Multimodal optical imaging for detecting breast cancer

Patel, Rakesh; Khan, Ashraf; Wirth, Dennis J.; Kamionek, Michal; Kandil, Dina; Quinlan, Robert M.; Yaroslavsky, Anna N.

doi:10.1117/1.jbo.17.6.066008

Cited by 34 publications

(25 citation statements)

References 32 publications

(39 reference statements)

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“…The mean fluorescence polarization of normal, fibrocystic, and cancerous areas measured over all samples in this study was found to be 0.03 AE 0.01, 0.02 AE 0.01, and 0.06 AE 0.02, respectively. The fluorescence polarization values found for normal and cancer tissue in this study are in good accordance with previously measured values from our earlier study (15). Higher fluorescence polarization of cancer with respect to benign tissue was measured in all the samples.…”

Section: Resultssupporting

confidence: 81%

“…For correlating optical and histologic images and for evaluating cancer contrast in the optical images, the tumor and normal areas were outlined in the optical images of each specimen, exactly as they present in histopathology. This procedure was described in detail elsewhere (15,16,35,36). In short, cancerous and normal regions were outlined by a pathologist in digitized histopathology slides.…”

Section: Evaluation Of Tumor Contrast In Optical Imagesmentioning

confidence: 99%

“…[15][16][17][18][19] and optical coherence tomography (OCT; refs. [20][21][22] can potentially perform rapid and accurate evaluation of the entire surgical margin intraoperatively.…”

Section: Introductionmentioning

confidence: 99%

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Polarization-Sensitive Multimodal Imaging for Detecting Breast Cancer

et al. 2014

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Intraoperative delineation of breast cancer is a significant problem in surgical oncology. A reliable method for demarcation of malignant breast tissue during surgery would reduce the re-excision rate due to positive margins. We present a novel method of identifying breast cancer margins using combined dye-enhanced wide-field fluorescence polarization imaging for en face cancer margins and polarization-sensitive (PS) optical coherence tomography (OCT) for cross-sectional evaluation. Tumor specimens were collected following breast surgery, stained with methylene blue, and imaged. Wide-field fluorescence polarization images were excited at 640 nm and registered between 660 and 750 nm. Standard and PS OCT images were acquired using a commercial 1,310-nm swept-source system. The imaging results were validated against histopathology. Statistically significant higher fluorescence polarization of cancer as compared with both normal and fibrocystic tumor tissue was measured in all the samples. Fluorescence polarization delineated lateral breast cancer margins with contrast superior to that provided by OCT. However, OCT complemented fluorescence polarization imaging by facilitating cross-sectional inspection of tissue. PS OCT yielded higher contrast between cancer and connective tissue, as compared with standard OCT. Combined PS OCT and fluorescence polarization imaging shows promise for intraoperative delineation of breast cancer. Cancer Res; 74(17); 4685-93. Ó2014 AACR.

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Section: Resultssupporting

confidence: 81%

Section: Evaluation Of Tumor Contrast In Optical Imagesmentioning

confidence: 99%

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Polarization-Sensitive Multimodal Imaging for Detecting Breast Cancer

et al. 2014

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen-or fixed-section processing. Initial studies have described the major findings of invasive breast cancers using fluorescence confocal microscopy.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination

et al. 2017

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Abstract. Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen-or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750 × 750 μm 2 . We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2 × 2 cm 2 of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant.

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“…These include methodology based on Raman spectroscopy (10,11), scanning in situ spectroscopy and mapping of whole specimens (12), combined diffuse reflectance spectroscopy and intrinsic autofluorescence (10,13), or multimodal optical imaging (14). These studies have been exploratory and have examined and mapped tissue distribution in whole excised specimens ex vivo.…”

Section: Introductionmentioning

confidence: 99%

Cancer Detection in Human Tissue Samples Using a Fiber-Tip pH Probe

et al. 2016

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Intraoperative detection of tumorous tissue is an important unresolved issue for cancer surgery. Difficulty in differentiating between tissue types commonly results in the requirement for additional surgeries to excise unremoved cancer tissue or alternatively in the removal of excess amounts of healthy tissue. Although pathologic methods exist to determine tissue type during surgery, these methods can compromise postoperative pathology, have a lag of minutes to hours before the surgeon receives the results of the tissue analysis, and are restricted to excised tissue. In this work, we report the development of an optical fiber probe that could potentially find use as an aid for margin detection during surgery. A fluorophore-doped polymer coating is deposited on the tip of an optical fiber, which can then be used to record the pH by monitoring the emission spectra from this dye. By measuring the tissue pH and comparing with the values from regular tissue, the tissue type can be determined quickly and accurately. The use of a novel lift-and-measure technique allows for these measurements to be performed without influence from the inherent autofluorescence that commonly affects fluorescence-based measurements on biological samples. The probe developed here shows strong potential for use during surgery, as the probe design can be readily adapted to a low-cost portable configuration, which could find use in the operating theater. Use of this probe in surgery either on excised or in vivo tissue has the potential to improve success rates for complete removal of cancers. Cancer Res; 76(23); 6795–801. ©2016 AACR.

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Multimodal optical imaging for detecting breast cancer

Cited by 34 publications

References 32 publications

Polarization-Sensitive Multimodal Imaging for Detecting Breast Cancer

Polarization-Sensitive Multimodal Imaging for Detecting Breast Cancer

Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination

Cancer Detection in Human Tissue Samples Using a Fiber-Tip pH Probe

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