Multifunctional magnetic cargo-complexes with radical scavenging properties

Lungoci, Ana-Lacramioara; Turin-Moleavin, Ioana-Andreea; Corciovă, Andreia; Mircea, Cornelia; Arvinte, Adina; Fifere, Adrian; Marangoci, Narcisa; Pinteală, Mariana

doi:10.1016/j.msec.2018.10.013

Cited by 13 publications

(22 citation statements)

References 36 publications

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“…Magnetite nanoparticles (MNP) were synthetised following the classical method of co-precipitation, from a mixture of ferric and ferrous salts at high pH [11,32]. Further, based upon the previous experience [6], the fresh magnetite nanoparticles were coated with PEI by a simple mixing of the MNP aqueous dispersion with the PEI solution. At this stage the MPEI nanoparticles are stabilised by physical interactions, such as electrostatic interactions, given by the PEI polycation and MNPs with negative zeta-potential, becoming stable in all physicochemical measurements [6].…”

Section: Resultsmentioning

confidence: 99%

“…The obtained MNPs were washed several times with water and ethanol. The bare MNPs were stored in ethanol at 5 °C in the refrigerator prior to use.Preparation of core-shell MNP coated with PEI (MPEI) was done by a previously reported method, without any modification [6]. In brief, 10 mL of PEI (10%, 1.8 kDa) solution was added to 8 mL suspension of fresh MNP (50 mg/mL) in deionised water, sonicated for 5 min and stirred for 24 h. Excess of PEI was removed by washing the product five times with water using magnetic decantation.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, core-shell magnetic nanoparticles (MNPs) are combined with gold (Au) [3], silver (Ag) [4] and titanium oxide (TiO 2 ) [5] nanoparticles in order to obtain nanoconjugates with specific properties of the two types of nanoparticles. In this respect, core-shell magnetic nanoparticles (MNPs) can be successfully used as agents for transport and delivery (at the desired site) of an antioxidant drug capable of inactivating reactive oxygen species (ROS) and/or reactive nitrogen species (RNS), hindering the side effects that may occur, such as DNA mutations and lipid peroxidation [6,7,8,9]. Over-production of reactive species limits the antioxidant defences of the living body that causes the occurrence of numerous disorders, such as cancer, as well as cardiovascular, inflammatory and neurodegenerative diseases [8].…”

Section: Introductionmentioning

confidence: 99%

“…Hyperbranched polyethylenimine (PEI) is a well-known and intensively studied biocompatible polymer, and it is tested and used in bio-applications, such as being a transfection reagent for drug delivery systems or tissue culture [27,28,29]. As recently demonstrated by our group, 1.8 kDa-branched PEI is able to form a shell around the magnetite nanoparticle, through electrostatic interactions, and quickly facilitating the loading of the protocatechuic acid, even of its inclusion complex with sulfobutylether-β-cyclodextrin, to deliver the natural antioxidant able to scavenge free radicals [6].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Turin-Moleavin¹,

Fifere²,

Lungoci³

et al. 2019

Nanomaterials

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Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Turin-Moleavin¹,

Fifere²,

Lungoci³

et al. 2019

Nanomaterials

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“…The macrocyclic route became a frequent strategy in the encapsulation of various natural or synthetic antioxidants which suffer from solubility issues. The PEI-based shell increases the stability and it assists the loading, transport, and delivery of the antioxidant, leading to the formation of drug delivery vehicles that fuse the bio-useful traits of the core-shell construct and its load [139].…”

Section: Metallic Nanoconjugates For Biomedical Applicationsmentioning

confidence: 99%

Smart Supra- and Macro-Molecular Tools for Biomedical Applications

2020

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Stimuli-responsive, “smart” polymeric materials used in the biomedical field function in a bio-mimicking manner by providing a non-linear response to triggers coming from a physiological microenvironment or other external source. They are built based on various chemical, physical, and biological tools that enable pH and/or temperature-stimulated changes in structural or physicochemical attributes, like shape, volume, solubility, supramolecular arrangement, and others. This review touches on some particular developments on the topic of stimuli-sensitive molecular tools for biomedical applications. Design and mechanistic details are provided concerning the smart synthetic instruments that are employed to prepare supra- and macro-molecular architectures with specific responses to external stimuli. Five major themes are approached: (i) temperature- and pH-responsive systems for controlled drug delivery; (ii) glycodynameric hydrogels for drug delivery; (iii) polymeric non-viral vectors for gene delivery; (iv) metallic nanoconjugates for biomedical applications; and, (v) smart organic tools for biomedical imaging.

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