Multilevel heuristic LED regime for stimulating lipid and bioproducts biosynthesis in Haematococcus pluvialis under mixotrophic conditions

Pang, Na; Xiao, Fu; Martinez-Fernandez, Jose S.; Chen, Shulin

doi:10.1016/j.biortech.2019.121525

Cited by 27 publications

(18 citation statements)

References 39 publications

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“…Various carbon sources have been studied for mixotrophic or heterotrophic cultivation of H. pluvialis, including acetate, glucose, glycerol, rice straw hydrolyzates, and organic-rich wastewaters (Leite et al, 2015;Gupta et al, 2019). H. pluvialis can also utilize exogenous organic carbon substrates (Kobayashi et al, 1992), and sodium acetate (NaAC) was considered to significantly accelerate cell growth or astaxanthin accumulation (Kobayashi et al, 1993;Orosa et al, 2001;Tolga et al, 2010;Pang et al, 2019;Zhang et al, 2019;Cong et al, 2020). In H. pluvialis, exogenous NaAC increases respiratory rates and suppresses photosynthetic activity in the cells (Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

Song

Huang

et al. 2021

Front. Plant Sci.

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To elucidate the mechanism underlying increased fatty acid and astaxanthin accumulation in Haematococcus pluvialis, transcriptome analysis was performed to gain insights into the multiple defensive systems elicited by salicylic acid combined with sodium acetate (SAHS) stresses with a time course. Totally, 112,886 unigenes and 61,323 non-repeat genes were identified, and genes involved in carbon metabolism, primary and secondary metabolism, and immune system responses were identified. The results revealed that SA and NaAC provide both energy and precursors to improve cell growth of H. pluvialis and enhance carbon assimilation, astaxanthin, and fatty acids production in this microalga with an effective mechanism. Interestingly, SA was considered to play an important role in lowering transcriptional activity of the fatty acid and astaxanthin biosynthesis genes through self-protection metabolism in H. pluvialis, leading to its adaption to HS stress and finally avoiding massive cell death. Moreover, positive correlations between 15 key genes involved in astaxanthin and fatty acid biosynthesis pathways were found, revealing cooperative relation between these pathways at the transcription level. These results not only enriched our knowledge of the astaxanthin accumulation mechanism in H. pluvialis but also provided a new view on increasing astaxanthin production in H. pluvialis by a moderate and sustainable way in the future.

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Section: Introductionmentioning

confidence: 99%

Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

Song

Huang

et al. 2021

Front. Plant Sci.

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“…Since all microalgae contain chlorophyll a , red and blue light is the major wavelength that efficiently supports photosynthesis as illustrated in Figure 1. Likewise, red LED was reported to accelerate cell growth (Ma et al., 2018; Pang et al., 2019). This is because red light can provide the energy required by chlorophyll a or b to promote the electron flux and further stimulate the light‐harvesting antenna complex (Glemser et al., 2016).…”

Section: Biomassmentioning

confidence: 99%

“…For instance, the microalgae Haematococcus pluvialis requires certain stress to trigger the change of stage from green to red. It was reported that exposure of H. pluvialis to a high light intensity of white‐red LEDs showed enhanced biomass and lipid content compared to white‐blue LEDs (Pang et al., 2019). The change in light intensity of white‐red LEDs was beneficial for the biosynthesis of saturated fatty acids, while white‐blue light was more favourable for astaxanthin accumulation (Pang et al., 2019).…”

Section: Lipidmentioning

confidence: 99%

“…It was reported that exposure of H. pluvialis to a high light intensity of white‐red LEDs showed enhanced biomass and lipid content compared to white‐blue LEDs (Pang et al., 2019). The change in light intensity of white‐red LEDs was beneficial for the biosynthesis of saturated fatty acids, while white‐blue light was more favourable for astaxanthin accumulation (Pang et al., 2019). On the other hand, Nannochloropsis oceanica CY2 exposed to blue LED had the highest EPA (5.57%) compared to red, yellow and white LEDs (Chen et al., 2013).…”

Section: Lipidmentioning

confidence: 99%

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Influence of light on biomass and lipid production in microalgae cultivation

Kwan

Banerjee

Shariff

et al. 2020

Aquaculture Research

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Microalgae have a diverse application in food, pharmaceutical, beauty, animal feed and bioenergy industry. The demand for high‐quality pure microalgae biomass has steered the large‐scale production of microalgae in the photobioreactor. The usage of light‐emitting diodes (LEDs) as a light source is gaining attention as to conventional fluorescent lamps due to the higher energy conversion ratio, lower heat dissipation and its flexible application in photobioreactors. Besides, the narrow spectrum of LEDs could efficiently promote specific compounds in microalgae. This article reviews the influence of using various LED spectra on the biomass and lipid composition of microalgae. In addition, suggestions on the use of different LEDs cultivating common microalgae species in aquaculture are provided.

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“…However, the cost of materials and energy consumption might be too high to compete with phototrophic culture using sunlight. Recently, a novel ASX production process based on a mixotrophic mode has been developed with a heuristic multilevel LED light regime and the highest content of 3.3% was achieved at the white-blue regime [141]. Polyol alcohols (glycerol and mannitol) have also been shown to be more efficient carbon sources than acetate for the efficient and cost-effective ASX production from Haematococcus [142].…”

Section: Closed Systemsmentioning

confidence: 99%

Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development

Smaoui

Barkallah

Hlima

et al. 2021

Foods

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In the last 20 years, xanthophylls from microalgae have gained increased scientific and industrial interests. This review highlights the essential issues that concern this class of high value compounds. Firstly, their chemical diversity as the producer microorganisms was detailed. Then, the use of conventional and innovative extraction techniques was discussed. Upgraded knowledge on the biosynthetic pathway of the main xanthophylls produced by photosynthetic microorganisms was reviewed in depth, providing new insightful ideas, clarifying the function of these active biomolecules. In addition, the recent advances in encapsulation techniques of astaxanthin and fucoxanthin, such as spray and freeze drying, gelation, emulsification and coacervation were updated. Providing information about these topics and their applications and advances could be a help to students and young researchers who are interested in chemical and metabolic engineering, chemistry and natural products communities to approach the complex thematic of xanthophylls.

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Multilevel heuristic LED regime for stimulating lipid and bioproducts biosynthesis in Haematococcus pluvialis under mixotrophic conditions

Cited by 27 publications

References 39 publications

Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

Influence of light on biomass and lipid production in microalgae cultivation

Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development

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