Multilocus phylogenetic analyses, pullulan production and xylanase activity of tropical isolates of Aureobasidium pullulans

Aureobasidium thailandense sp. nov. isolated from leaves and wooden surfaces

“…nov. demonstrated an approx. 500 bp insert in the 39 end of the 18S rRNA gene from isolates NRRL 58539 T and NRRL 58543, but sequencing of numerous A. pullulans isolates revealed no introns (Manitchotpisit et al, 2009). In a BLAST search of the GenBank database, this rRNA insert segment demonstrated 100 % similarity to the comparable region from Neoscytalidium dimidiatum over 68 % of the segment length and probably represents a group I intron in the nuclear SSU-rRNA gene (Bhattacharya et al, 2005).…”

“…It has been isolated from the phylloplanes of diverse plants, maize silks, brackish water, mangrove swamps, glacial ice in Norway and wooden or painted structures (Arfi et al, 2012;Manitchotpisit et al, 2009;Santo et al, 2012;Zalar et al, 2008). Aureobasidium proteae was reported to cause chronic fungal meningitis (Kutleša et al, 2012) as well as a leaf spot disease of Proteaceae (Crous et al, 2011).…”

“…Aureobasidium proteae was reported to cause chronic fungal meningitis (Kutleša et al, 2012) as well as a leaf spot disease of Proteaceae (Crous et al, 2011). Some isolates of A. pullulans produce semiochemicals (Davis et al, 2012) that influence wasp behaviour in nature and that may be involved in dispersal of members of the genus Aureobasidium, while other isolates produce large amounts of the extracellular polysaccharide (EPS) pullulan (Manitchotpisit et al, 2009), a polysaccharide with many valuable industrial and medical uses (Leathers, 2002;Cheng et al, 2011). Still other isolates biosynthesize products that have commercial value such as laccase (Rich et al, 2011), xylanase (Manitchotpisit et al, 2009) or poly b-L-malic acid (Manitchotpisit et al, 2012).…”

“…The taxonomy of the genus Aureobasidium as viewed by different authors has consisted of its division into phenotypic varieties of A. pullulans (Hermanides-Nijhof, 1977;Zalar et al, 2008), descriptions of monophyletic clades in A. pullulans without specific Linnaean ranking (Manitchotpisit et al, 2009) or descriptions of distinct species (Crous et al, 2011). The broad clade containing the genus Aureobasidium (Schoch et al, 2006;Bills et al, 2012) includes other genera, such as Kabatiella and Selenophoma that express Aureobasidium and other anamorphic states under different growth conditions (synanamorphy).…”

“…The broad clade containing the genus Aureobasidium (Schoch et al, 2006;Bills et al, 2012) includes other genera, such as Kabatiella and Selenophoma that express Aureobasidium and other anamorphic states under different growth conditions (synanamorphy). Manitchotpisit et al (2009) isolated two putative Aureobasidium pullulans strains whose phylogenetic position was not fully resolved. These two isolates contain an 18S rRNA intron not found in other A. pullulans isolates and produce an uncharacterized EPS.…”