Multimodal full-field optical coherence tomography on biological tissue: toward all optical digital pathology

Harms, Fabrice; Dalimier, Eugénie; Vermeulen, Pierre; Fragola, Alexandra; Boccara, Albert Claude

doi:10.1117/12.908459

Cited by 15 publications

(12 citation statements)

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“…To increase the accuracy of diagnosis of tumors where cell density measurement is necessary for grading, perspectives for the technique include development of a multimodal system (Harms et al 2012) to allow simultaneous co-localized acquisition of FF-OCT and fluorescence images. Use of this multimodal setup may be of interest in further exploration of the possibility of diagnosis and tumor grading direct from optical images, as the fluorescence channel images would show cell nuclei.…”

Section: Discussionmentioning

confidence: 99%

Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

Assayag

Grieve

Devaux

et al. 2013

NeuroImage: Clinical

161

114

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A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of full-field optical coherence tomography (FF-OCT) in brain tumor diagnosis.FF-OCT captures en face slices of tissue samples at 1 μm resolution in 3D to a penetration depth of around 200 μm. A 1 cm2 specimen is scanned at a single depth and processed in about 5 min. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, which makes it particularly well suited to medical imaging applications.Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, low-grade and high-grade gliomas, and choroid plexus papilloma were imaged. A subpopulation of neurons, myelin fibers and CNS vasculature were clearly identified. Cortex could be discriminated from white matter, but individual glial cells such as astrocytes (normal or reactive) or oligodendrocytes were not observable.This study reports for the first time on the feasibility of using FF-OCT in a real-time manner as a label-free non-invasive imaging technique in an intraoperative neurosurgical clinical setting to assess tumorous glial and epileptic margins.

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Section: Discussionmentioning

confidence: 99%

Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

Assayag

Grieve

Devaux

et al. 2013

NeuroImage: Clinical

161

114

View full text Add to dashboard Cite

show abstract

“…acriflavin, hematoxylin, etc.,) to distinguish tumor cells from inflammatory cells; and (4) exploring multimodal imaging approaches (e.g. combining FFOCT with fluorescence[18] etc.). In addition, imaging at higher magnification (and with a higher numerical aperture objective) might help better distinguish between adenocarcinoma cells and histiocytes based on cytologic features.…”

Section: Discussionmentioning

confidence: 99%

Full-field optical coherence tomography for the analysis of fresh unstained human lobectomy specimens

Jain

Narula

Salamoon

et al. 2013

Journal of Pathology Informatics

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Background:Full-field optical coherence tomography (FFOCT) is a real-time imaging technique that generates high-resolution three-dimensional tomographic images from unprocessed and unstained tissues. Lack of tissue processing and associated artifacts, along with the ability to generate large-field images quickly, warrants its exploration as an alternative diagnostic tool.Materials and Methods:One section each from the tumor and from adjacent non-neoplastic tissue was collected from 13 human lobectomy specimens. They were imaged fresh with FFOCT and then submitted for routine histopathology. Two blinded pathologists independently rendered diagnoses based on FFOCT images.Results:Normal lung architecture (alveoli, bronchi, pleura and blood vessels) was readily identified with FFOCT. Using FFOCT images alone, the study pathologists were able to correctly identify all tumor specimens and in many cases, the histological subtype of tumor (e.g., adenocarcinomas with various patterns). However, benign diagnosis was provided with high confidence in roughly half the tumor-free specimens (others were diagnosed as equivocal or false positive). Further analysis of these images revealed two major confounding features: (a) Extensive lung collapse and (b) presence of smoker's macrophages. On a closer inspection, however, the smoker's macrophages could often be identified as distinct from tumor cells based on their relative location in the alveoli, size and presence of anthracosis. We posit that greater pathologist experience, complemented with morphometric analysis and color-coding of image components, may help minimize the contribution of these confounders in the future.Conclusion:Our study provides evidence for the potential utility of FFOCT in identifying and differentiating lung tumors from non-neoplastic lung tissue. We foresee its potential as an adjunct to intra-surgical frozen section analysis for margin assessment, especially in limited lung resections.

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“…Many such multimodal setups have been developed with both scanning OCT approaches [5,147] and full-field approaches, using either a flip mirror to switch from one modality to the other [18,148], or a simultaneous approach [41,42]. If all these multimodal approaches share a common interest, scanning OCT approaches are more easily combined with scanning fluorescence approaches, such as confocal or two photon microscopies, whereas FF-OCT systems have been developed in combination with widefield fluorescence techniques, such as structured illumination microscopy (SIM).…”

Section: Full-field Oct and Fluorescencementioning

confidence: 99%

Full-Field Optical Coherence Tomography as a Diagnosis Tool: Recent Progress with Multimodal Imaging

et al. 2017

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Full-field optical coherence tomography (FF-OCT) is a variant of OCT that is able to register 2D en face views of scattering samples at a given depth. Thanks to its superior resolution, it can quickly reveal information similar to histology without the need to physically section the sample. Sensitivity and specificity levels of diagnosis performed with FF-OCT are 80% to 95% of the equivalent histological diagnosis performances and could therefore benefit from improvement. Therefore, multimodal systems have been designed to increase the diagnostic performance of FF-OCT. In this paper, we will discuss which contrasts can be measured with such multimodal systems in the context of ex vivo biological tissue examination. We will particularly emphasize three multimodal combinations to measure the tissue mechanics, dynamics, and molecular content respectively.

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Multimodal full-field optical coherence tomography on biological tissue: toward all optical digital pathology

Cited by 15 publications

References 0 publications

Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography

Full-field optical coherence tomography for the analysis of fresh unstained human lobectomy specimens

Full-Field Optical Coherence Tomography as a Diagnosis Tool: Recent Progress with Multimodal Imaging

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