Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection

Cicuéndez, Mónica; Doadrio, A.; Hernández, Ana; Portolés, María Teresa; Izquierdo‐Barba, Isabel; Vallet‐Regí, María

doi:10.1016/j.actbio.2017.11.009

Cited by 74 publications

(59 citation statements)

References 48 publications

(73 reference statements)

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“…Three‐dimensional scaffolds which allow bone generation alongside sustained release of antibiotic are under development (Cicuéndez et al . ) and it will be critical to their success that sufficient antibiotic can be delivered in order to suppress the development of SCVs.…”

Section: Resultsmentioning

confidence: 99%

Anin vitrobiofilm model ofStaphylococcus aureusinfection of bone

Sweeney

Lovering

Bowker

et al. 2019

Lett Appl Microbiol

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Significance and Impact of the Study: The majority of studies of antibiotic efficacy in the treatment of chronic osteomyelitis are carried out in animals. We developed an in vitro model of Staphylococcus aureus infection of bone to evaluate the ability of antibiotics to eradicate mature biofilms on surfaces analogous to necrotic bone. The results demonstrated the difficulties which occur in osteomyelitis treatment, with only very high concentrations of antibiotic able to penetrate the bone sufficiently to reduce bacterial survival whilst still failing to eradicate biofilms. This model could be of use in initial screening of novel compounds intended for use in the treatment of osteomyelitis. AbstractChronic osteomyelitis is difficult to treat, with biofilm growth and the diffusion barrier to antibiotics presented by bone contributory factors. The aim of this study was to develop and evaluate an in vitro model of osteomyelitis. A bioluminescent strain of Staphylococcus aureus was grown in bone blocks made from bovine femur. Light output was insufficient for detection of bacterial cells within bone by 24 h and viable counting of crushed bone blocks was used to determine bacterial survival. Challenge of 72 h biofilms with gentamicin and daptomycin for 24 h demonstrated that only concentrations of 10 times the clinical peak serum target levels (100 mg l À1 gentamicin and 1000 mg l À1 daptomycin) resulted in significant reductions in cell viability compared to controls. Once daily dosing over 7 days resulted in ≥3 log reductions in cell numbers by 48 h. Thereafter no significant reduction was achieved, although emergence of resistance was suppressed. Determination of antibiotic concentration in bone blocks over 7 days indicated that neither agent was able to consistently reach levels in bone of >10% of the original dose. The model was, therefore, able to demonstrate the challenges posed by biofilm growth on and within bone.

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Section: Resultsmentioning

confidence: 99%

Anin vitrobiofilm model ofStaphylococcus aureusinfection of bone

Sweeney

Lovering

Bowker

et al. 2019

Lett Appl Microbiol

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“…Vallet‐Regí and co‐workers developed multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection . The hierarchical meso‐macroporous 3D scaffolds consisted of a mesostructured SiO 2 –CaO–P 2 O 5 glass wall with embedded hydroxyapatite nanoparticles, whose mesopores were loaded with levofloxacin.…”

Section: Ms In Antibacterial Applicationsmentioning

confidence: 99%

Mesoporous Silica‐Based Materials with Bactericidal Properties

Bernardos

Piacenza

Sancenón

et al. 2019

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Bacterial infections are the main cause of chronic infections and even mortality. In fact, due to extensive use of antibiotics and, then, emergence of antibiotic resistance, treatment of such infections by conventional antibiotics has become a major concern worldwide. One of the promising strategies to treat infection diseases is the use of nanomaterials. Among them, mesoporous silica materials (MSMs) have attracted burgeoning attention due to high surface area, tunable pore/particle size, and easy surface functionalization. This review discusses how one can exploit capacities of MSMs to design and fabricate multifunctional/controllable drug delivery systems (DDSs) to combat bacterial infections. At first, the emergency of bacterial and biofilm resistance toward conventional antimicrobials is described and then how nanoparticles exert their toxic effects upon pathogenic cells is discussed. Next, the main aspects of MSMs (e.g., physicochemical properties, multifunctionality, and biosafety) which one should consider in the design of MSM‐based DDSs against bacterial infections are introduced. Finally, a comprehensive analysis of all the papers published dealing with the use of MSMs for delivery of antibacterial chemicals (antimicrobial agents functionalized/adsorbed on mesoporous silica (MS), MS‐loaded with antimicrobial agents, gated MS‐loaded with antimicrobial agents, MS with metal‐based nanoparticles, and MS‐loaded with metal ions) is provided.

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“…To this end, levofloxacin was incorporated into the material via PD-BaSO 4 particle functionalization as a local drug delivery system for avoiding the oral administration common in this kind of surgeries. Levofloxacin is used for fighting and preventing osteomyelitis, since it is a fluoroquinolone with anti-staphylococcal activity in osteoarticular tissues [38].…”

Section: Adsorption/release Test In 3d Printed Scaffoldsmentioning

confidence: 99%

“…To analyze the antibiotic efficacy of the 3D printed scaffolds the Agar Disk Diffusion tests against Staphylococcus aureus (S. aureus) were carried out. S. aureus was chosen because in bone infections is one of the most important pathogens due to its ability to adhere and form biofilms when in contact with tissue [38]. Figure 7 shows the Agar disk diffusion test corresponding to 3D printed scaffolds of PLA/PD-BaSO 4 with levofloxacin, PLA/PD-BaSO 4 scaffold as a negative control without levofloxacin and the disk of levofloxacin (5 µg) as a positive control.…”

Section: Antimicrobial Activity Of 3d Pla/pd-baso 4 Scaffolds With Lementioning

confidence: 99%

Benefits of Polydopamine as Particle/Matrix Interface in Polylactide/PD-BaSO4 Scaffolds

Sadaba

Larrañaga

Orpella‐Aceret

et al. 2020

IJMS

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This work reports the versatility of polydopamine (PD) when applied as a particle coating in a composite of polylactide (PLA). Polydopamine was observed to increase the particle–matrix interface strength and facilitate the adsorption of drugs to the material surface. Here, barium sulfate radiopaque particles were functionalized with polydopamine and integrated into a polylactide matrix, leading to the formulation of a biodegradable and X-ray opaque material with enhanced mechanical properties. Polydopamine functionalized barium sulfate particles also facilitated the adsorption and release of the antibiotic levofloxacin. Analysis of the antibacterial capacity of these composites and the metabolic activity and proliferation of human dermal fibroblasts in vitro demonstrated that these materials are non-cytotoxic and can be 3D printed to formulate complex biocompatible materials for bone fixation devices.

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Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection

Cited by 74 publications

References 48 publications

Anin vitrobiofilm model ofStaphylococcus aureusinfection of bone

Anin vitrobiofilm model ofStaphylococcus aureusinfection of bone

Mesoporous Silica‐Based Materials with Bactericidal Properties

Benefits of Polydopamine as Particle/Matrix Interface in Polylactide/PD-BaSO4 Scaffolds

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