stimulation. We will focus on the material and structural design of multifunctional neural probes and their interfaces with neural tissues. Finally, future challenges and prospects of multifunctional neural probes will be discussed.

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Multimodal neural probes for combined optogenetics and electrophysiology

Cited by 14 publications

References 100 publications

In Vivo Electrochemical Biosensors: Recent Advances in Molecular Design, Electrode Materials, and Electrochemical Devices

In Vivo Electrochemical Biosensors: Recent Advances in Molecular Design, Electrode Materials, and Electrochemical Devices

In Vivo and 3D Imaging Technique(s) for Spatiotemporal Mapping of Pathological Events in Experimental Model(s) of Spinal Cord Injury

Recent developments in multifunctional neural probes for simultaneous neural recording and modulation

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