Multifunctional Bioinspired Bredigite-Modified Adhesive for Bone Fracture Healing

Abstract

Despite recent advances in bone adhesives applied for full median sternotomy, the regeneration of bone defects has remained challenging since the healing process is hampered by poor adhesiveness, limited bioactivity, and lack of antibacterial functions. Bioinspired adhesives by marine organisms provide a novel concept to circumvent these problems. Herein, a dual crosslink strategy is employed in designing a multifaceted bioinspired adhesive consisting of a catechol amine-functionalized hyperbranched polymer (polydopamine-co-acrylate, PDA), bredigite (BR) nanoparticles, and Fe 3+ ions. The hybrid adhesives exhibit strong adhesion to various substrates such as poly(methyl methacrylate), glass, bone, and skin tissues through synergy between irreversible covalent and reversible noncovalent cross-linking, depending on the BR content. Noticeably, the adhesion strength of hybrid adhesives containing 2 wt % BR nanoparticles to bone tissues is 2.3 ± 0.8 MPa, which is about 3 times higher than that of pure PDA adhesives. We also demonstrate that these hybrid adhesives not only are bioactive and accelerate in vitro bone-like apatite formation but also exhibit antibacterial properties against Staphylococcus aureus, depending on the BR concentration. Furthermore, the superior cellular responses in contact with hybrid adhesives, including improved human osteosarcoma MG63 cell spreading and osteogenic differentiation, are achieved owing to the appropriate ion release and flexibility of the cross-linked double-network adhesive. In summary, multifunctional hybrid PDA/BR adhesives with appreciable osteoconductive, mechanical, and antibacterial properties represent the potential applications for median sternotomy surgery as a bone tissue adhesive.

Double-Cross-Linked Hydrogel with Long-Lasting Underwater Adhesion: Enhancement of Maxillofacial In Situ and Onlay Bone Retention

“…First, as our design principle was completely based on bone adhesive, the A-O-1.0 hydrogel has simple composition and structure, making it a feasible platform for integrating more clinical needs. Although this study mainly focuses on its adhesion function, we believe that if some bioactive compounds, like drugs, peptides, metal–organic frameworks (MOFs), or micro ribonucleic acid (RNA), are added into the hydrogel, a multifunctional bone adhesive with enhanced osteogenic and angiogenic abilities will be developed. − Moreover, the A-O-1.0 hydrogel was validated in an in situ and onlay bone adhesion model that mocked the application in maxillofacial surgery. And we look forward to exploring more potential clinical application scenarios of the hydrogel in our future research.…”

Modulating Lineage Specification in Stem Cell Differentiation via Bioelectrical Stimulation Intensity Matching

“…The increased spreading area and specific morphology is related to their potential osteogenic differentiation. [ 18 ]…”

Progress of tissue adhesives based on proteins and synthetic polymers

“…The catechols like 3,4-dihydroxy phenylalanine (DOPA) play a vital role in the interfacial attachment and enabling them to stick to many different surfaces even in various hydrophilic conditions. Interestingly, Moazami et al, prepared multifunctional mussel-inspired TAs consisting of poly (DOPA-co-acrylate)(PDA), bredigite (BR) NPs, and Fe 3+ for bone fracture healing during median stenotomy surgery [ 60 ] because the PDA has a strong adhesive property in a wet condition and the BR can accelerate the mineralization of calcined tissues with mechanical properties [ 61 ]. The TAs exhibited strong adhesion of around 45.9 Mpa to cow skin tissues through irreversible covalent and reversible noncovalent crosslinking depending on the content of BR with an acceleration of in vitro bone-like apatite formation and antibacterial properties although they did not check in vivo.…”