Multimaterial, heterogeneous, and multicellular three-dimensional bioprinting

Maria, Carmelo De; Vozzi, Giovanni; Moroni, Lorenzo

doi:10.1557/mrs.2017.165

Cited by 23 publications

(22 citation statements)

References 72 publications

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“…This permitted an increase in mechanics with light exposure and permitted patterning of dyes with light exposure through a mask. Printing of these networks may permit the fabrication of complex biological scaffolds with controlled heterogeneity in mechanics and biomolecule presentation across multiple length scales . This can have major advantages to the field of tissue engineering, where we are currently limited by the biological complexity of engineered scaffolds.…”

Section: Resultsmentioning

confidence: 99%

Three‐dimensional extrusion bioprinting of single‐ and double‐network hydrogels containing dynamic covalent crosslinks

Wang

Highley

Yeh

et al. 2018

J Biomedical Materials Res

240

274

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The fabrication of three-dimensional (3D) scaffolds is indispensable to tissue engineering and 3D printing is emerging as an important approach towards this. Hydrogels are often used as inks in extrusion-based 3D printing, including with encapsulated cells; however, numerous challenging requirements exist, including appropriate viscosity, the ability to stabilize after extrusion, and cytocompatibility. Here, we present a shear-thinning and self-healing hydrogel crosslinked through dynamic covalent chemistry for 3D bioprinting. Specifically, hyaluronic acid was modified with either hydrazide or aldehyde groups and mixed to form hydrogels containing a dynamic hydrazone bond. Due to their shear-thinning and self-healing properties, the hydrogels could be extruded for 3D printing of structures with high shape fidelity, stability to relaxation, and cytocompatibility with encapsulated fibroblasts (>80% viability). Forces for extrusion and filament sizes were dependent on parameters such as material concentration and needle gauge. To increase scaffold functionality, a second photocrosslinkable interpenetrating network was included that was used for orthogonal photostiffening and photopatterning through a thiol-ene reaction. Photostiffening increased the scaffold's modulus (∼300%) while significantly decreasing erosion (∼70%), whereas photopatterning allowed for spatial modification of scaffolds with dyes. Overall, this work introduces a simple approach to both fabricate and modify 3D printed scaffolds. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 865-875, 2018.

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Section: Resultsmentioning

confidence: 99%

Three‐dimensional extrusion bioprinting of single‐ and double‐network hydrogels containing dynamic covalent crosslinks

Wang

Highley

Yeh

et al. 2018

J Biomedical Materials Res

240

274

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show abstract

“…In their article, De Maria et al 18 consider how to implement more advanced bioprinting systems that use multiple extruders to fabricate multimaterial, heterogeneous, and multicellular 3D structures. This is a critical capability as researchers seek to use 3D bioprinting to recreate the complexity of native tissues and organs.…”

Section: In This Issue: Materials-focused Challenges and Applicationsmentioning

confidence: 99%

Progress in three-dimensional bioprinting

Feinberg

Miller

2017

MRS Bull.

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“…fibroblasts, keratinocytes, chondrocytes, stem cells) [7]. Advances in the field of additive manufacturing allowed to create multi-material and multi-cellular constructs using multi-head printing systems [8,9]. However, each printing head usually operates with a single bioink.…”

Section: Introductionmentioning

confidence: 99%

A novel printing channel design for multi-material extrusion additive manufacturing

Urhal

2020

MATEC Web Conf.

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Additive manufacturing has a great potential in terms of its capability to produce components with complex geometries and to make multi-material and composite products by combining different materials in a single manufacturing platform. Current trends for the multi-material extrusion additive manufacturing process are categorized by multi-nozzle systems and multi-material inlet systems. In the case of multiple nozzle system, materials are deposited from different nozzles in sequence. On the other hand, in the case of multi-material inlet system, different materials are sent into a mixing tube and deposited as a mixture of materials. In this case, functionally graded parts can be fabricated by changing the volume fraction of two or more materials. Hence, the fabrication of parts with a continuous material supply by varying ratios for the extrusion technologies requires the development of printing heads with suitable printing channels, capable of varying the mixing ratio of different materials. To evaluate the effect of different printing channel designs on the material’s flow pattern and the functionally graded material printability, this paper presents a three-dimensional transient computational fluid dynamics (CFD) simulation of the two miscible liquid-liquid system in a printing channel. Different geometries and materials are considered

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Multimaterial, heterogeneous, and multicellular three-dimensional bioprinting

Cited by 23 publications

References 72 publications

Three‐dimensional extrusion bioprinting of single‐ and double‐network hydrogels containing dynamic covalent crosslinks

Three‐dimensional extrusion bioprinting of single‐ and double‐network hydrogels containing dynamic covalent crosslinks

Progress in three-dimensional bioprinting

A novel printing channel design for multi-material extrusion additive manufacturing

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