Progress in three-dimensional bioprinting

Feinberg, Adam W.; Miller, Jordan S.

doi:10.1557/mrs.2017.166

Cited by 37 publications

(26 citation statements)

References 33 publications

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“…Syringe-pump-based 3D printers can be generically broken down into two categories: bioprinters and paste/clay extruders. Bioprinters are designed for high-precision printing of biopolymers and cells, and usually feature low-volume syringes and small-diameter nozzles (< 250 µm) to print small objects [ 1 – 3 ]. Conversely, paste extruders are designed for high-capacity, often featuring large material reservoirs and large-diameter nozzles (up to 4 mm).…”

Section: Hardware In Contextmentioning

confidence: 99%

Large volume syringe pump extruder for desktop 3D printers

2018

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Syringe pump extruders are required for a wide range of 3D printing applications, including bioprinting, embedded printing, and food printing. However, the mass of the syringe becomes a major challenge for most printing platforms, requiring compromises in speed, resolution and/or volume. To address these issues, we have designed a syringe pump large volume extruder (LVE) that is compatible with low-cost, open source 3D printers, and herein demonstrate its performance on a PrintrBot Simple Metal. Key aspects of the LVE include: (1) it is open source and compatible with open source hardware and software, making it inexpensive and widely accessible to the 3D printing community, (2) it utilizes a standard 60 mL syringe as its ink reservoir, effectively increasing print volume of the average bioprinter, (3) it is capable of retraction and high speed movements, and (4) it can print fluids using nozzle diameters as small as 100 µm, enabling the printing of complex shapes/objects when used in conjunction with the freeform reversible embedding of suspended hydrogels (FRESH) 3D printing method. Printing performance of the LVE is demonstrated by utilizing alginate as a model biomaterial ink to fabricate parametric CAD models and standard calibration objects.

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Section: Hardware In Contextmentioning

confidence: 99%

Large volume syringe pump extruder for desktop 3D printers

2018

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“…Extrusion allows for printing of highly viscous, cell-dense bioinks; however, cells experience high shear stresses by moving through the tip. Finally, laser techniques include laser-assisted bioprinting based on the laser-induced forward-transfer (LIFT) where a pulsed laser forms a bubble that transfers the ink to an absorbing layer below [32] and stereolithography (SLA) that patterns photosensitive solutions by light exposure [33]. SLA is sometimes considered a separate technique because of its need for photopolymerization [34].…”

Section: 3d Bioprinting Techniquesmentioning

confidence: 99%

iPSC Bioprinting: Where are We at?

2019

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Here, we present a concise review of current 3D bioprinting technologies applied to induced pluripotent stem cells (iPSC). iPSC have recently received a great deal of attention from the scientific and clinical communities for their unique properties, which include abundant adult cell sources, ability to indefinitely self-renew and differentiate into any tissue of the body. Bioprinting of iPSC and iPSC derived cells combined with natural or synthetic biomaterials to fabricate tissue mimicked constructs, has emerged as a technology that might revolutionize regenerative medicine and patient-specific treatment. This review covers the advantages and disadvantages of bioprinting techniques, influence of bioprinting parameters and printing condition on cell viability, and commonly used iPSC sources, and bioinks. A clear distinction is made for bioprinting techniques used for iPSC at their undifferentiated stage or when used as adult stem cells or terminally differentiated cells. This review presents state of the art data obtained from major searching engines, including Pubmed/MEDLINE, Google Scholar, and Scopus, concerning iPSC generation, undifferentiated iPSC, iPSC bioprinting, bioprinting techniques, cartilage, bone, heart, neural tissue, skin, and hepatic tissue cells derived from iPSC.

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“…Techniques that are amenable to creating custom-made, reproducible, intricate 3D designs using cytocompatible materials would be ideal for tissue engineering. 3D printing, or bioprinting when cells are printed, has recently emerged as a potential source for bioengineering tissues or supporting structures [92][93][94][95][96][97][98]. 3D printing has been applied clinically for the treatment of tracheobronchomalacia [99] and tracheal collapse with 3D printed patient-specific tracheal splints [100].…”

Section: Artificial Lung Scaffoldsmentioning

confidence: 99%

How to build a lung: latest advances and emerging themes in lung bioengineering

et al. 2018

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Chronic respiratory diseases remain a major cause of morbidity and mortality worldwide. The only option at end-stage disease is lung transplantation, but there are not enough donor lungs to meet clinical demand. Alternative options to increase tissue availability for lung transplantation are urgently required to close the gap on this unmet clinical need. A growing number of tissue engineering approaches are exploring the potential to generate lung tissue for transplantation. Both biologically derived and manufactured scaffolds seeded with cells and grown have been explored in pre-clinical studies, with the eventual goal of generating functional pulmonary tissue for transplantation. Recently, there have been significant efforts to scale-up cell culture methods to generate adequate cell numbers for human-scale bioengineering approaches. Concomitantly, there have been exciting efforts in designing bioreactors that allow for appropriate cell seeding and development of functional lung tissue over time. This review aims to present the current state-of-the-art progress for each of these areas and to discuss promising new ideas within the field of lung bioengineering.

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Progress in three-dimensional bioprinting

Abstract: Abstract

Cited by 37 publications

References 33 publications

Large volume syringe pump extruder for desktop 3D printers

Large volume syringe pump extruder for desktop 3D printers

iPSC Bioprinting: Where are We at?

How to build a lung: latest advances and emerging themes in lung bioengineering

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