Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing

Abstract

This work developed a novel bi-layer wound dressing composed of 3D activated carbon fibers that allows facilitates fibroblast cell growth and migration to a wound site for tissue reconstruction, and the gentamicin is incorporated into a poly(γ-glutamic acid)/gelatin membrane to prevent bacterial infection. In an in vitro, field emission scanning electron microscopy shows that rat skin fibroblasts appeared and spread on the surface of activated carbon fibers, and penetrated the interior and exterior of the 3D activated carbon fiber construct to a depth of roughly 200 μm. An in vivo analysis shows that fibroblast cells containing the proposed 3D scaffold had the potential of a biologically functionalized dressing to accelerate wound closure. Additionally, fibroblasts migrated to the wound site in a bi-layer wound dressing containing fibroblasts, enhancing fibronectin and type I collagen expression, resulting in faster skin regeneration than that achieved with a Tegaderm™ hydrocolloid dressing or gauze.

A poly(γ‐glutamic acid)‐based hydrogel loaded with superoxide dismutase for wound healing

“…At day 21, re‐epithelialization in the wounds treated with SOD‐PGAS/PGA‐H was more complete than re‐epithelialization in those treated with PGAS/PGA‐H, while the surface of the wounds in control group was covered with underlying granulated tissue that had not yet epithelialized. Previously reported results have shown that appropriate inflammation is necessary for wound healing, but prolonged inflammation is detrimental to its outcome . This observation is consistent with our results, where wound healing in the test groups (PGAS/PGA‐H and SOD‐PGAS/PGA‐H), especially in the SOD‐PGAS/PGA‐H group, was faster and more complete than in the control group, probably due to the fact that wounds re‐epithelialize more rapidly under moist conditions .…”

Preparation of epigallocatechin gallate-loaded nanoparticles and characterization of their inhibitory effects onHelicobacter pylorigrowthin vitroandin vivo

“…The optical density was read with a microplate spectrofluorometer (Molecular Devices SpectraMax M2 e , Sunnyvale, USA) at a wavelength of 570 nm. All experiments were performed six times with eight replicate wells for each sample and control per assay [34, 35]. …”

Utility of Carbon Fiber Implants in Orthopedic Surgery: Literature Review

“…This scaffold promoted fibroblast growth and migration, leading to up-regulation of fibronectin and type I collagen, which was theorized to have allowed for accelerated wound healing and closure. [16] …”