Multifunctional Encoded Particles for High-Throughput Biomolecule Analysis

Abstract

High-throughput screening for genetic analysis, combinatorial chemistry, and clinical diagnostics benefits from multiplexing, which allows for the simultaneous assay of several analytes but necessitates an encoding scheme for molecular identification. Current approaches for multiplexed analysis involve complicated or expensive processes for encoding, functionalizing, or decoding active substrates (particles or surfaces) and often yield a very limited number of analyte-specific codes. We present a method based on continuous-flow lithography that combines particle synthesis and encoding and probe incorporation into a single process to generate multifunctional particles bearing over a million unique codes. By using such particles, we demonstrate a multiplexed, single-fluorescence detection of DNA oligomers with encoded particle libraries that can be scanned rapidly in a flow-through microfluidic channel. Furthermore, we demonstrate with high specificity the same multiplexed detection using individual multiprobe particles.

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

“…Hydrogel microparticles can be prepared by using either emulsion templated polymerization or lithographic techniques. 28,[31][32][33] We chose the former method to achieve a high yield. In a typical synthesis, Acrydite-modified Hg 2+ -binding DNA, acrylamide, and bisacrylamide were mixed.…”

Design Advances in Particulate Systems for Biomedical Applications

“…[ 278 ] Disk-shaped Janus particles composed by a fl uorescent graphically encoded region and a probe-loaded region enabled detection of specifi c DNA sequences and could also be used in high throughput analyses. [ 279 ] The same concept was used to multiplex detection of proteins. [ 280 ] PEG-based microparticles were obtained having a distinctive graphical code (unpolymerized holes in the wafer structure of the microparticles).…”

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

“…[27][28][29][30] Strategies avoiding such 2D fixation of various hydrogels have employed shape-coded hydrogel particles as a suspension microarray format for multiplexed bioassays. 31,32 Here we describe a method for the rapid fabrication of alginate hydrogel particles of defined sizes and shapes using the effect of discontinuous dewetting on an SH-SL microarray combined with the sandwiching method to achieve the simultaneous gelation of the pre-hydrogel droplets.…”