Abstract:Aureobasidium thailandense sp. nov. is described from cultures of material collected on leaves and wooden surfaces in Thailand and the type isolate is NRRL 58539T. Phylogenetically it is distinct from other species of the genus Aureobasidium. Phenotypically it is distinguished by its cardinal growth temperatures, salt tolerance and production of reddish brown hyphal pigmentation in PDA cultures, but micro-morphologically it is not clearly distinguishable from Aureobasidium pullulans. Unlike A. pullulans, A. th… Show more
“…The most recently described species of Aureobasidium are A. leucospermi Crous [47], A. proteae (Joanne E. Taylor & Crous) Joanne E. Taylor & Crous 2011 [47], and A. thailandense S.W. Peterson, Manitchotpisit & Leathers [48]. Figure 1
The
Aureobasidium pullulans
varieties.…”
BackgroundAureobasidium pullulans is a black-yeast-like fungus used for production of the polysaccharide pullulan and the antimycotic aureobasidin A, and as a biocontrol agent in agriculture. It can cause opportunistic human infections, and it inhabits various extreme environments. To promote the understanding of these traits, we performed de-novo genome sequencing of the four varieties of A. pullulans.ResultsThe 25.43-29.62 Mb genomes of these four varieties of A. pullulans encode between 10266 and 11866 predicted proteins. Their genomes encode most of the enzyme families involved in degradation of plant material and many sugar transporters, and they have genes possibly associated with degradation of plastic and aromatic compounds. Proteins believed to be involved in the synthesis of pullulan and siderophores, but not of aureobasidin A, are predicted. Putative stress-tolerance genes include several aquaporins and aquaglyceroporins, large numbers of alkali-metal cation transporters, genes for the synthesis of compatible solutes and melanin, all of the components of the high-osmolarity glycerol pathway, and bacteriorhodopsin-like proteins. All of these genomes contain a homothallic mating-type locus.ConclusionsThe differences between these four varieties of A. pullulans are large enough to justify their redefinition as separate species: A. pullulans, A. melanogenum, A. subglaciale and A. namibiae. The redundancy observed in several gene families can be linked to the nutritional versatility of these species and their particular stress tolerance. The availability of the genome sequences of the four Aureobasidium species should improve their biotechnological exploitation and promote our understanding of their stress-tolerance mechanisms, diverse lifestyles, and pathogenic potential.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-549) contains supplementary material, which is available to authorized users.
“…The most recently described species of Aureobasidium are A. leucospermi Crous [47], A. proteae (Joanne E. Taylor & Crous) Joanne E. Taylor & Crous 2011 [47], and A. thailandense S.W. Peterson, Manitchotpisit & Leathers [48]. Figure 1
The
Aureobasidium pullulans
varieties.…”
BackgroundAureobasidium pullulans is a black-yeast-like fungus used for production of the polysaccharide pullulan and the antimycotic aureobasidin A, and as a biocontrol agent in agriculture. It can cause opportunistic human infections, and it inhabits various extreme environments. To promote the understanding of these traits, we performed de-novo genome sequencing of the four varieties of A. pullulans.ResultsThe 25.43-29.62 Mb genomes of these four varieties of A. pullulans encode between 10266 and 11866 predicted proteins. Their genomes encode most of the enzyme families involved in degradation of plant material and many sugar transporters, and they have genes possibly associated with degradation of plastic and aromatic compounds. Proteins believed to be involved in the synthesis of pullulan and siderophores, but not of aureobasidin A, are predicted. Putative stress-tolerance genes include several aquaporins and aquaglyceroporins, large numbers of alkali-metal cation transporters, genes for the synthesis of compatible solutes and melanin, all of the components of the high-osmolarity glycerol pathway, and bacteriorhodopsin-like proteins. All of these genomes contain a homothallic mating-type locus.ConclusionsThe differences between these four varieties of A. pullulans are large enough to justify their redefinition as separate species: A. pullulans, A. melanogenum, A. subglaciale and A. namibiae. The redundancy observed in several gene families can be linked to the nutritional versatility of these species and their particular stress tolerance. The availability of the genome sequences of the four Aureobasidium species should improve their biotechnological exploitation and promote our understanding of their stress-tolerance mechanisms, diverse lifestyles, and pathogenic potential.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-549) contains supplementary material, which is available to authorized users.
“…In some cases, phenotypic identification does not linier with genotype approach (Kurtzman & Suzuki, 2010). As well found in genera of Aureobasidium (Zalar et al, 2008;Peterson et al, 2013). For example, study reported that A. pullulans strains which delineated by phylogenetic tree showed they have distinct clade with each color formation and organic material production such as xylanase, feruloyl esterase, and pullulan.…”
Section: Identification and Phylogenetic Analysismentioning
The identification of yeasts or yeasts-like fungi and verify their diversity are principal aspect for bioindustry and ecosystem sustainability. Taxonomic approach provides identification tool to ensure the taxonomic position of yeasts and yeasts-like fungi which definitely set to utilization concerns. The aim of this study is to understanding the taxonomic position of yeasts and yeasts-like fungi from the distinctive of its sequences relationship. Yeasts and yeasts-like fungi strains were isolated through various culture dependent methods from natural resources samples of Karimun Besar Island, Province of Riau Islands, Indonesia. The identification process was performed through amplifying the accurate DNA-based in D1/D2 region of large subunit (26S) ribosomal DNA. As the result, a total of 85 isolates of yeasts and yeasts-like fungi were obtained with 16 closest related taxa through phylogenetic tree construction. Ascomycetous was the predominating group representing 91% of the total isolates sequences followed by Basidiomycetous (8%) and Zygomycetous (1%). The black yeasts (yeasts-like) known as Aureobasidium melanogenum was predominant species with represent to 54% of total isolates and present in particular habitat. Taxonomically, there are six isolates are represent to be novel taxa candidates which pretend to enhance genetic resources of yeasts and yeasts-like fungi especially from Indonesia. In addition, this information provides specific technique to reach specific yeasts or yeasts-like fungi species in nature by managing the sample collection and culture methods.
“…The ascomycete genus Aureobasidium is a member of the family Aureobasidiaceae within the class of the Dothideomycetes (Thambugala et al 2014 ; Wijayawardene et al 2014 ). Kabatiella is closely related to Aureobasidium based on morphology and phylogeny (Zalar et al 2008 ; Bills et al 2012 ; Crous et al 2011 ; Peterson et al 2013 ; Thambugala et al 2014 ) and some of these Kabatiella species may belong to Aureobasidium (Peterson et al 2013 ; Thambugala et al 2014 ). In addition future studies may result in the transfer of the species Selenophoma mahoniae and Columnosphaeria ( Discosphaerina ) fagi into Aureobasidium (Yurlova et al 1999 ; Peterson et al 2013 ; Thambugala et al 2014 ).…”
Section: Introductionmentioning
confidence: 99%
“…Kabatiella is closely related to Aureobasidium based on morphology and phylogeny (Zalar et al 2008 ; Bills et al 2012 ; Crous et al 2011 ; Peterson et al 2013 ; Thambugala et al 2014 ) and some of these Kabatiella species may belong to Aureobasidium (Peterson et al 2013 ; Thambugala et al 2014 ). In addition future studies may result in the transfer of the species Selenophoma mahoniae and Columnosphaeria ( Discosphaerina ) fagi into Aureobasidium (Yurlova et al 1999 ; Peterson et al 2013 ; Thambugala et al 2014 ). A well-known Aureobasidium species is Aureobasidium pullulans (Zalar et al 2008 ; Gostinčar et al 2014 ).…”
The genus Aureobasidium, which is known as a wood staining mould, has been detected on oil treated woods in the specific stain formation called biofinish. This biofinish is used to develop a new protective, self-healing and decorative biotreatment for wood. In order to understand and control biofinish formation on oil treated wood, the occurrence of different Aureobasidium species on various wood surfaces was studied. Phenotypic variability within Aureobasidium strains presented limitations of morphological identification of Aureobasidium species. PCR amplification and Sanger sequencing of ITS and RPB2 were used to identify the culturable Aureobasidium species composition in mould stained wood surfaces with and without a biofinish. The analysed isolates showed that several Aureobasidium species were present and that Aureobasidium melanogenum was predominantly detected, regardless of the presence of a biofinish and the type of substrate. A.melanogenum was detected on wood samples exposed in the Netherlands, Cameroon, South Africa, Australia and Norway. ITS-specific PCR amplification, cloning and sequencing of DNA extracted from biofinish samples confirmed results of the culturing based method: A. melanogenum is predominant within the Aureobasidium population of biofinishes on pine sapwood treated with raw linseed oil and the outdoor placement in the Netherlands.Electronic supplementary materialThe online version of this article (doi:10.1007/s10482-016-0668-7) contains supplementary material, which is available to authorized users.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
