Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces

Takahashi, Yasufumi; Shevchuk, Andrew; Novák, Pavel; Zhang, Yanjun; Ebejer, Neil; Macpherson, Julie V.; Unwin, Patrick R.; Pollard, Andrew J.; Roy, Debdulal; Clifford, Charles A.; Shiku, Hitoshi; Matsue, Tomokazu; Klenerman, David; Korchev, Yuri E.

doi:10.1002/anie.201102796

Cited by 260 publications

(320 citation statements)

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“…Starting from double barrel nanopipettes, one can selectively deposit carbon on either one 29 or both barrels. 49 The nanoelectrodes formed in this way can be independently functionalized with platinum ( Figure S8).…”

Section: Resultsmentioning

confidence: 99%

“…Pyrolytic carbon is selectively deposited on one barrel while leaving the other one unchanged. We employed these nanoprobes for simultaneous SICM-SECM imaging 29 and for the measurement of the electrochemical activity of the surface of living cells. 30 Here we describe the fabrication, characterization, and tailoring of carbon nanoelectrodes for intracellular electrochemical recordings.…”

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confidence: 99%

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Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microcopy probes which should allow high resolution electrochemical mapping of species on or in living cells.

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Section: Resultsmentioning

confidence: 99%

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Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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“…2,3 While the simplest nanopipettes contain just a single channel, multichannel devices are also possible, which increases the versatility of nanopipettes for nanoscience applications. 4,5 The channels can be open 5,6 (filled with electrolyte and a control electrode) or functionalized with deposited carbon, for example, to produce ultramicroelectrodes (UMEs) 7,8 that can also be further functionalized [9][10][11] to tune the sensory properties. Nanoelectrodes can also be fabricated by electrochemically plating nanopipettes with a variety of different metals.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Nanopipettes

et al. 2016

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Nanopipettes are widely used in electrochemical and analytical techniques as tools for sizing, sequencing, sensing, delivery and imaging. For all of these applications, the response of a nanopipette is strongly affected by its geometry and surface chemistry. As the size of nanopipettes becomes smaller, precise geometric characterization is increasingly important, especially if nanopipette probes are to be used for quantitative studies and analysis. This contribution highlights the combination of data from voltage-scanning ion conductivity experiments, transmission electron microscopy (TEM) and finite element method (FEM) simulations to fully characterize nanopipette geometry and surface charge characteristics, with an accuracy not achievable using existing approaches. Indeed, it is shown that presently used methods for nanopipette characterization can lead to highly erroneous information on nanopipettes. The new approach to characterization further facilitates high-level quantification of the behavior of nanopipettes in electrochemical systems, as demonstrated herein for a scanning ion conductance microscope (SICM) setup.

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“…Various SICM based positioning strategies have been developed over the years, to expand the range of substrates open to study. Moreover, the technique has been combined with complementary techniques including SECM [29][30][31][32] and near field scanning optical microscopy to enhance the information content. 33 Herein, we present an extremely quick and simple method to fabricate a hybrid pH-SICM probe, with an iridium oxide-coated electrode for pH sensing and an SICM barrel for distance control.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Dual Function Nanoscale pH-Scanning Ion Conductance Microscopy (SICM) Probes for High Resolution pH Mapping

et al. 2013

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(2013) Fabrication and characterization of dual function nanoscale pH-scanning ion conductance microscopy (SICM) probes for high resolution pH mapping. Analytical Chemistry, Volume 85 (Number 17). pp. 8070-8074 Permanent WRAP url: http://wrap.warwick.ac.uk/57249 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work of researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-forprofit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work http://dx.doi.org/10.1021/ac401883n The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. AbstractThe easy fabrication and use of nanoscale dual function pH-scanning ion conductance microscopy (SICM) probes is reported. These probes incorporate an iridium oxide coated carbon electrode for pH measurement and an SICM barrel for distance control, enabling simultaneous pH and topography mapping. These pH-SICM probes were fabricated rapidly from laser pulled theta quartz pipets, with the pH electrode prepared by in-situ carbon filling of one of the barrels by the pyrolytic decomposition of butane, followed by electrodeposition of a thin layer of hydrous iridium oxide. The other barrel was filled with an electrolyte solution and Ag/AgCl electrode as part of a conductance cell for SICM. The fabricated probes, with pH and SICM sensing elements typically on the 100 nm scale, were characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy and various electrochemical measurements. They showed a linear super-Nernstian pH response over a range of pH (pH 2-10). The capability of the pH-SICM probe was demonstrated by detecting both pH and topographical changes during the dissolution of a calcite microcrystal in aqueous solution. This system illustrates the quantitative nature of pH-SICM imaging, because the dissolution process changes the crystal height and interfacial pH (compared to bulk) and each is sensitive to the rate. Both measurements reve...

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Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces

Cited by 260 publications

References 44 publications

Electrochemical Nanoprobes for Single-Cell Analysis

Electrochemical Nanoprobes for Single-Cell Analysis

Characterization of Nanopipettes

Fabrication and Characterization of Dual Function Nanoscale pH-Scanning Ion Conductance Microscopy (SICM) Probes for High Resolution pH Mapping

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