Full-Field Optical Coherence Tomography as a Diagnosis Tool: Recent Progress with Multimodal Imaging

Abstract

Full-field optical coherence tomography (FF-OCT) is a variant of OCT that is able to register 2D en face views of scattering samples at a given depth. Thanks to its superior resolution, it can quickly reveal information similar to histology without the need to physically section the sample. Sensitivity and specificity levels of diagnosis performed with FF-OCT are 80% to 95% of the equivalent histological diagnosis performances and could therefore benefit from improvement. Therefore, multimodal systems have been designed to increase the diagnostic performance of FF-OCT. In this paper, we will discuss which contrasts can be measured with such multimodal systems in the context of ex vivo biological tissue examination. We will particularly emphasize three multimodal combinations to measure the tissue mechanics, dynamics, and molecular content respectively.

Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

“…6,18 Dynamic FFOCT is also highly sensitive to membrane flickering and cell surface remodeling, as observed in red blood cells and in lymphocytes. 34 We expect the dynamic FFOCT signal to be high in cytoplasm where many organelles are trafficking, in red blood cells, and in photoreceptor IS which are full of mitochondria. The dynamic FFOCT signal in the photoreceptor OS is not so significant but might be caused by membrane fluctuations since membrane density is important and no cytoskeleton is present to prevent thermal fluctuations.…”

Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT

“…Recently, a multimodal setup ( Fig. 1(a)) was proposed to combine static and dynamic FFOCT with fluorescence microscopy [40]. This setup can be used to confirm the FFOCT identity of specific cell types or behaviours via comparison with the fluorescent signal.…”

Sub-4-micron full-field optical coherence tomography on a budget

“…Our approach was to implement full-field optical coherence tomography (FF-OCT), or optical coherence microscopy (OCM) [9][10][11][12] using a time domain (TD) set-up -this configuration avoids expensive galvo-mirror scanners [3][4][5]13] and swept sources [14][15][16]. The use of a motorized translation stage and LED sources instead of more expensive super-luminescent diode (SLED) or halogen sources was a key driver in our cost-cutting.…”