Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

Thouvenin, Olivier; Boccara, Claude; Fink, Mathias; Sahel, J; Pâques, Michel; Grieve, Kate

doi:10.1167/iovs.17-22375

Cited by 41 publications

(46 citation statements)

References 43 publications

(60 reference statements)

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“…5g). The AO-OCT results are consistent with previous observations showing that averaging per se leads to reduction in speckle contrast in in vivo imaging 24,25 . This reduction was hypothesized to arise from the movements of subcellular organelles whose scattering gives rise to speckle.…”

Section: In Vivo Application Of Apm-ao-oct Reduces Speckle Efficientlsupporting

confidence: 91%

“…Recently, it was also found that averaging of multiple, precisely aligned volumes for in vivo OCT imaging could reduce speckle noise and reveal novel cellular scale structure 24,25 . It was hypothesized that such averaging is effective because of the random movement of sub-PSF size scattering elements in cells.…”

Section: Comparison With Similar Methods Of Speckle Reductionmentioning

confidence: 99%

“…of scatters was responsible for varying the speckle pattern between frames 24,25 . Because this latter method relies on time-dependent variation in the sample microstructure, it is inherently passive and dependent on the underlying time course of the mobile scatters.…”

mentioning

confidence: 99%

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Aperture Phase Modulation with Adaptive Optics: A Novel Approach for Speckle Reduction and Structure Extraction in Optical Coherence Tomography

Zhang

Manna

Miller

et al. 2018

Preprint

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Speckle is an inevitable consequence of the use of coherent light in optical coherence tomography (OCT), and often acts as noise that obscures micro-structures of biological tissue. We here present a novel method of suppressing speckle noise intrinsically compatible with adaptive optics (AO) in OCT system: by modulating the phase inside the imaging system pupil aperture with a segmented deformable mirror, thus producing minor perturbations in the point spread function (PSF) to create un-correlated speckle pattern between B-scans, and further averaging to wash out the speckle but maintain the structures. It is a well-controlled and universal method which can efficiently determine the optimal range of phase modulation that minimizing speckle noise while maximizing image resolution and signal strength for different systems and/or samples. As an active method, its effectiveness and efficiency were demonstrated by both ex-vivo non-biological and in-vivo biological applications. Introduction:Speckle is a long-standing issue in all imaging technologies that use coherent light sources 1-4 , arised from the interference between light scattered by a random distributed scatters inside the system point-spread function (PSF), and observed as voxel-to-voxel intensity fluctuations in the image 5, 6 . Although speckle is a potential useful information about the dynamics of sample microstructure, in most applications it acts as a major noise source that degrades image quality. Optical coherence tomography (OCT) is a volumetric imaging technology developed in 1991 7 . It has been soon adopted in many biomedical applications 8-12 . However, as a method dependent on the coherent properties of light, OCT images suffer from speckle noise [13][14][15] .Many approaches have been taken to suppress speckle, including generation of multiple images by various means with uncorrelated speckle patterns, followed by averaging [16][17][18][19] . A weakness of these methods is that the number of uncorrelated speckle patterns that can be created is typically small, limiting speckle suppression by averaging. Speckle reduction methods using digital post-processing have also been proposed 20-23 . However, digital post-processing usually reduces speckle by spatial averaging or filtering, which necessarily reduces image resolution. Recently, it was shown that simple averaging of suitably numerous, well aligned images can reduce speckle for in vivo imaging, and it was hypothesized that subcellular motility Fig. 1 | System setup (photo in SI: Fig. S2) and the geometry of the segmented deformable mirror. a, OCT sample arm (Inset: (i) -Photo of the DM; (ii) -setup for USAF 1951 resolution test target; (iii)covariance analysis of the random segments pistons). b, Mirror configuration -Flat. c, Mirror Configuration -mirror segments with random displacements [SI: Video_S1]. d, Histogram of the mirror displacements for 100 mirror configurations in which the displacement range was 1m (0  0.5 m); Abbreviations: L#: Lens, VL: variable focus length liquid lens, ...

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Section: In Vivo Application Of Apm-ao-oct Reduces Speckle Efficientlsupporting

confidence: 91%

Section: Comparison With Similar Methods Of Speckle Reductionmentioning

confidence: 99%

See 1 more Smart Citation

Aperture Phase Modulation with Adaptive Optics: A Novel Approach for Speckle Reduction and Structure Extraction in Optical Coherence Tomography

Zhang

Manna

Miller

et al. 2018

Preprint

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“…Recently, new multimodal techniques, based either on OCT or FFOCT, have enabled the study not only of the static 3D morphology of a sample but also of the dynamics of its scatterers [7,8] by measuring phase-sensitive temporal fluctuations of the backscattered light. In ex-vivo fresh unfixed tissues, these dynamic techniques reveal subcellular structures that are very weak backscatterers and provide additional contrast based on their intracellular motility [9,10]. FFOCT multimodal techniques can also detect collective subcellular motion of scatterers resulting from either static deformations or propagating elastic waves for mapping elastic properties or flow of cells in capillaries [11,12].…”

Section: Introductionmentioning

confidence: 99%

Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT

Scholler¹,

Mazlin²,

Thouvenin³

et al. 2019

Biomed. Opt. Express

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et al.. Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT. Abstract:We describe recent technological progress in multimodal en face full-field optical coherence tomography that has allowed detection of slow and fast dynamic processes in the eye. We show that by combining static, dynamic and fluorescence contrasts we can achieve label-free high-resolution imaging of the retina and anterior eye with temporal resolution from milliseconds to several hours, allowing us to probe biological activity at subcellular scales inside 3D bulk tissue. Our setups combine high lateral resolution over a large field of view with acquisition at several hundreds of frames per second which make it a promising tool for clinical applications and biomedical studies. Its contactless and non-destructive nature is shown to be effective for both following in vitro sample evolution over long periods of time and for imaging of the human eye in vivo.

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“…Recently, a novel contrast mechanism has been exploited by measuring temporal fluctuations of the backscattered light in a technique called dynamic full field OCT (D-FFOCT) [7]. In ex vivo fresh tissues, these dynamic measurements reveal subcellular structures that are very weak backscatterers and provide contrast based on their intracellular motility [8,9]. A similar technique is used in regular OCT for retinal angiography called OCTA where speckle variance is analyzed on several B-Scans (typically 8 frames) to produce binary images of the retinal vasculature [10].…”

Section: Introductionmentioning

confidence: 99%

Motion artifact removal and signal enhancement to achieve in vivo dynamic full field OCT

Scholler

2019

Opt. Express

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We present a filtering procedure based on singular value decomposition to remove artifacts arising from sample motion during dynamic full field OCT acquisitions. The presented method succeeded in removing artifacts created by environmental noise from data acquired in a clinical setting, including in vivo data. Moreover, we report on a new method based on using the cumulative sum to compute dynamic images from raw signals, leading to a higher signal to noise ratio, and thus enabling dynamic imaging deeper in tissues. * https://www.jscholler.com

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Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

Cited by 41 publications

References 43 publications

Aperture Phase Modulation with Adaptive Optics: A Novel Approach for Speckle Reduction and Structure Extraction in Optical Coherence Tomography

Aperture Phase Modulation with Adaptive Optics: A Novel Approach for Speckle Reduction and Structure Extraction in Optical Coherence Tomography

Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT

Motion artifact removal and signal enhancement to achieve in vivo dynamic full field OCT

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