Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens

Singh, Rajesh Kumar; Kumar, Dilip; Singh, Pratiksha; Solanki, Manoj Kumar; Srivastava, Supriya; Kashyap, Prem Lal; Kumar, Sudheer; Srivastava, Alok Kumar; Singhal, Pradeep K.; Arora, Dilip K.

doi:10.1007/s10725-013-9870-z

Cited by 60 publications

(48 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomic DNA (gDNA) extraction was carried out as described by Pospiech and Neumann [37]. The amplified 16S rRNA gene was obtained from isolated gDNA by polymerase chain reaction (PCR) using universal primers PA and PH as described previously [46]. The presence and yield of specific PCR products was checked by 1% agarose (wt/vol) gel electrophoresis, visualized by ethidium bromide staining and ultraviolet (UV) transillumination.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Potential biocontrol and superlative plant growth promoting activity of indigenous Bacillus mojavensis PB-35(R11) of soybean (Glycine max) rhizosphere

Prajakta¹,

Suvarna²,

Raghvendra

et al. 2019

SN Appl. Sci.

View full text Add to dashboard Cite

Disease control using microbes that exhibit beneficial effects on plants to strengthen the host is a foremost requirement in agriculture. The aim of the present study is to search for an effective biocontrol agent against Rhizoctonia solani endowed with high plant growth potential. A total of 95 bacterial strains were isolated from the soybean plant rhizosphere and screened in vitro against R. solani by dual culture technique, revealing that strain PB-35(R11) was the most efficient for controlling R. solani with 54.835% inhibition. Isolate PB-35 was identified by 16S ribosomal RNA (rRNA) gene sequencing as Bacillus mojavensis. It was observed that, in order to effectively control R. solani in vitro, B. mojavensis produced volatile metabolites. Fourier-transform infrared (FTIR) analysis revealed the presence of aldehyde (CHO) group (1739.79 cm −1), acetyl group (1896.03 cm −1), and cyanide group (2360.87 cm −1) in the crude extract of isolate PB-35(R11). Furthermore, the siderophore, indole acetic acid (IAA), catalase, oxidase, and chitinase production ability as well as phosphate solubilization potential of PB-35(R11) make it beneficial for crop growth and soil biofortification.

show abstract

Section: Molecular Characterizationmentioning

confidence: 99%

Potential biocontrol and superlative plant growth promoting activity of indigenous Bacillus mojavensis PB-35(R11) of soybean (Glycine max) rhizosphere

Prajakta¹,

Suvarna²,

Raghvendra

et al. 2019

SN Appl. Sci.

View full text Add to dashboard Cite

show abstract

“…This has already resulted in incorrect identification. In recent years, the usefulness of molecular markers such as random amplified polymorphic DNA (RAPD) and repetitive-element polymerase chain reaction (REP-PCR) in resolving species differences among microbial species are also well documented (Sharma et al 2009; Solanki et al 2013; Srivastava et al 2014; Singh et al 2014; Kashyap et al 2015). RAPD utilized PCR to amplify DNA segments with single primer of arbitrary nucleotide sequence generating fragments by hybridizing with compatible regions of DNA and amplifying the regions where the primers are in correct orientation and appropriately spaced (100–2500 bp).…”

Section: Introductionmentioning

confidence: 99%

Identification, characterization and phylogenetic analysis of antifungal Trichoderma from tomato rhizosphere

et al. 2016

Self Cite

View full text Add to dashboard Cite

The use of Trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. In this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (Fusarium oxysporum f. sp. lycopersici, Alternaria alternata, Colletotrichum gloeosporoides and Rhizoctonia solani). The production of extracellular cell wall degrading enzymes of tested isolates was also measured. All the isolates significantly reduced the mycelial growth of tested pathogens but the amount of growth reduction varied significantly as well. There was a positive correlation between the antagonistic capacity of Trichoderma isolates towards fungal pathogens and their lytic enzyme production. The Trichoderma isolates were initially sorted according to morphology and based on the translation elongation factor 1-α gene sequence similarity, the isolates were designated as Trichoderma harzianum, T. koningii, T. asperellum, T. virens and T. viride. PCA analysis explained 31.53, 61.95, 62.22 and 60.25% genetic variation among Trichoderma isolates based on RAPD, REP-, ERIC- and BOX element analysis, respectively. ERG-1 gene, encoding a squalene epoxidase has been used for the first time for diversity analysis of antagonistic Trichoderma from tomato rhizosphere. Phylogenetic analysis of ERG-1 gene sequences revealed close relatedness of ERG-1sequences with earlier reported sequences of Hypocrea lixii, T. arundinaceum and T. reesei. However, ERG-1 gene also showed heterogeneity among some antagonistic isolates and indicated the possibility of occurrence of squalene epoxidase driven triterpene biosynthesis as an alternative biocontrol mechanism in Trichoderma species.

show abstract

“…1). Results pertaining to the growth of isolate AP at different temperatures (24,30,37, and 40°C) and NaCl concentrations (0, 2, 4, 6, 8, and 10%; w/v) are shown in Figs. 2 and 3, respectively.…”

Section: Resultsmentioning

confidence: 99%

Deciphering the salinity adaptation mechanism in Penicilliopsis clavariiformis AP, a rare salt tolerant fungus from mangrove

Kashyap

Rai

Singh

et al. 2015

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

Penicilliopsis clavariiformis AP, a rare salt tolerant fungus reported for the first time from India was identified through polyphasic taxonomy. Scanning electron microscopy showed that the fungus has unique features such as biverticillate penicilli bearing masses of oval to ellipsoidal conidia. The fungus has been characterized for salt tolerance and to understand the relevance of central carbon metabolism in salt stress adaptation. It showed optimal growth at 24 °C and able to tolerate up to 10% (w/v) NaCl. To understand the mechanism of adaptation to high salinity, activities of the key enzymes regulating glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle were investigated under normal (0% NaCl) and saline stress environment (10% NaCl). The results revealed a re-routing of carbon metabolism away from glycolysis to the pentose phosphate pathway (PPP), served as a cellular stress-resistance mechanism in fungi under saline environment. The detection and significant expression of fungus genes (Hsp98, Hsp60, HTB, and RHO) under saline stress suggest that these halotolerance conferring genes from the fungus could have a role in fungus protection and adaptation under saline environment. Overall, the present findings indicate that the rearrangement of the metabolic fluxes distribution and stress related genes play an important role in cell survival and adaptation under saline environment.

show abstract

Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens

Cited by 60 publications

References 28 publications

Potential biocontrol and superlative plant growth promoting activity of indigenous Bacillus mojavensis PB-35(R11) of soybean (Glycine max) rhizosphere

Potential biocontrol and superlative plant growth promoting activity of indigenous Bacillus mojavensis PB-35(R11) of soybean (Glycine max) rhizosphere

Identification, characterization and phylogenetic analysis of antifungal Trichoderma from tomato rhizosphere

Deciphering the salinity adaptation mechanism in Penicilliopsis clavariiformis AP, a rare salt tolerant fungus from mangrove

Contact Info

Product

Resources

About