Multifunctionality of indocyanine green-loaded biodegradable nanoparticles for enhanced optical imaging and hyperthermia intervention of cancer

Abstract

Abstract. The aim of this study was to develop and characterize multifunctional biodegradable and biocompatible poly lactic-co-glycolic acid (PLGA) nanoparticles loaded with indocyanine green (ICG) as an optical-imaging contrast agent for cancer imaging and as a photothermal therapy agent for cancer treatment. PLGA-ICG nanoparticles (PIN) were synthesized with a particle diameter of 246 AE 11 nm, a polydispersity index of 0.10 AE 0.03, and ICG loading efficiency of 48.75 AE 5.48%. PIN were optically characterized with peak excitation and emission at 765 and 810 AE 5 nm, a fluorescence lifetime of 0.30 AE 0.01 ns, and peak absorbance at 780 nm. The cytocompatibility study of PIN showed 85% cell viability till 1-mg∕ml concentration of PIN. Successful cellular uptake of ligand conjugated PIN by prostate cancer cells (PC3) was also obtained. Both phantom-based and in vitro cell culture results demonstrated that PIN (1) have the great potential to induce local hyperthermia (i.e., temperature increase of 8 to 10°C) in tissue within 5 mm both in radius and in depth; (2) result in improved optical stability, excellent biocompatibility with healthy cells, and a great targeting capability; (3) have the ability to serve as an image contrast agent for deep-tissue imaging in diffuse optical tomography.

Polymeric nanoparticles for targeted radiosensitization of prostate cancer cells

“…The R11 conjugation onto the surface of our NPs also played a key role in active targeting and uptake of NU7441-loaded particles by prostate cancer cells. Our results concur with studies by Patel et al 29 which showed higher dose-dependent uptake of R11-conjugated PLGA NPs by PC3 cells when compared to NPs conjugated with folic acid and RGD-4C.…”

Review on near-infrared heptamethine cyanine dyes as theranostic agents for tumor imaging, targeting, and photodynamic therapy

“…36 Encapsulating NIRF dyes with polymers or inorganic materials to form a variety of nonmetallic nanoparticles also enhanced the photostability, biocompatibility, and fluorescence intensity of dyes with reduced self-aggregation to improve PDT efficacy. 42,[80][81][82] As a conventional NIR dye, ICG has limited applications as a specific imaging agent for cancer detection because of poor stability and rapid decomposition in polar solution. When ICG was attached to cationic polyethylene to form silicon nanoparticles, the degradation and fluorescence quenching of ICG was largely reduced.…”

Indocyanine Green Nanoparticles for Theranostic Applications

“…As an amphiphilic dye, ICG can be loaded into several materials including calcium phosphosilicate [13], poly(allylamine hydrochloride) assembled mesocapsules [14], PLGA [15], PLGA-PEG [16], PL-PEG [17], Pluronic F-127 [18], SiO 2 [19], ionic liquids [20], and so on. Among them, biodegradable-biocompatible, FDA approved PLGA carriers are one of the most common systems employed to improve ICG for theranostic applications [21].…”