Multimodal discrimination of immune cells using a combination of Raman spectroscopy and digital holographic microscopy

McReynolds, Naomi; Cooke, Fiona G. M.; Chen, Mingzhou; Powis, Simon J.; Dholakia, Kishan

doi:10.1038/srep43631

Cited by 47 publications

(32 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also requires very low light intensity, thus rendering the approach free from phototoxicity. It has been demonstrated that RS and DHM can be used simultaneously to provide a morphological and biochemical analysis of cells [ 58 ] and it can be applied to living white blood cells [ 59 ]. The phase map can provide information on the shape, dimensions and presence of cell defects that can be correlated with the biochemical information provided by single-point Raman spectra of the analyzed cells [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Raman Microscopy: Progress in Research on Cancer Cell Sensing

Satheeshkumar

Managò

Luca

2020

Sensors

View full text Add to dashboard Cite

In the last decade, Raman Spectroscopy (RS) was demonstrated to be a label-free, non-invasive and non-destructive optical spectroscopy allowing the improvement in diagnostic accuracy in cancer and analytical assessment for cell sensing. This review discusses how Raman spectra can lead to a deeper molecular understanding of the biochemical changes in cancer cells in comparison to non-cancer cells, analyzing two key examples, leukemia and breast cancer. The reported Raman results provide information on cancer progression and allow the identification, classification, and follow-up after chemotherapy treatments of the cancer cells from the liquid biopsy. The key obstacles for RS applications in cancer cell diagnosis, including quality, objectivity, number of cells and velocity of the analysis, are considered. The use of multivariant analysis, such as principal component analysis (PCA) and linear discriminate analysis (LDA), for an automatic and objective assessment without any specialized knowledge of spectroscopy is presented. Raman imaging for cancer cell mapping is shown and its advantages for routine clinical pathology practice and live cell imaging, compared to single-point spectral analysis, are debated. Additionally, the combination of RS with microfluidic devices and high-throughput screening for improving the velocity and the number of cells analyzed are also discussed. Finally, the combination of the Raman microscopy (RM) with other imaging modalities, for complete visualization and characterization of the cells, is described.

show abstract

Section: Discussionmentioning

confidence: 99%

Raman Microscopy: Progress in Research on Cancer Cell Sensing

Satheeshkumar

Managò

Luca

2020

Sensors

View full text Add to dashboard Cite

show abstract

“…That is true for standardized and reproducible expansion of defined immune cell preparations as well as for estimates and methods of measuring specific functionalities of an expanded immune cell population against tumor cells, infections, or inflammations. Moreover, there are also missing generally available methods and techniques for fast and precise measurement of homogeneity of a cell population, of characteristics of sub populations, and single cells [10][11][12][13]. All these aspects appear to be widely responsible for the limited progress by these very promising new therapies.…”

Section: Requirements and Existent Challenges In Producing Immune Celmentioning

confidence: 99%

“…However, the combination of this method with sophisticated software programs with in-depth analyses tools can lead to sharper, high-resolution Raman spectra enabling differentiating looks onto cells enabling subtype identification, quantification, analysis of functional status, etc. [10][11][12][13].…”

Section: Newer Techniques For Characterization and Production Of Immumentioning

confidence: 99%

Landscape of Manufacturing Process of ATMP Cell Therapy Products for Unmet Clinical Needs

Pörtner¹,

Parida²,

Schaffer³

et al. 2018

Stem Cells in Clinical Practice and Tissue Engineering

View full text Add to dashboard Cite

Immune cell therapies have been studied in numerous clinical trials using Advanced Therapy Medicinal Products (ATMP) against a number of diseases having no or inadequate alternative therapies available, for example, various cancer types, cerebral stroke, cardiac infarction, severe autoimmune disorders, or chronic infections. Despite the enormous number of positive observation in ex vivo or animal studies, convincing results in clinical studies remain scanty. The chapter presents a survey and reveals that the manufacturing of immune cells especially for clinical trials is until today primarily performed using archaic, scarcely controlled, and incomparable processes and methods. A deeper characterization of ex vivo expanded immune cells is urgently needed not only on the level of a few receptors and ligands on the cell surface but also with respect to the ever-contained subtypes in an expanded immune cell population, the pattern of secreted effector molecules, and their amounts over time and influences from in vivo components on them.

show abstract

“…Previous work focusing on the label-free holographic analysis of leukocytes analyzed either cells isolated by Ficoll density centrifugation or cells in culture. [7][8][9][10][11][12][13] Central laboratory applications require integrated workflows for a high-throughput analysis. The selective lysis of erythrocytes provides a rapid method for the isolation of leukocytes from whole blood and is widely used in commercial hematology analyzers and fluorescence flow cytometers.…”

Section: Introductionmentioning

confidence: 99%

Impact of sample preparation on holographic imaging of leukocytes

et al. 2019

View full text Add to dashboard Cite

The label-free analysis of leukocytes by holographic microscopy has the potential to overcome the extensive labeling procedures of conventional analysis by automated hematology analyzers and manual blood smear analysis. For the enrichment of leukocytes, selective lysis of erythrocytes is applied in flow cytometry to minimize the background. In the case of quantitative phase measurements, lysis procedures may affect the morphology of leukocytes inducing refractive index changes, which can deteriorate the specificity and sensitivity of the method. To study the effects of selective erythrocyte lysis on phase measurements of leukocytes, we compared two widespread methods: lysis by hypotonic shock followed by magnetic depletion of erythrocytes and lysis with a commercial lysis buffer containing ammonium chloride and potassium bicarbonate. We observed that lymphocytes were less affected than granulocytes and monocytes by both methods and that the intracellular changes increased with increasing incubation times. These findings indicate a significant effect of erythrocyte lysis on leukocytes and need to be studied for future hematology applications. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Multimodal discrimination of immune cells using a combination of Raman spectroscopy and digital holographic microscopy

Cited by 47 publications

References 47 publications

Raman Microscopy: Progress in Research on Cancer Cell Sensing

Raman Microscopy: Progress in Research on Cancer Cell Sensing

Landscape of Manufacturing Process of ATMP Cell Therapy Products for Unmet Clinical Needs

Impact of sample preparation on holographic imaging of leukocytes

Contact Info

Product

Resources

About