Multifunctional nanofibrous scaffold for tissue engineering

Yang, Xiaochuan; Ogbolu, K.R.; Wang, H.

doi:10.1080/17458080701883707

Cited by 18 publications

(19 citation statements)

References 49 publications

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“…The use of an organic solvent during fabrication of TGF-β1-incorporated nanofibers may affect TGF-β1 bioactivity. However, based on our previous findings with fibronectin-incorporated nanofibers, [43] and consistent with the bioactivity results of this work (page 7, Figure 2), the effect was small. Once sufficient NFs were collected, the NFs were cut along the edges of the coverslip to assure full coverage of the entire coverslip surface.…”

Section: Methodssupporting

confidence: 92%

Micelle‐Coated, Hierarchically Structured Nanofibers with Dual‐Release Capability for Accelerated Wound Healing and Infection Control

Albright

Meng

Palanisamy

et al. 2018

Adv Healthcare Materials

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Tailoring nanofibrous matrices-a material with much promise for wound healing applications-to simultaneously mitigate bacterial colonization and stimulate wound closure of infected wounds is highly desirable. To that end, a dual-releasing, multiscale system of biodegradable electrospun nanofibers coated with biocompatible micellar nanocarriers is reported. For wound healing, transforming growth factor-β1 is incorporated into polycaprolactone/collagen (PCL/Coll) nanofibers via electrospinning and the myofibroblastic differentiation of human dermal fibroblasts is locally stimulated. To prevent infection, biocompatible nanocarriers of polypeptide-based block copolymer micelles are deposited onto the surfaces of PCL/Coll nanofibers using tannic acid as a binding partner. Micelle-modified fibrous scaffolds are favorable for wound healing, not only supporting the attachment and spreading of fibroblasts comparable to those on noncoated nanofibers, but also significantly enhancing fibroblast migration. Micellar coatings can be loaded with gentamicin or clindamycin and exhibit antibacterial activity as measured by Petrifilm and zone of inhibition assays as well as time-dependent reduction of cellular counts of Staphylococcus aureus cultures. Moreover, delivery time of antibiotic dosage is tunable through the application of a novel modular approach. Altogether, this system holds great promise as an infection-mitigating, cell-stimulating, biodegradable skin graft for wound management and tissue engineering.

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Section: Methodssupporting

confidence: 92%

Micelle‐Coated, Hierarchically Structured Nanofibers with Dual‐Release Capability for Accelerated Wound Healing and Infection Control

Albright

Meng

Palanisamy

et al. 2018

Adv Healthcare Materials

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“…With established electrospinning conditions [30, 31], the blend of PCL and collagen solutions was electrospun into nanofibers as shown in Fig. 2.…”

Section: Resultsmentioning

confidence: 99%

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair

Mahjour

Yang

et al. 2015

Burns

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Creation of functional skin substitutes within a clinically acceptable time window is essential for timely repair and management of large wounds such as extensive burns. The aim of this study was to investigate the possibility of fabricating skin substitutes via a bottom-up nanofiber-enabled cell assembly approach and using such substitutes for full-thickness wound repair in nude mice. Following a layer-by-layer (L-b-L) manner, human primary skin cells (fibroblasts and keratinocytes) were rapidly assembled together with electrospun polycaprolactone (PCL)/collagen (3:1 w/w, 8% w/v) nanofibers into 3D constructs, in which fibroblasts and keratinocytes were located in the bottom and upper portion respectively. Following culture, the constructs developed into a skin-like structure with expression of basal keratinocyte markers and deposition of new matrix while exhibiting good mechanical strength (as high as 4.0 MPa by 14 days). Treatment of the full-thickness wounds created on the back of nude mice with various grafts (acellular nanofiber meshes, dermal substitutes, skin substitutes and autografts) revealed that 14-day-cultured skin substitutes facilitated a rapid wound closure with complete epithelialization comparable to autografts. Taken together, skin-like substitutes can be formed by L-b-L assembling human skin cells and biomimetic nanofibers and they are effective to heal acute full-thickness wounds in nude mice.

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“…Considering that the major areas of the application of electrospinning currently are tissue engineering and controlled drug release [27–30], a bioresorbable polymer which is widely used for in vivo applications, i.e., a poly(caprolactone), PCL, was employed to investigate the dynamics of the hybrid extrusion/electrospinning process employing multiple nozzles. The PCL of our investigation was procured from Sigma‐Aldrich (St Louis, MO) and is reported to exhibit a number average molecular weight of 70,000–90,000, a melting point of 60°C and solid density of 1.145 g mL −1 at 25°C [31].…”

Section: Methodsmentioning

confidence: 99%

Dynamics of electrospinning of poly(caprolactone) via a multi‐nozzle spinneret connected to a twin screw extruder and properties of electrospun fibers

Senturk-Ozer

Ward

Gevgilili

et al. 2012

Polymer Engineering & Sci

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Conventional electrospinning is a simple process, suitable for the processing of solvated polymers at relatively low rates. It lacks the capabilities of conveying of solids, mixing of polymeric resins and devolatilization and has limited capabilities in melting and incorporation of fillers, including nanoparticles. The use of a twin screw extruder as the front end of the electrospinning process provides these capabilities but only becomes feasible at relatively high throughput rates. Such high rates of electrospinning can only be achieved by using multi‐nozzle spinnerets. However, the dynamics of electrospinning processes using multi‐nozzle spinnerets has not been well studied. Here a multi‐nozzle spinneret attached to a hybrid twin screw extrusion and electrospinning apparatus was employed for the characterization of the dynamics in terms of the electrospun mesh thickness distributions of poly(caprolactone), PCL, as a function of the principal parameters of the electrospinning process, i.e., applied voltage, distance of separation between the spinneret die, and the collector, i.e., a conductive mandrel, and its rotational speed. PCL fiber diameter and orientation distributions, as well as the thickness, crystallinity, and mechanical properties of the non‐woven meshes, were characterized to gain a basic understanding of how the electrospun mesh properties develop as a function of process parameters in the multi‐nozzle configuration. ENG. SCI., 2013. © 2012 Society of Plastics Engineers

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Multifunctional nanofibrous scaffold for tissue engineering

Cited by 18 publications

References 49 publications

Micelle‐Coated, Hierarchically Structured Nanofibers with Dual‐Release Capability for Accelerated Wound Healing and Infection Control

Micelle‐Coated, Hierarchically Structured Nanofibers with Dual‐Release Capability for Accelerated Wound Healing and Infection Control

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair

Dynamics of electrospinning of poly(caprolactone) via a multi‐nozzle spinneret connected to a twin screw extruder and properties of electrospun fibers

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