Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view

Lai, Chenting; Messerschmidt, Bernhard; Flämig, Sven; Reichwald, Karl; Bae, Hyeonsoo; Calvarese, Matteo; Meyer, Tobias; Schmitt, Michael; Hoffmann, Franziska; Guntinas‐Lichius, Orlando; Popp, Jürgen

doi:10.1117/12.2621692

Cited by 1 publication

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“…As illustrated by the optical path in Fig. 1(b), 23 excitation beams are delivered together from a hollow-core fiber (PMC-C-R&D, GLOphotonics), and first collimated by a custom-made Galilean telescope (f=60 mm) then deflected by a Galvo scanner (Cambridge Technology, 6210H). A long-pass filter F1 (FELH0750, Thorlabs) removes four-wave mixing noise from the delivery fiber.…”

Section: Design Concept and Simulationmentioning

confidence: 99%

“…varying the energy per pulse in a range from 20 to 500 nJ at a fixed repetition rate of 190 kHz. The maximum limit around 500 nJ was chosen to avoid possible damages in the endomicroscope’s optics and because an effective ablation at lower energy per pulse was already proven with several tests on biological samples 23 . Similar laser parameters were also used in previous works 6 .…”

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

“…The maximum limit around 500 nJ was chosen to avoid possible damages in the endomicroscope’s optics and because an effective ablation at lower energy per pulse was already proven with several tests on biological samples. 23 Similar laser parameters were also used in previous works. 6 The pulse temporal shape was retrieved directly from the

fit in the A.P.E.…”

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

“…Some of the results in this article have been previously reported in SPIE conference proceedings. 22 , 23 , 40 The authors declare no commercial conflicts of interest related to this article.…”

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confidence: 99%

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Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Lai,

Calvarese,

Reichwald

et al. 2023

J. Biomed. Opt.

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.SignificanceConventional diagnosis of laryngeal cancer is normally made by a combination of endoscopic examination, a subsequent biopsy, and histopathology, but this requires several days and unnecessary biopsies can increase pathologist workload. Nonlinear imaging implemented through endoscopy can shorten this diagnosis time, and localize the margin of the cancerous area with high resolution.AimDevelop a rigid endomicroscope for the head and neck region, aiming for in-vivo multimodal imaging with a large field of view (FOV) and tissue ablation.ApproachThree nonlinear imaging modalities, which are coherent anti-Stokes Raman scattering, two-photon excitation fluorescence, and second harmonic generation, as well as the single photon fluorescence of indocyanine green, are applied for multimodal endomicroscopic imaging. High-energy femtosecond laser pulses are transmitted for tissue ablation.ResultsThis endomicroscopic system consists of two major parts, one is the rigid endomicroscopic tube 250 mm in length and 6 mm in diameter, and the other is the scan-head (10 × 12 × 6 cm3 in size) for quasi-static scanning imaging. The final multimodal image accomplishes a maximum FOV up to 650 μm, and a resolution of 1 μm is achieved over 560 μm FOV. The optics can easily guide sub-picosecond pulses for ablation.ConclusionsThe system exhibits large potential for helping real-time tissue diagnosis in surgery, by providing histological tissue information with a large FOV and high resolution, label-free. By guiding high-energy fs laser pulses, the system is even able to remove suspicious tissue areas, as has been shown for thin tissue sections in this study.

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Section: Design Concept and Simulationmentioning

confidence: 99%

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

fit in the A.P.E.…”

Section: Preliminary Endomicroscopic Imaging and Fs Laser Ablation Testsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Lai,

Calvarese,

Reichwald

et al. 2023

J. Biomed. Opt.

Self Cite

View full text Add to dashboard Cite

show abstract

Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view

Cited by 1 publication

References 0 publications

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

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