Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics

Mabrouk, Mostafa; El-Wahab, Rasha M. Abd; Beherei, Hanan H.; Selim, M.M.; Das, Diganta Bhusan

doi:10.1016/j.ijpharm.2020.119658

Cited by 27 publications

(14 citation statements)

References 56 publications

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“…OD 625 is an absorbance measurement at a wavelength of 625 nm in an accuSkan Go spectrophotometer (Fisher Scientific, USA). MIC is defined as the lowest concentration of an antimicrobial that will inhibit the visible growth by~90% after 24 h of incubation at 37 • C compared with a growth control [38]. These values were determined by broth dilutions in a 96-well microtiter plate format consisting of ten concentrations (0-5000 mg/L) in triplicates of magnetite NPs.…”

Section: Determination Of Minimal Inhibitory Concentration (Mic) Of Magnetite Npsmentioning

confidence: 99%

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

et al. 2021

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Both ionic and nanoparticle iron have been proposed as materials to control multidrug-resistant (MDR) bacteria. However, the potential bacteria to evolve resistance to nanoparticle bacteria remains unexplored. To this end, experimental evolution was utilized to produce five magnetite nanoparticle-resistant (FeNP1–5) populations of Escherichia coli. The control populations were not exposed to magnetite nanoparticles. The 24-h growth of these replicates was evaluated in the presence of increasing concentrations magnetite NPs as well as other ionic metals (gallium III, iron II, iron III, and silver I) and antibiotics (ampicillin, chloramphenicol, rifampicin, sulfanilamide, and tetracycline). Scanning electron microscopy was utilized to determine cell size and shape in response to magnetite nanoparticle selection. Whole genome sequencing was carried out to determine if any genomic changes resulted from magnetite nanoparticle resistance. After 25 days of selection, magnetite resistance was evident in the FeNP treatment. The FeNP populations also showed a highly significantly (p < 0.0001) greater 24-h growth as measured by optical density in metals (Fe (II), Fe (III), Ga (III), Ag, and Cu II) as well as antibiotics (ampicillin, chloramphenicol, rifampicin, sulfanilamide, and tetracycline). The FeNP-resistant populations also showed a significantly greater cell length compared to controls (p < 0.001). Genomic analysis of FeNP identified both polymorphisms and hard selective sweeps in the RNA polymerase genes rpoA, rpoB, and rpoC. Collectively, our results show that E. coli can rapidly evolve resistance to magnetite nanoparticles and that this result is correlated resistances to other metals and antibiotics. There were also changes in cell morphology resulting from adaptation to magnetite NPs. Thus, the various applications of magnetite nanoparticles could result in unanticipated changes in resistance to both metal and antibiotics.

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Section: Determination Of Minimal Inhibitory Concentration (Mic) Of Magnetite Npsmentioning

confidence: 99%

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

et al. 2021

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“…According to previous studies, the main challenge of Fe 3 O 4 nanoparticles in drug delivery is their tendency to agglomerate; therefore, these nanoparticles are unstable and cannot be dispersed very well . Agglomeration of Fe 3 O 4 nanoparticles occurs due to the high surface area and the presence of magnetic dipole–dipole interactions or van der Waal forces . This defect in Fe 3 O 4 nanoparticles significantly reduces the drug release efficiency compared to Fe 3 O 4 /CQD nanocomposites.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Study: Synthesis and Evaluation of Fe₃O₄/CQD Magnetic/Fluorescent Nanocomposites for Targeted Drug Delivery, MRI, and Cancer Cell Labeling Applications

et al. 2022

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In the present study, first, Fe3O4 nanoparticles were functionalized using glutaric acid and then composited with CQDs. Doxorubicin (DOX) drug was loaded to evaluate the performance of the nanocomposite for targeted drug delivery applications. The XRD pattern confirmed the presence of characteristic peaks of CQDs and Fe3O4. In the FTIR spectrum, the presence of carboxyl functional groups on Fe3O4/CQDs was observed; DOX (positive charge) is loaded onto Fe3O4/CQDs (negative charge) by electrostatic absorption. FESEM and AFM images showed that the particle sizes of Fe3O4 and CQDs were 23–75 and 1–3 nm, respectively. The hysteresis curves showed superparamagnetic properties for Fe3O4 and Fe3O4/CQDs (57.3 and 8.4 emu/g). The Fe3O4 hysteresis curve showed superparamagnetic properties (Ms and Mr: 57.3 emu/g and 1.46 emu/g. The loading efficiency and capacity for Fe3O4/CQDs were 93.90% and 37.2 mg DOX/g MNP, respectively. DOX release from Fe3O4/CQDs in PBS showed pH-dependent release behavior where after 70 h at pH 5 and 7.4, about 50 and 21% of DOX were released. Fluorescence images of Fe3O4/CQD-treated cells showed that Fe3O4/CQDs are capable of labeling MCF-7 and HFF cells. Also, T2-weighted MRI scans of Fe3O4/CQDs in water exhibited high r 2 relaxivity (86.56 mM–1 S–1). MTT assay showed that DOX-loaded Fe3O4/CQDs are highly biocompatible in contact with HFF cells (viability = 95%), but they kill MCF-7 cancer cells (viability = 45%). Therefore, the synthesized nanocomposite can be used in MRI, targeted drug delivery, and cell labeling.

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“…OD625 is an absorbance measurement at a wavelength of 625 nm in an accuSkan Go spectrophotometer (Fisher Scientific, USA). MIC is defined as the lowest concentration of an antimicrobial that will inhibit the visible growth by ~90% after 24 h of incubation at 37°C compared with a growth control [38]. These values were determined by broth dilutions in a 96-well microtiter plate format consisting of ten concentrations (0-5000 mg/L) in triplicates of magnetite NPs.…”

Section: Determination Of Minimal Inhibitory Concentration (Mic) Of Mmentioning

confidence: 99%

Experimental Evolution of Magnetite Nanoparticle Resistance in <em>Escherichia coli</em>

Ewunkem¹,

Rodgers²,

Campbell³

et al. 2021

Preprint

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Experimental evolution was utilized to produce 5 magnetite nanoparticle-resistant (FeNP1-5) populations of Escherichia coli. The control populations were not exposed to magnetite nanoparticles. The 24-hour growth of these replicates was evaluated in the presence of increasing concentrations magnetite NPs as well as other ionic metals (gallium III, iron II, iron III, silver I) and antibiotics (ampicillin, chloramphenicol, rifampicin, sulfanilamide, tetracycline). Scanning electron microscope was utilized to determine cell size and shape in response to magnetite nanoparticle selection. Whole genome sequencing was carried out to determine if any genomic changes that resulted from magnetite nanoparticle resistance. After 25 days of selection magnetite resistance was evident in the FeNP treatment. The FeNP populations also showed a highly significantly (p &lt; 0.0001) greater 24-growth as measured by optical density in metals (Fe (II), Fe (III), Ga (III), Ag and Cu II); as well as antibiotics (ampicillin, chloramphenicol, rifampicin, sulfanilamide, and tetracycline). The FeNP resistant populations also showed a significantly greater cell length compared to controls (p &lt; 0.001). Genomic analysis of FeNP identified both polymorphisms and hard selective sweeps in the RNA polymerase genes rpoA, rpoB, and rpoC. Collectively, our results show that E. coli can rapidly evolve resistance to magnetite nanoparticles and that this result is correlated resistances to other metals and antibiotics. There were also changes in cell morphology resulting from adaptation to magnetite NPs. Thus, the various applications of magnetite nanoparticles could result in unanticipated changes in resistance to both metal and antibiotics.

show abstract

Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics

Cited by 27 publications

References 56 publications

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

In Vitro Study: Synthesis and Evaluation of Fe₃O₄/CQD Magnetic/Fluorescent Nanocomposites for Targeted Drug Delivery, MRI, and Cancer Cell Labeling Applications

Experimental Evolution of Magnetite Nanoparticle Resistance in <em>Escherichia coli</em>

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Multifunctional magnetite nanoparticles for drug delivery: Preparation, characterisation, antibacterial properties and drug release kinetics

Cited by 27 publications

References 56 publications

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

In Vitro Study: Synthesis and Evaluation of Fe3O4/CQD Magnetic/Fluorescent Nanocomposites for Targeted Drug Delivery, MRI, and Cancer Cell Labeling Applications

Experimental Evolution of Magnetite Nanoparticle Resistance in <em>Escherichia coli</em>

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In Vitro Study: Synthesis and Evaluation of Fe₃O₄/CQD Magnetic/Fluorescent Nanocomposites for Targeted Drug Delivery, MRI, and Cancer Cell Labeling Applications