Multimodal confocal microscopy for diagnosing nonmelanoma skin cancers

Al-Arashi, Munir; Salomatina, Elena; Yaroslavsky, Anna N.

doi:10.1002/lsm.20578

Cited by 68 publications

(74 citation statements)

References 21 publications

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“…It is approved for human use and does not affect OCT imaging because the MB absorption peaks (618 nm and 668 nm) do not overlap with the wavelength (1,325 nm) used for OCT. Furthermore, this dye has been shown to have similar staining pattern to hematoxylin and eosin (H&E) in histopathology (31)(32)(33), which simplifies morphologic analysis of the images by clinical personnel. Commercially available MB (MB 1% injection, USP, American Regent Laboratories, Inc.) was purchased for this study.…”

Section: Contrast Agentmentioning

confidence: 99%

Polarization-Sensitive Multimodal Imaging for Detecting Breast Cancer

et al. 2014

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Intraoperative delineation of breast cancer is a significant problem in surgical oncology. A reliable method for demarcation of malignant breast tissue during surgery would reduce the re-excision rate due to positive margins. We present a novel method of identifying breast cancer margins using combined dye-enhanced wide-field fluorescence polarization imaging for en face cancer margins and polarization-sensitive (PS) optical coherence tomography (OCT) for cross-sectional evaluation. Tumor specimens were collected following breast surgery, stained with methylene blue, and imaged. Wide-field fluorescence polarization images were excited at 640 nm and registered between 660 and 750 nm. Standard and PS OCT images were acquired using a commercial 1,310-nm swept-source system. The imaging results were validated against histopathology. Statistically significant higher fluorescence polarization of cancer as compared with both normal and fibrocystic tumor tissue was measured in all the samples. Fluorescence polarization delineated lateral breast cancer margins with contrast superior to that provided by OCT. However, OCT complemented fluorescence polarization imaging by facilitating cross-sectional inspection of tissue. PS OCT yielded higher contrast between cancer and connective tissue, as compared with standard OCT. Combined PS OCT and fluorescence polarization imaging shows promise for intraoperative delineation of breast cancer. Cancer Res; 74(17); 4685-93. Ó2014 AACR.

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Section: Contrast Agentmentioning

confidence: 99%

Polarization-Sensitive Multimodal Imaging for Detecting Breast Cancer

et al. 2014

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“…18,19 MB has also been shown to closely mimic H&E staining pattern of histopathology in vivo 24 and ex vivo. 20,25,26 For this study, commercially available methylene blue (MB 1% injection, USP, American Regent Laboratories, Inc., Shirley, NY) was diluted to a concentration of 0.05 mg∕ml with Dulbecco phosphate buffered saline solution (DPBS 1×, pH 7.4, Mediatech, Mannassas, Virginia).…”

Section: Contrast Agentsmentioning

confidence: 99%

“…The depth of polarization imaging is defined by the inverse of the reduced scattering coefficient of breast tissue. 20 Using optical properties of bloodless breast tissue reported in the literature, 30 we have estimated the imaging depth of polarization macro-imaging to be approximately between 320 μm-620 μm in the visible spectral range.…”

Section: Selection Of Imaging Modesmentioning

confidence: 99%

“…Wide-field polarization imaging allows for a rapid macroscopic overview of the entire surface area of the tissue specimen, 18,19 whereas confocal microscopy enables high-resolution imaging with a small field of view. 20 Two major types of breast cancers, including ductal and lobular carcinomas, were examined. Wide-field and high-resolution images of fresh thick breast cancer excisions stained with methylene blue (MB) were acquired.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal optical imaging for detecting breast cancer

et al. 2012

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Abstract. The goal of the study was to evaluate wide-field and high-resolution multimodal optical imaging, including polarization, reflectance, and fluorescence for the intraoperative detection of breast cancer. Lumpectomy specimens were stained with 0.05 mg∕ml aqueous solution of methylene blue (MB) and imaged. Wide-field reflectance images were acquired between 390 and 750 nm. Wide-field fluorescence images were excited at 640 nm and registered between 660 and 750 nm. High resolution confocal reflectance and fluorescence images were excited at 642 nm. Confocal fluorescence images were acquired between 670 nm and 710 nm. After imaging, the specimens were processed for hematoxylin and eosin (H&E) histopathology. Histological slides were compared with wide-field and high-resolution optical images to evaluate correlation of tumor boundaries and cellular morphology, respectively. Fluorescence polarization imaging identified the location, size, and shape of the tumor in all the cases investigated. Averaged fluorescence polarization values of tumor were higher as compared to normal tissue. Statistical analysis confirmed the significance of these differences. Fluorescence confocal imaging enabled cellularlevel resolution. Evaluation and statistical analysis of MB fluorescence polarization values registered from single tumor and normal cells demonstrated higher fluorescence polarization from cancer. Wide-field high-resolution fluorescence and fluorescence polarization imaging shows promise for intraoperative delineation of breast cancers.

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“…Fundamental theoretical and experimental discoveries and observations [13][14][15] have contributed to the advent and development of dermatological and cosmetic therapies [16]. A number of optical techniques have been suggested for noninvasive detection and treatment guidance of different pathological conditions of the skin and for the noninvasive assessment of skin treatment efficacy [9,11,17,18]. The transfer of optical radiation into the skin is determined by the absorption and scattering properties of its three functional layers: epidermis, dermis, and hypodermis.…”

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confidence: 99%