Multifunctional gentamicin supplementation of poly(γ‐glutamic acid)‐based hydrogels for wound dressing application

Abstract

The process of wound healing is composed of coagulation, inflammation, fibroplasia, collagenation, epithelization, and wound contraction. The wound dressing should protect the wound from bacterial infection, maintain a moist healing environment, and promote cell migration to reconstruct damaged tissue, and be easy to apply and remove to improve patient comfort. The purpose of our study was to develop multifunctional hydrogels composed of genipin-crosslinked biodegradable biomaterials of poly(c-glutamic acid) and gelatin, encapsulating gentamicin to accelerate wound healing. The results of swelling ratio measurements clearly indicate that hydrogel composition of poly(c-glutamic acid)-gelatin had a higher swelling ratio and lower peel adhesion properties than gelatin hydrogel alone. In an in vitro study, the gentamicin incorporated in prepared hydrogels effectively inhibited target microorganisms and produced a higher expression of Type I collagen in fibroblast cells. Confocal laser scanning microscopy revealed that the fibroblast cells cultured in the hydrogel membranes produced fibroblast cell migration and showed a continuous lined cytoskeletal distributing status. In the in vivo study, it was found that the gentamicin incorporated in genipin-crosslinked c-PGA-gelatin wound dressing demonstrates the potential of such biologically functionalized dressing to accelerate wound closure and, hence, its potential clinical usefulness.

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

“…is for a protonated amino group (-NH 3 + ) or N-H (amide) [30,31]. The characteristic peak assigned to the aliphatic side chain of gelatin is at 2970 cm −1 [32].…”

Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

“…The ideal wound dressing protects wound tissue from bacterial infection, reduces inflammation, and induces cell proliferation to aid in reconstruction of damaged tissue [26]. The primary aim of this work was to develop a multifunctional bi-layer wound dressing system composed of gentamicin incorporated within a c-PGA/gelatin membrane to prevent bacterial infection and within activated carbon fibers to promote fibroblast cell growth and cell phenotype maintenance (Fig.…”

“…Second, gentamicin was incorporated into a genipincrosslinked c-PGA/gelatin membrane. The preparation protocol used was as previously described, but with the following modifications [26]. A stock solution of gelatin (20.0 %, w/v) in PBS was prepared by dissolving 1 g of gelatin in 5 mL PBS and stirring for 3 h at 50°C.…”

Biomaterials Made from Coiled-Coil Peptides

“…Moreover, hydrogels are often highly biocompatible due to their capacity for water absorption, physical elasticity, and relative softness (Kopeček 2007, Yao et al 2014. The aforementioned qualities make hydrogels a convincing substitute for native extracellular matrix (Slaughter et al 2009;Yao et al 2014), as well as suitable candidates for cell culture applications (Frampton et al 2011;Jung et al 2011), biomedical uses such as wound treatment (Wong et al 2011;Lin et al 2011), and drug delivery (Garbern et al 2011;Guziewicz et al 2011). Unsurprisingly, hydrogels were the first biomaterials developed for medical uses in humans (Kopeček Fig.…”