Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

Neto, Ana I.; Demir, Konstantin; Popova, Anna A.; Oliveira, Mariana B.; Mano, J. F.; Levkin, Pavel A.

doi:10.1002/adma.201602350

Cited by 85 publications

(93 citation statements)

References 39 publications

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“…Such hydrogel compartmentalization can be readily designed from various stimuliresponsive polymers, imparting the microarrays with responsiveness/adaptability to environmental changes. [22][23][24][25][26] In this study we exploit supramolecular building units to construct patterned hydrogel microcapsules on solid substrates for cargo delivery and chemical sensing. [17][18][19] Patterned arrays made of hydrogel microcapsules have been rarely reported, due to the difficulty of accumulating polymer backbones at the microdroplet surface during the gelation process, unless a multiple emulsion technique is used.…”

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confidence: 99%

Patterned Arrays of Supramolecular Microcapsules

Zhang

Liu

et al. 2018

Adv Funct Materials

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Micropatterning of hydrogel has brought innovative outcomes in fundamental and applied material sciences. Previous approaches have mainly been dedicated to fabricate arrays of bulk hydrogel beads, which have inherent challenges including loading ability, scalability, specificity, and versatility. Here, a methodology is presented to create hollow microcapsule arrays from sessile microdroplets. The difference in wettability between hydrophilic and hydrophobic surfaces enables self-partitioning of liquid into microdroplet arrays, serving as microreservoirs to load complementarily functionalized host-guest polymers, cucurbit[8]uril-threaded highly branched polyrotaxanes (HBP-CB[8]) and naphthyl-functionalized hydroxyethyl cellulose (HEC-Np). The interfacial dynamic complexation between positively charged HBP-CB[8] and HEC-Np occurs in the presence of negatively charged surfactants, resulting in con-densed supramolecular hydrogel skins. The hydrogel microcapsules are uniform in size and are developed to encapsulate target cargos in a robust and well-defined manner. Moreover, the microcapsule substrates are further used for surface enhanced Raman spectroscopy sensing upon loading of gold nanoparticles. This facile assembly of microcapsule arrays has potential applications in controlled cargo delivery, bio-sensing, high-throughput analysis, and sorting.

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confidence: 99%

Patterned Arrays of Supramolecular Microcapsules

Zhang

Liu

et al. 2018

Adv Funct Materials

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“…To engineer the miniaturized structures for cell culture, so-called building blocks, different microfabrication strategies have been explored (Custodio et al, 2015) (Mao et al, 2017) (Neto et al, 2016). Among them, microfluidics is a promising technology that enables a precise control and manipulation of fluids at microliter/nanoliter range, being the most promising approach to produce monodisperse functionalized microgel capsules and particles(Q.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional laminarin microparticles for cell adhesion and expansion

Martins

Custódio

Mano

2018

Carbohydrate Polymers

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Microfabrication technologies have been widely explored to produce microgels that can be assembled in functional constructs for tissue engineering and regenerative medicine applications. Here, we propose microfluidics coupled to a source of UV light to produce multifunctional methacrylated laminarin microparticles with narrow distribution of sizes using photopolymerization. The multifunctional microparticles were loaded with platelet lysates and further conjugated with an adhesive peptide. The adhesive peptides dictated cell adhesiveness to the laminarin microparticles, the incorporation of platelet lysates have resulted in improved cell expansion compared to clear microparticles. Overall, our findings demonstrate that multifunctional methacrylated laminarin microparticles provide an effective support for cell attachment and cell expansion. Moreover, expanded cells provide the link for microparticles aggregation resulting in robust 3D structures. This suggest the potential for using the methacrylated laminarin microplatforms capable to be assembled by the action of cells to rapidly produce large tissue engineered constructs.

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“…[8][9][10] Importantly, superwettable microchips that combine two extreme states of superhydrophobicity and superhydrophilicity in precisely two-dimensional micropatterns, [11][12][13][14] exhibit excellent ability of patterning microdroplets toward new possibilities and functionalities in a wide variety of biomedical applications including high-throughput cell patterns, [15][16][17][18] drug/cell screening, [19][20][21] and open-channel biochips for separation and diagnostic devices. [22][23][24][25] Taking advantage of enrichment and anchoring microdroplet ability of such superwettable microchips, recent efforts have shifted to modify the superhydrophilic microwells with biological entities, toward more versatile and robust sensing applications. As a result, several types of superwettable microchips have been explored depending on different detection signals such as surface-enhanced Raman scattering (SERS), [26][27][28] colorimetric, [29][30][31][32][33] and fluorescence enhancement effect, [34][35][36] to perform demanding sensing tasks.…”

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confidence: 99%

Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers

Song

Gao

et al. 2018

ACS Sens.

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Bioinspired superwettable micropatterns that combine two extreme states of superhydrophobicity and superhydrophilicity with the ability to enrich and absorb microdroplets are suitable for versatile and robust sensing applications. Here we introduce a superwettable microchip that integrates superhydrophobic–superhydrophilic micropatterns and a nanodendritic electrochemical biosensor toward the detection of prostate cancer biomarkers. On the superwettable microchip, the superhydrophobic area could confine the microdroplets in superhydrophilic microwells; such behavior is extremely helpful for reducing the amount of analytical solution. In contrast, superhydrophilic microwells exhibit a high adhesive force toward microdroplets, and the nanodendritic structures can improve probe-binding capacity and response signals, thus greatly enhancing the sensitivity. Sensitive and selective detection of prostate cancer biomarkers including miRNA-375, miRNA-141, and prostate-specific antigen on a single microchip is also achieved. Such a superwettable microchip with high sensitivity, low sample volume, and upside-down detection capability in a single microdroplet shows great potential to fabricate portable devices toward complex biosensing applications.

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Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

Cited by 85 publications

References 39 publications

Patterned Arrays of Supramolecular Microcapsules

Patterned Arrays of Supramolecular Microcapsules

Multifunctional laminarin microparticles for cell adhesion and expansion

Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers

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