Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy

Zhang, Leilei; Xu, Jigeng; Sanders, Virginia M.; Letson, Alan D.; Roberts, Cynthia J.; Xu, Ronald X.

doi:10.1117/1.3457669

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…PLGA particles may be used to encapsulate absorption and fluorescence dyes in addition to a drug for multimodal imaging using fluorescence (FL), ultrasound (US), or photoacoustic tomography (PAT) [69]. Such multifunctional particles can also be formulated from a component material to reduce side effects of the encapsulated drug [70]. These particles can not only serve as a delivery system for the encapsulated drug but also reduce the harmful side effects through targeted drug delivery.…”

Section: Fabrication Techniques For Plga Carriersmentioning

confidence: 99%

Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier

2011

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In past two decades poly lactic-co-glycolic acid (PLGA) has been among the most attractive polymeric candidates used to fabricate devices for drug delivery and tissue engineering applications. PLGA is biocompatible and biodegradable, exhibits a wide range of erosion times, has tunable mechanical properties and most importantly, is a FDA approved polymer. In particular, PLGA has been extensively studied for the development of devices for controlled delivery of small molecule drugs, proteins and other macromolecules in commercial use and in research. This manuscript describes the various fabrication techniques for these devices and the factors affecting their degradation and drug release.

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Section: Fabrication Techniques For Plga Carriersmentioning

confidence: 99%

Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier

2011

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“…The microparticles contain a shell of PLGA, a FDA approved biodegradable polymer material, and a aqueous core that is capable of loading various water solvable drugs, antibodies, and genes. Such multifunctional microparticles can be used for various biomedical applications, including cancer therapy [26] and ophthalmologic disease treatment [27].…”

Section: Discussionmentioning

confidence: 99%

Coaxial electrospray for multimodal imaging and image-guided therapy

Zhang

et al. 2012

SPIE Proceedings

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Recent development in multimodal imaging and image-guided therapy requires multifunctional microparticles that encapsulate several imaging and therapeutic agents in the same carrier for simultaneous detection and treatment of the diseases. However, commonly used microfabrication processes for these microparticles have multiple limitations such as the low encapsulation efficiency and the loss of bioactivity for the encapsulated biological cargos. To overcome these limitations, we have carried out both the experimental and the theoretical studies on coaxial electrospray of poly(lactide-co-glycolide) PLGA microparticles. On the experimental side, a coaxial electrospray setup has been developed and tested. The setup consists of a customized coaxial needle assembly, two ring electrodes, two high-voltage power supplies, two syringe infusion pumps, a particle collection reservoir, and a process monitoring system. On the theoretical side, a classical normal mode method has been used for instability analysis of the coaxial electrified jet based on the experimental parameters. The effects of different dimensionless process parameters on the formation of different unstable modes have also been studied. The reported research represents the first step toward the quantitative control and optimization of the coaxial electrospray process for the fabrication of multifunctional microparticles in multimodal imaging and image-guided therapy.

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“…Currently, fluorophores and microbubbles attached with various functional groups have been widely reported in the literature and many of them are available commercially. 39,[46][47][48] Thus, the synthesis of microbubble-based UMF contrast agents become straightforward, which is necessary for bringing this technique into practical application. More importantly, simultaneous multimolecule targeted imaging becomes straightforward by using fluorophores with different excitation and emission wavelengths, which will be investigated in the future.…”

Section: Further Discussion About the Fluorescent Microbubbles And Tmentioning

confidence: 99%

Ultrasound-modulated fluorescence based on fluorescent microbubbles

et al. 2014

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Abstract. Ultrasound-modulated fluorescence (UMF) imaging has been proposed to provide fluorescent contrast while maintaining ultrasound resolution in an optical-scattering medium (such as biological tissue). The major challenge is to extract the weakly modulated fluorescent signal from a bright and unmodulated background. UMF was experimentally demonstrated based on fluorophore-labeled microbubble contrast agents. These contrast agents were produced by conjugating N-hydroxysuccinimide (NHS)-ester-attached fluorophores on the surface of amine-functionalized microbubbles. The fluorophore surface concentration was controlled so that a significant self-quenching effect occurred when no ultrasound was applied. The intensity of the fluorescent emission was modulated when microbubbles were oscillated by ultrasound pulses, presented as UMF signal. Our results demonstrated that the UMF signals were highly dependent on the microbubbles' oscillation amplitude and the initial surface fluorophore-quenching status. A maximum of ∼42% UMF modulation depth was achieved with a single microbubble under an ultrasound peak-to-peak pressure of 675 kPa. Further, UMF was detected from a 500-μm tube filled with contrast agents in water and scattering media with ultrasound resolution. These results indicate that ultrasound-modulated fluorescent microbubble contrast agents can potentially be used for fluorescence-based molecular imaging with ultrasound resolution in the future.

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Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy

Cited by 13 publications

References 14 publications

Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier

Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier

Coaxial electrospray for multimodal imaging and image-guided therapy

Ultrasound-modulated fluorescence based on fluorescent microbubbles

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