Multifunctional GQDs-Concanavalin A@Fe3O4 nanocomposites for cancer cells detection and targeted drug delivery

Chowdhury, Ankan Dutta; Ganganboina, Akhilesh Babu; Tsai, Yuan-Chung; Chiu, Hsin‐Cheng; Doong, Ruey‐an

doi:10.1016/j.aca.2018.04.029

Cited by 68 publications

(37 citation statements)

References 56 publications

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“…In the presence of a magnetic field, the DOX concentration in HeLa cells was more than double. In addition, HeLa cells were 13% more sensitive to DOX in this system than normal cells, confirming the selective role of concanavalin A [167]. The in vitro screening of multifunctional hydrophilic magnetic NPs based on egg yolk-functionalized Pluronic™ F-127 (GYSMNP@PF127), with a particle size of 180 nm, a negative surface charge, and high hemocompatibility, showed a high capacity of 91% w/w for DOX, with a high heating effect with an alternating (AC) magnetic field (internal energy loss in the range of 2.1 to 2.7 nHm 2 /kg), a controlled release of the pH responsive drug, and thermal stimulus (46% at the acidic pH of the tumor and 7% at the physiological pH) when in an alternating magnetic field (MF) [168].…”

Section: Figure 11supporting

confidence: 62%

Graphenic Materials for Biomedical Applications

2019

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Graphene-based nanomaterials have been intensively studied for their properties, modifications, and application potential. Biomedical applications are one of the main directions of research in this field. This review summarizes the research results which were obtained in the last two years (2017-2019), especially those related to drug/gene/protein delivery systems and materials with antimicrobial properties. Due to the large number of studies in the area of carbon nanomaterials, attention here is focused only on 2D structures, i.e. graphene, graphene oxide, and reduced graphene oxide.

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Section: Figure 11supporting

confidence: 62%

Graphenic Materials for Biomedical Applications

2019

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“…Owing to these properties, carbon dots (CDs) have tremendous potential to be used in a number of applications such as cell imaging and sensing [4][5][6]. Fluorescence carbon nanoparticle (CNP) shows high potential in biological labeling, bio-imaging and other different optoelectronic device application [7,8], targeted drug delivery [9,10] in cancer theranostics [11,12], and drug delivery [13]. In addition to this, they find multiple applications in analytical chemistry [14].…”

Section: Introductionmentioning

confidence: 99%

Blue light-emitting carbon dots (CDs) from a milk protein and their interaction with Spinacia oleracea leaf cells

Bajpai

D'Souza²,

Suhail

2019

Int Nano Lett

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The milk protein casein (Cas) has been employed as carbon resource material to synthesize nitrogen-doped carbon dots (N-CDs) via microwave exposure. The dots, when exposed to UV light, produced blue fluorescence. The N-CDs were characterized by ultra violet (UV) spectroscopy, Fourier transformation infrared spectroscopy, X-ray diffraction (XRD), dynamic light scattering analysis, fluorescent microscopy (FM), and transmission electron microscopy (TEM). The XRD analysis revealed a broad peak at 2θ = 20°, thus indicating the turbostratic carbon phase. TEM analysis and particle size distribution curve revealed that nearly, 85% of the particles had diameter below 10 nm and the particles had spherical geometry. The HRTEM analysis revealed that carbon dots exhibited lattice fringes with a d-spacing of 0.21 nm, corresponding to the (100) plane lattice of graphite. The fluorescence spectral studies indicated a red shift in the emission peak from 420 to 450 nm as the excitation wavelength increased from 300 to 340 nm. The zeta potential of particles was found to be -11.3 mV. Finally, impregnation of N-CDs was studied in Spinacia oleracea leaf. It was observed that as the concentration of N-CDs' solution increased, percent insertion (PI) also increased, but the time required for maximal insertion decreased with increasing concentrations of N-CDs in the feed solutions. In the carbon dots' solution with a concentration of 200 ppm, maximum percent insertion (MPI) was obtained after 80 min. However, with the increasing concentration of N-CDs in the feed solutions, time of getting MPI reduced, i.e., in 600 ppm, it was 30 min, and in 800 ppm, it was 10 min.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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“…Some labelling techniques report the recognition of carbohydrates in fungi, bacteria, or cancer cells using traditional methods. It has also been found that labelling is improved using coupling agents such as EDC or sulfo-NHS [51][52][53], although the physicochemical characteristics of the lectin-QD complex have not been fully described. To date, no studies have reported the coupling of a lectin with QD using a microfluidic platform.…”

Section: Discussionmentioning

confidence: 99%

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

et al. 2020

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Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The average particle size (380 nm) and the average zeta potential (−18.51 mV) were determined. The cytotoxicity of the TBL-IE2 and TBL-IE2-QD was assayed on HT-29 colon cancer cells, showing no differences between them (p ≤ 0.05), where the LC50 values were 1.0 × 10−3 and 1.7 × 10−3 mg/mL, respectively. The microfluidic technique allowed control of the coupling between the QD and the protein, substantially improving the labelling process, providing a rapid and efficient method that enabled the traceability of lectins. Future studies will focus on the potential use of the QD-labelled lectin to recognize tumor tissues.

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Multifunctional GQDs-Concanavalin A@Fe3O4 nanocomposites for cancer cells detection and targeted drug delivery

Cited by 68 publications

References 56 publications

Graphenic Materials for Biomedical Applications

Graphenic Materials for Biomedical Applications

Blue light-emitting carbon dots (CDs) from a milk protein and their interaction with Spinacia oleracea leaf cells

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

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