Multifarious cholesterol lowering potential of lactic acid bacteria equipped with desired probiotic functional attributes

Bhat, Bilal A.; Bajaj, Bijender Kumar

doi:10.1007/s13205-020-02183-8

Cited by 30 publications

(25 citation statements)

References 46 publications

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“…Several mechanisms for cholesterol reduction by lactic acid bacteria (LAB) have been proposed, such as deconjugation of bile salts by bile-salt hydrolase (BSH) (22)(23)(24), binding and incorporation of cholesterol to the LAB cellular surface (25)(26)(27), production of short-chain fatty acids (SCFAs) during the LAB growth (28,29), and co-precipitation of cholesterol with deconjugated bile salts (30,31). Nevertheless, the mechanism for cholesterol reduction by LAB needs to be studied on a case-tocase basis and the cholesterol-lowering effects of LAB still need to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Probiotic Effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on Hypercholesteremic Golden Hamsters

Yang

Lyu

et al. 2021

Front. Nutr.

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Hypercholesteremia or high cholesterol is one of the important factors leading to atherosclerosis and other cardiovascular diseases. The application of probiotics with cholesterol-lowering characteristics has become increasingly popular over the past decade due to their contribution to human health. This study aimed to evaluate the probiotic effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on hyperlipidemic golden hamsters. A hyperlipidemic model was established through a high cholesterol diet in golden hamsters, after which lyophilized Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 were orally administered individually for 8 weeks. The physiological characteristics of golden hamsters and short chain fatty acid (SCFA) in the colon were assessed by automatic Biochemical Analyzer and gas choromatograph, respectively. A MiSeq sequencing-based analysis of the bacterial 16S rRNA gene (V3–V4 region) in the cecum content was performed to analyze the cecum microbiota. Correlations between sets of these variables were also investigated using the R package “corrplot.” Results showed that neither Lactobacillus fermentum ZJUIDS06 nor Lactobacillus plantarum ZY08 inhibited body weight increase. However, supplementation with Lactobacillus fermentum ZJUIDS06 for 8 weeks increased colon SCFA levels (P < 0.05), decreased serum low-density lipoprotein, total cholesterol, and triglycerides levels, and also induced changes in the cecum microbiota of hyperlipidemic golden hamsters. Remarkably, oral administration of Lactobacillus fermentum ZJUIDS06 increased the relative abundance of Parabacteroides in the cecum, which served as a biomarker for colon SCFA production and improvement of serum cholesterol levels. In a word, Lactobacillus fermentum ZJUIDS06 improved hyperlipidemia in golden hamsters, which correlated with an increase in SCFA levels and relative abundance of Parabacteroides, indicating its potential importance in functional foods that can help lower cholesterol.

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Section: Introductionmentioning

confidence: 99%

Probiotic Effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on Hypercholesteremic Golden Hamsters

Yang

Lyu

et al. 2021

Front. Nutr.

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“…In their study, Shimizu et al (2015) reported that consumption of probiotics by elderly and hypercholesterolaemic patients could be more effective than in youngsters and individuals with normal lipid levels. Besides, probiotics can reduce cholesterol levels by assimilating and entrapping this molecule into bacterial membranes (Castorena-Alba et al 2018;Bhat and Bajaj 2020). There are numerous species of Lactobacillus and Bifidobacterium that have shown cholesterol assimilation in in-vitro experiments and it has been reported that this ability is strictly strain-…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus paracasei DTA81, a cholesterol‐lowering strain having immunomodulatory activity, reveals gut microbiota regulation capability in BALB/c mice receiving high‐fat diet

et al. 2021

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Aims: In-vitro/In-vivo evaluation of cholesterol-lowering probiotic strain Lactobacillus paracasei DTA81 and the possible connection with the gut microbiota modulation. Methods and Results: In the present study, strain DTA81 has been evaluated for the possible influence on blood lipid and glucose concentrations, modulation of the immune system, gastrointestinal survivability and modulation of gut microbiota in BALB/c mice receiving a high-fat diet. After 6 weeks of treatment, a significant reduction of total cholesterol and fasting blood sugar (FBS) among animals treated with L. paracasei DTA81 has been recorded. Comparison of colon tissue levels of different cytokines revealed a significant reduction of the inflammatory cytokine interleukin-6. The comparison of gut microbiota using the 16S rRNA approach indicated that the treatment with L. paracasei DTA81 significantly increased the taxa Bacteroidetes and Coprococcus. Moreover, the genome of DTA81 was sequenced for the insilico assessment, and the analysis indicated the presence of cholesterol assimilation-related genes as well as the absence of negative traits such as transmissible antibiotic resistance genes, plasmids and prophage regions. Conclusion:The outcome of this study revealed the in-vitro and in-vivo properties of L. paracasei DTA81 and the possible mechanism between consumption of this strain, the abundance of Bacteriodetes/Coprococcus taxa, immunomodulatory activity and the subsequent reduction of cholesterol/FBS in BALB/c mice. Significance and Impact of the Study: Lactobacillus paracasei DTA81 as a nonpharmacological potential probiotic supplement can influence metabolic homeostasis in individuals, particularly those adopting high-fat diets, and it can contribute to reduce coronary heart disease.

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“…Human milk is the gold standard for infant nutrition [30], and a number of commensal lactobacillus and bifidobacterial strains have been isolated and found to have probiotic properties [31]. Isolating new probiotics from human milk is desirable because of safety and adaptation to the human GIT [6], which remains an underexplored realm in the South Asian context [32]. Choosing tertiary hospitals in major urban centres allowed a representative sampling of mothers who frequent them for obstetrical purposes [33].…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus commensals autochthonous to human milk have the hallmarks of potent probiotics

et al. 2020

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Maternal milk is an important source of essential nutrients for the optimal growth of infants. Breastfeeding provides a continuous supply of beneficial bacteria to colonize the infant gastrointestinal tract (GIT) and offers health benefits for disease prevention and immunity. The purpose of this study was to isolate novel probiotic strains from the breast milk of native Pakistani mothers and to evaluate their probiotic potential. We isolated 21 strains of bacteria from the colostrum and mature milk of 20 healthy mothers, who had vaginal deliveries and were not taking antibiotics. After phenotypic and genotypic characterization, these isolates were tested for survival in the GIT using in vitro acid and bile tests. Nine strains showing good acid tolerance were assessed for their growth rate, bile resistance and ability to hydrolyze bile salts. Out of the four Lactobacillus isolates adjudged to be most promising as probiotics, three were Lactobacillus fermentum strains and one was a strain of Lactobacillus oris . This study demonstrates that human milk is a viable source of commensal bacteria beneficial to both adults and babies.

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Multifarious cholesterol lowering potential of lactic acid bacteria equipped with desired probiotic functional attributes

Cited by 30 publications

References 46 publications

Probiotic Effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on Hypercholesteremic Golden Hamsters

Probiotic Effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on Hypercholesteremic Golden Hamsters

Lactobacillus paracasei DTA81, a cholesterol‐lowering strain having immunomodulatory activity, reveals gut microbiota regulation capability in BALB/c mice receiving high‐fat diet

Lactobacillus commensals autochthonous to human milk have the hallmarks of potent probiotics

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