Multifunctional Iron Bound Lactoferrin and Nanomedicinal Approaches to Enhance Its Bioactive Functions

Kanwar, Jagat R.; Roy, Kislay; Patel, Yogesh; Zhou, Shu-Feng; Singh, Manju Rawat; Singh, Deependra; Nasir, Muhammad; Sehgal, Rakesh; Sehgal, Alka; Singh, Ram Sarup; Garg, Sanjay; Kanwar, Rupinder K.

doi:10.3390/molecules20069703

Cited by 104 publications

(82 citation statements)

References 170 publications

(198 reference statements)

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“…Tests carried out on mandarins with bLfcin and Lf (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31) showed a significant protection against Penicillium digitatum at a concentration close to the in vitro MIC value [115]. More recently Baruzzi et al demonstrated the interesting activity of bLfcin in controlling microbial spoilage in ready-to-eat leafy vegetables during cold storage [116].…”

Section: Fruits and Vegetablesmentioning

confidence: 99%

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Bruni¹,

et al. 2016

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Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. Among AMP sources, an interesting class regards the food-derived bioactive agents. The whey protein lactoferrin (Lf) is an iron-binding glycoprotein that plays a significant role in the innate immune system, and is considered as an important host defense molecule. In search for novel antimicrobial agents, Lf offers a new source with potential pharmaceutical applications. The Lf-derived peptides Lf(1-11), lactoferricin (Lfcin) and lactoferrampin exhibit interesting and more potent antimicrobial actions than intact protein.Particularly, Lfcin has demonstrated strong antibacterial, anti-fungal and antiparasitic activity with promising applications both in human and veterinary diseases (from ocular infections to osteo-articular, gastrointestinal and dermatological diseases).

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Section: Fruits and Vegetablesmentioning

confidence: 99%

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Bruni¹,

et al. 2016

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“…Lf has been described as modulator of the immune system, particularly regulating the production of proinflammatory cytokines [23]. bLf has shown beneficial effects when tested in patients with cancer [24][25][26], and some studies have shown that Lf can promote wound healing and bone growth [5]. bLf showed an immunostimulant effect in calves [27,28], chickens [29], and fishes [30,31].…”

Section: Introductionmentioning

confidence: 99%

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Luna-Castro¹,

Samaniego-Barrón²,

Serrano-Rubio³

et al. 2017

J Adv Dairy Res

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Lactoferrin (Lf) is an iron-chelating glycoprotein present in milk and mucosal secretions, a component of the mammalian innate immune system. Lf is microbiostatic and microbicidal. Lf can reduce the bacterial expression of virulence factors, such as those involved in biofilm production and protease secretion. The high identity among mammalian Lf sequences facilitates its use in human and veterinary medicine. Lf of bovine origin is the principal Lf used due to its commercial availability through purification from milk whey; recombinant Lfs (bovine, human, and porcine) have been used as well. Lf is a stable protein that retains its physicochemical characteristics under gastric pH conditions, and in most cases it is bioactive even after digestion; thus, the incorporation of Lf into diets facilitates its administration to animals. The aim of this review is to examine original research in which the effects of bovine and porcine Lf on pathogens of domestic animals have been demonstrated through in vitro and in vivo assays, with the purpose of ascertaining the benefits that Lf provides in the treatment of infectious diseases.

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“…The alginate coating is not dissolved at lower pH in stomach but dissolve at higher pH (.6) in the intestine and due to mucoadhesive nature of the chitosan the remaining CCo-CPbLf NCs stick to the intestine and pass through due to endocytosis and/or transcytosis. 27 The efficacy of these NCs has already been studied against bacterial infection and cancer. Activity of the loaded Lf protein as well as efficacy has been shown previously by Kanwar et al and was found to be present in blood plasma at a concentration of 1,000 ng/mL after 8 hours postadministration and up to 100 ng/mL at 10 days postadministration, which has been found to be optimum to inhibit any tumor or microbial infection.…”

mentioning

confidence: 99%

Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

Anand¹,

Sehgal²,

Kanwar³

et al. 2015

IJN

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Toxoplasma gondii is a deadly intracellular parasite known to reside in every nucleated cell and known to cause severe complications in immunocompromised host. Standard drugs are cost effective and cause side effects, therefore, there is a necessity for a new drug molecule with immunomodulatory potential. Lactoferrin (Lf) is a natural milk protein, which has shown antimicrobial properties in its nanoformulation using alginate chitosan calcium phosphate bovine lactoferrin nanocapsules (AEC-CCo-CP-bLf-NCs). The present study was aimed to analyze and compare the effect of bovine Lf (bLf) in its native as well as nanoformulation (AEC-CCo-CP-bLf-NC) against coccidian parasite T. gondii. In vitro analysis has shown a significant increase in nitric oxide production and low parasitemia in in vitro cell culture model. In vivo BALB/c mice model have been used to develop human toxoplasmosis model. After treatment with NCs it has substantially increased the bioavailability of the protein and showed comparatively increased levels of reactive oxygen species, nitric oxide production, and Th1 cytokine which helped in parasite clearance. The mechanism of action of NCs has been clarified by immunoreactivity analysis, which showed accumulation of Lf in macrophages of various visceral organs, which is the site of parasite multiplication. Effect of NCs has significantly decreased ( P <0.05) the parasite load in various organs and helped survival of mice till day 25 postinfection. Fe metabolism inside the mice has been found to be maintained even after administration of mono form of Lf, this indicates novelty of Lf protein. From the present study we concluded that nanoformulation did not reduce the therapeutic potential of Lf protein; however, nanoformulation has enhanced the stability of the protein and shown anti-toxoplasmal activity. Our study presents for the first time nanoformulation of Lf protein against Toxoplasma , which has advantages over the standard drug therapy without any side effects.

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Multifunctional Iron Bound Lactoferrin and Nanomedicinal Approaches to Enhance Its Bioactive Functions

Cited by 104 publications

References 170 publications

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

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