Multilocus Sequencing ofCorynebacterium pseudotuberculosisBiotype Ovis Strains

Abstract

Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). The in silico analysis of the 16 frequently studied housekeeping genes showed that C. pseudotuberculosis strains could be readily distinguished from C. diphtheriae and C. ulcerans strains; however, sequences of the same genes in the two biotypes of the C. pseudotuberculosis were highly similar; the heterogeneity values were low. Genes dnaK, infB, groEL1, and leuA showed marked genetic variation within C. pseudotuberculosis, and strains of the two biotypes of C. pseudotuberculosis could be differentiated. Analysis of the individual genes showed a fairly conservative nature of C. pseudotuberculosis biotype ovis strains. The greatest genetic differentiation was seen in the dnaK and infB genes and concatenations of these two genes were very useful in the genetic separation of the studied strains.

Molecular Characterization of Corynebacterium pseudotuberculosis Isolated over a 15-Year Period in Switzerland

“…Gene-by-gene approaches, such as MLST and MLSA, are one of the most important tools in modern molecular epidemiology; however, they have not been widely adopted for C. pseudotuberculosis , in contrast to C. ulcerans and C. diphtheriae [ 49 , 50 ]. Sellyei et al (2017) established the first MLSA scheme for C. pseudotuberculosis and reported great genetic variability [ 48 ]. For the present MLSA, four genes ( groEL1 , infB , leuA , dnaK ) of the before-mentioned study were retained and one new locus ( aspT ) was added to improve the discriminatory capacity of the analysis.…”

“…Sellyei et al (2017) analyzed thirteen C. pseudotuberculosis biovar Ovis field strains from Hungary and nine strains from the NCBI database; one DSM reference strain (DSM 7180) was also included in their analysis. They distinguished 23 STs among the biovar Ovis isolates [ 48 ]. However, it must be considered that the strains analyzed in the mentioned study originated from diverse geographical areas and various hosts, including buffalo, llama, antelope and human.…”

“…A total of five genes including the heat-shock protein ( groEL1 ), the translation initiation factor IF2 ( infB ), the 2-isopropylmalate synthase ( leuA ), the chaperone Hsp70 ( dnaK ) and an aspartate aminotransferase ( aspT ) were chosen for MLSA ( Table 1 ) [ 48 ]. The location of the five genes in the genome of the DSM 20,689 reference strain C. pseudotuberculosis biovar Ovis (Acc.…”

“…MLSA is a more flexible alternative for comparing bacteria; here, the actual DNA sequences are used in the downstream analyses. To date, limited data are available on the performance and discriminatory power of MLST and MLSA in C. pseudotuberculosis [ 48 ], although MLST has been proven to be useful for the characterization of C. ulcerans [ 49 ] and C. diphtheriae [ 50 ], which are both closely related to C. pseudotuberculosis . Since a reliable and reproducible molecular typing method with good discriminatory power is needed for C. pseudotuberculosis , the performance of a sequence-based molecular typing method should be evaluated.…”

Effectiveness of Disinfectants on Environmental Multidrug Resistance Contaminants Causing Skin Abscess in Farm Animals

“…Corynebacterium pseudotuberculosis is categorized by two biovars (Sellyei, et al, 2017) the biovar Ovis, which at most affects goats and sheep, causes superficial and visceral abscesses, mainly affects horses, causes ulcerative lymphangitis of the abdominal abscesses in the chest and abdomen, distal extremities and hematomas. The presence of these two types of biovar has been assured by bimolecular techniques (Connor et al, 2007).…”