Multimodal mapping of human skin

Heuke, Sandro; Vogler, Nadine; Meyer, Tobias; Akimov, D. A.; Kluschke, Franziska; Röwert‐Huber, Hans‐Joachim; Lademann, Juergen; Dietzek, Benjamin; Popp, Jürgen

doi:10.1111/bjd.12427

Cited by 51 publications

(44 citation statements)

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“…[264] Evaluation of the multiphoton images showed that it is possible to distinguish between epidermis,d ermis,a nd subcutis by using ac ombination of all three modalities. [264] Evaluation of the multiphoton images showed that it is possible to distinguish between epidermis,d ermis,a nd subcutis by using ac ombination of all three modalities.…”

Section: Coherent Raman Scattering In Tissue Imagingmentioning

confidence: 99%

“…Heuke et al used ac ombination of CARS,S HG,a nd TPEF microscopy to gain ac omprehensive overview of skin samples from 32 individuals. [264] Evaluation of the multiphoton images showed that it is possible to distinguish between epidermis,d ermis,a nd subcutis by using ac ombination of all three modalities. Furthermore,i tw as possible to identify tissue structures,s uch as hair follicles, sebaceous and sweat glands,a nd blood vessels.Being able to acquire information on the skin in al abel-free manner is not only of interest for histopathological diagnosis in clinics,b ut also for the pharmaceutical and cosmetics industries,w hich have significant interest in investigating and understanding various properties, such as the penetration and absorption of drugs and creams into tissues.…”

Section: Coherent Raman Scattering In Tissue Imagingmentioning

confidence: 99%

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Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and tissues by Raman microspectroscopy represents a nondestructive and label-free approach. All components of cells or tissues contribute to the Raman signals, giving rise to complex spectral signatures. Resonance Raman scattering and surface-enhanced Raman scattering can be used to enhance the signals and reduce the spectral complexity. Raman-active labels can be introduced to increase specificity and multimodality. In addition, nonlinear coherent Raman scattering methods offer higher sensitivities, which enable the rapid imaging of larger sampling areas. Finally, fiber-based imaging techniques pave the way towards in vivo applications of Raman spectroscopy. This Review summarizes the basic principles behind medical Raman imaging and its progress since 2012.

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Section: Coherent Raman Scattering In Tissue Imagingmentioning

confidence: 99%

Section: Coherent Raman Scattering In Tissue Imagingmentioning

confidence: 99%

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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“…Many other conditions also lead to architectural changes that change the mechanical properties and appearances of skin, where accumulation of mucopolysaccharides leads to swelling and inelasticity in mucinosis and myxoedema, or degeneration of key components such as fibrillin 5 can lead to elastolysis in conditions such as Cutis laxa .…”

Section: Pathology Differencesmentioning

confidence: 99%

The dynamic anatomy and patterning of skin

Wong

Geyer

Weninger

et al. 2015

Experimental Dermatology

298

201

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The skin is often viewed as a static barrier that protects the body from the outside world. Emphasis on studying the skin's architecture and biomechanics in the context of restoring skin movement and function is often ignored. It is fundamentally important that if skin is to be modelled or developed, we do not only focus on the biology of skin but also aim to understand its mechanical properties and structure in living dynamic tissue. In this review, we describe the architecture of skin and patterning seen in skin as viewed from a surgical perspective and highlight aspects of the microanatomy that have never fully been realized and provide evidence or concepts that support the importance of studying living skin's dynamic behaviour. We highlight how the structure of the skin has evolved to allow the body dynamic form and function, and how injury, disease or ageing results in a dramatic changes to the microarchitecture and changes physical characteristics of skin. Therefore, appreciating the dynamic microanatomy of skin from the deep fascia through to the skin surface is vitally important from a dermatological and surgical perspective. This focus provides an alternative perspective and approach to addressing skin pathologies and skin ageing.

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“…The high imaging speed of CARS microscopy has recently also been exploited to monitor structural changes of sebaceous glands during sebum secretion [129]. In order to compare healthy skin and pathological changes, a number of experiments have been performed mapping skin with multimodal approaches including CARS microscopy [129][130][131][132]. Another example for lipid rich structures which are well suited for non-linear Raman imaging are atherosclerotic deposits.…”

Section: Tissue Experimentsmentioning

confidence: 99%